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Dynamics of the Glycogen β-Particle Number in Rat Hepatocytes during Glucose Refeeding

Glycogen is an easily accessible source of energy for various processes. In hepatocytes, it can be found in the form of individual molecules (β-particles) and their agglomerates (α-particles). The glycogen content in hepatocytes depends on the physiological state and can vary due to the size and num...

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Autores principales: Bezborodkina, Natalia N., Stepanov, Andrei V., Vorobev, Mikhail L., Stein, Grigory I., Okovityi, Sergey V., Kudryavtsev, Boris N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409161/
https://www.ncbi.nlm.nih.gov/pubmed/36012534
http://dx.doi.org/10.3390/ijms23169263
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author Bezborodkina, Natalia N.
Stepanov, Andrei V.
Vorobev, Mikhail L.
Stein, Grigory I.
Okovityi, Sergey V.
Kudryavtsev, Boris N.
author_facet Bezborodkina, Natalia N.
Stepanov, Andrei V.
Vorobev, Mikhail L.
Stein, Grigory I.
Okovityi, Sergey V.
Kudryavtsev, Boris N.
author_sort Bezborodkina, Natalia N.
collection PubMed
description Glycogen is an easily accessible source of energy for various processes. In hepatocytes, it can be found in the form of individual molecules (β-particles) and their agglomerates (α-particles). The glycogen content in hepatocytes depends on the physiological state and can vary due to the size and number of the particles. Using biochemical, cytofluorometric, interferometric and morphometric methods, the number of β-particles in rat hepatocytes was determined after 48 h of fasting at different time intervals after glucose refeeding. It has been shown that after starvation, hepatocytes contain ~1.6 × 10(8) β-particles. During refeeding, their number of hepatocytes gradually increases and reaches a maximum (~5.9 × 10(8)) at 45 min after glucose administration, but then quickly decreases. The data obtained suggest that in cells there is a continuous synthesis and degradation of particles, and at different stages of life, one or another process predominates. It has been suggested that in the course of glycogenesis, pre-existing β-particles are replaced by those formed de novo. The main contribution to the deposition of glycogen is made by an increase in the glucose residue number in its molecules. The average diameter of β-particles of glycogen during glycogenesis increases from ~11 nm to 21 nm.
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spelling pubmed-94091612022-08-26 Dynamics of the Glycogen β-Particle Number in Rat Hepatocytes during Glucose Refeeding Bezborodkina, Natalia N. Stepanov, Andrei V. Vorobev, Mikhail L. Stein, Grigory I. Okovityi, Sergey V. Kudryavtsev, Boris N. Int J Mol Sci Article Glycogen is an easily accessible source of energy for various processes. In hepatocytes, it can be found in the form of individual molecules (β-particles) and their agglomerates (α-particles). The glycogen content in hepatocytes depends on the physiological state and can vary due to the size and number of the particles. Using biochemical, cytofluorometric, interferometric and morphometric methods, the number of β-particles in rat hepatocytes was determined after 48 h of fasting at different time intervals after glucose refeeding. It has been shown that after starvation, hepatocytes contain ~1.6 × 10(8) β-particles. During refeeding, their number of hepatocytes gradually increases and reaches a maximum (~5.9 × 10(8)) at 45 min after glucose administration, but then quickly decreases. The data obtained suggest that in cells there is a continuous synthesis and degradation of particles, and at different stages of life, one or another process predominates. It has been suggested that in the course of glycogenesis, pre-existing β-particles are replaced by those formed de novo. The main contribution to the deposition of glycogen is made by an increase in the glucose residue number in its molecules. The average diameter of β-particles of glycogen during glycogenesis increases from ~11 nm to 21 nm. MDPI 2022-08-17 /pmc/articles/PMC9409161/ /pubmed/36012534 http://dx.doi.org/10.3390/ijms23169263 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bezborodkina, Natalia N.
Stepanov, Andrei V.
Vorobev, Mikhail L.
Stein, Grigory I.
Okovityi, Sergey V.
Kudryavtsev, Boris N.
Dynamics of the Glycogen β-Particle Number in Rat Hepatocytes during Glucose Refeeding
title Dynamics of the Glycogen β-Particle Number in Rat Hepatocytes during Glucose Refeeding
title_full Dynamics of the Glycogen β-Particle Number in Rat Hepatocytes during Glucose Refeeding
title_fullStr Dynamics of the Glycogen β-Particle Number in Rat Hepatocytes during Glucose Refeeding
title_full_unstemmed Dynamics of the Glycogen β-Particle Number in Rat Hepatocytes during Glucose Refeeding
title_short Dynamics of the Glycogen β-Particle Number in Rat Hepatocytes during Glucose Refeeding
title_sort dynamics of the glycogen β-particle number in rat hepatocytes during glucose refeeding
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409161/
https://www.ncbi.nlm.nih.gov/pubmed/36012534
http://dx.doi.org/10.3390/ijms23169263
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