Control Efficacy of Entomopathogenic Fungus Purpureocillium lilacinum against Chili Thrips (Scirtothrips dorsalis) on Chili Plant

SIMPLE SUMMARY: Chili thrips (Scirtothrips dorsalis) is an important pest of chili crops and a major vector of viral plant pathogens. Due to the widespread outbreak of thrips, chemical insecticides have been heavily used in the last few decades. To reduce the utilization of chemical pesticides, alternative biocontrol agents such as entomopathogenic fungi have been screened against the thrips. Laboratory screening revealed that 2 insect fungi isolates, Purpureocillium lilacinum TBRC 10638 and Beauveria bassiana BCC48145 were the most effective isolates against chili thrips. The fungus, P. lilacinum TBRC 10638 exhibited the highest efficacy against chili thrips in greenhouse and field trials and thus would be developed as a thrips control agent. ABSTRACT: In a laboratory assay, it was shown that B. bassiana BCC48145, BCC2660, and P. lilacinum TBRC10638 were the three strains that exhibited the highest insecticidal activity against chili thrips, causing 92.5% and 91.86% and 92.3% corrected mortality, respectively. The fungi B. bassiana BCC48145 and P. lilacinum TBRC10638 were selected for greenhouse spraying. Cytotoxicity test of the extracts from both fungi evaluated against 4 animal cell lines: KB; human oral cavity carcinoma, MCF7; human breast adenocarcinoma, NCI-H187; human small cell lung carcinoma and GFP-expressing Vero cells, showed none-cytotoxic to all cell lines. An efficacy validation in the greenhouse showed that P. lilacinum TBRC 10638 was more effective than B. bassiana BCC48145 and could control the thrips up to 80% when using the fungus at 10(8) spores/mL. The LC(50) values of P. lilacinum TBRC 10638 against chili thrips based on total thrips count from two experiments were 1.42 × 10(8) and 1.12 × 10(7) spores/mL when the fungal spores were sprayed once a week. The optimal concentration of P. lilacinum TBRC 10638 spores for effective control of chili thrips was determined at 1.41 × 10(9) spores/mL. The average efficacy of P. lilacinum TBRC 10638 for thrips control from 3 field trials was 30.08%, 14.39%, and 29.92%. This result was not significantly different from that of the chemical insecticide treatment group, which showed efficacy at 19.27%, 14.92%, and 19.97%. Furthermore, there was no difference in productivity among the different treatment groups. Our results demonstrated that P. lilacinum TBRC 10638 is a promising biocontrol agent that could be used as an alternative to chemical insecticide for controlling chili thrips.