Functional Asymmetries Routing the Mating Behavior of the Rusty Grain Beetle, Cryptolestes ferrugineus (Stephens) (Coleoptera: Laemophloeidae)

SIMPLE SUMMARY: We evaluated the behavioral asymmetries of Cryptolestes ferrugineus (Stephens) (Coleoptera: Laemophloeidae) males during courtship and mating with potential mates. The highest proportion of males showed left-biased approaches towards females, and turned 180° to their left. Right-biased males (i.e., approaching mates from the right and then turning 180°) were fewer than left-biased males. A low percentage of males approaching from the front and back side achieved successful mating. Left-biased-approaching males had a significantly shorter copula duration in comparison with other males. Left-biased males performed shorter copulation attempts and copula in comparison to right-biased males. This research contributes to understand the role of lateralization in the beetle family Laemophloeidae. ABSTRACT: The rusty grain beetle, Cryptolestes ferrugineus (Stephens) (Coleoptera: Laemophloeidae), is a serious secondary pest of stored and processed food commodities. In the present study, we investigated the lateralization of males during courtship and mating, attempting to understand if it can be linked with a high likelihood of successful copulation. Most males exhibited left-biased (41%) approaches towards females, and turned 180° to their left, with 37% mating success. Right-biased males (i.e., approaching from the right and then turning 180°) were fewer than left-biased ones; 26% out of 34% managed to copulate with females. Only 9% out of 13% and 7% out of 11% of the back side- and front side-approaching males succeeded in mating, respectively. Directional asymmetries in approaching a potential mate, as well as the laterality of side-biased turning 180°, significantly affected male copulation success, with left-biased males achieving higher mating success if compared to right-biased males. Copula duration was significantly lower for left-biased-approaching males (1668.0 s) over the others (i.e., 1808.1, 1767.9 and 1746.9 for right-biased, front and back side-males, respectively). Left-biased males performed shorter copulation attempts and copula compared to right-biased males. Overall, our study adds basic knowledge to the lateralized behavioral displays during courtship and copula of C. ferrugineus.