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Targeted Modification of Mammalian DNA by a Novel Type V Cas12a Endonuclease from Ruminococcus bromii

Type V Cas12a nucleases are DNA editors working in a wide temperature range and using expanded protospacer-adjacent motifs (PAMs). Though they are widely used, there is still a demand for discovering new ones. Here, we demonstrate a novel ortholog from Ruminococcus bromii sp. entitled RbCas12a, whic...

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Detalles Bibliográficos
Autores principales:	Vasilev, Ruslan, Gunitseva, Natalia, Shebanova, Regina, Korzhenkov, Aleksei, Vlaskina, Anna, Evteeva, Marta, Polushkina, Irina, Nikitchina, Natalia, Toshchakov, Stepan, Kamenski, Piotr, Patrushev, Maxim, Mazunin, Ilya
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409102/ https://www.ncbi.nlm.nih.gov/pubmed/36012553 http://dx.doi.org/10.3390/ijms23169289

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