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Anti-Inflammatory Effect of Topiramate in a Chronic Model of TNBS-Induced Colitis

Inflammatory bowel disease (IBD) is characterized by a chronic and relapsing inflammatory response in the gastrointestinal tract, resulting in severe symptoms such as abdominal pain, vomiting, diarrhea, bloody stools, and weight loss. Currently, there is no cure, and the pharmacological treatment in...

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Autores principales: Silva, Inês, Mendes, Priscila, Guerra, Sofia, Pinto, Rui, Mateus, Vanessa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409153/
https://www.ncbi.nlm.nih.gov/pubmed/36012393
http://dx.doi.org/10.3390/ijms23169127
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author Silva, Inês
Mendes, Priscila
Guerra, Sofia
Pinto, Rui
Mateus, Vanessa
author_facet Silva, Inês
Mendes, Priscila
Guerra, Sofia
Pinto, Rui
Mateus, Vanessa
author_sort Silva, Inês
collection PubMed
description Inflammatory bowel disease (IBD) is characterized by a chronic and relapsing inflammatory response in the gastrointestinal tract, resulting in severe symptoms such as abdominal pain, vomiting, diarrhea, bloody stools, and weight loss. Currently, there is no cure, and the pharmacological treatment includes drugs that induce and keep the patient in remission, not reversing the underlying pathogenic mechanism. These therapies, in the long term, may cause various side effects and complications, which has increased the need to investigate new, more effective, and safer pharmacological approaches. In preclinical studies, topiramate has demonstrated a potential anti-inflammatory effect by inhibiting the production of several pro-inflammatory cytokines. This study aimed to investigate the effect of topiramate in a chronic TNBS-induced colitis model in rodents. Experimental colitis was induced by four intrarectal administrations of 1% TNBS in female CD-1 mice. Topiramate 10 and 20 mg were administered intraperitoneally for 14 days. Several parameters were evaluated, such as bodyweight, alkaline phosphatase (ALP), fecal hemoglobin, fecal calprotectin, tumor necrosis factor (TNF)-α, and interleukin (IL)-10. Topiramate reduces TNBS-induced colonic damage in a model of chronic experimental colitis and normalizes the stool consistency and anus appearance. Additionally, topiramate significantly reduced the concentration of ALP, fecal hemoglobin, fecal calprotectin, TNF-α, and IL-10, demonstrating it to be a promising pharmacological approach for the treatment of IBD in the future.
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spelling pubmed-94091532022-08-26 Anti-Inflammatory Effect of Topiramate in a Chronic Model of TNBS-Induced Colitis Silva, Inês Mendes, Priscila Guerra, Sofia Pinto, Rui Mateus, Vanessa Int J Mol Sci Article Inflammatory bowel disease (IBD) is characterized by a chronic and relapsing inflammatory response in the gastrointestinal tract, resulting in severe symptoms such as abdominal pain, vomiting, diarrhea, bloody stools, and weight loss. Currently, there is no cure, and the pharmacological treatment includes drugs that induce and keep the patient in remission, not reversing the underlying pathogenic mechanism. These therapies, in the long term, may cause various side effects and complications, which has increased the need to investigate new, more effective, and safer pharmacological approaches. In preclinical studies, topiramate has demonstrated a potential anti-inflammatory effect by inhibiting the production of several pro-inflammatory cytokines. This study aimed to investigate the effect of topiramate in a chronic TNBS-induced colitis model in rodents. Experimental colitis was induced by four intrarectal administrations of 1% TNBS in female CD-1 mice. Topiramate 10 and 20 mg were administered intraperitoneally for 14 days. Several parameters were evaluated, such as bodyweight, alkaline phosphatase (ALP), fecal hemoglobin, fecal calprotectin, tumor necrosis factor (TNF)-α, and interleukin (IL)-10. Topiramate reduces TNBS-induced colonic damage in a model of chronic experimental colitis and normalizes the stool consistency and anus appearance. Additionally, topiramate significantly reduced the concentration of ALP, fecal hemoglobin, fecal calprotectin, TNF-α, and IL-10, demonstrating it to be a promising pharmacological approach for the treatment of IBD in the future. MDPI 2022-08-15 /pmc/articles/PMC9409153/ /pubmed/36012393 http://dx.doi.org/10.3390/ijms23169127 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Silva, Inês
Mendes, Priscila
Guerra, Sofia
Pinto, Rui
Mateus, Vanessa
Anti-Inflammatory Effect of Topiramate in a Chronic Model of TNBS-Induced Colitis
title Anti-Inflammatory Effect of Topiramate in a Chronic Model of TNBS-Induced Colitis
title_full Anti-Inflammatory Effect of Topiramate in a Chronic Model of TNBS-Induced Colitis
title_fullStr Anti-Inflammatory Effect of Topiramate in a Chronic Model of TNBS-Induced Colitis
title_full_unstemmed Anti-Inflammatory Effect of Topiramate in a Chronic Model of TNBS-Induced Colitis
title_short Anti-Inflammatory Effect of Topiramate in a Chronic Model of TNBS-Induced Colitis
title_sort anti-inflammatory effect of topiramate in a chronic model of tnbs-induced colitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409153/
https://www.ncbi.nlm.nih.gov/pubmed/36012393
http://dx.doi.org/10.3390/ijms23169127
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