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MoDHX35, a DEAH-Box Protein, Is Required for Appressoria Formation and Full Virulence of the Rice Blast Fungus, Magnaporthe oryzae

The DExD/H-box protein family encompasses a large number of RNA helicases that are involved in RNA metabolism and a variety of physiological functions in different species. However, there is limited knowledge of whether DExD/H-box proteins play a role in the pathogenicity of plant fungal pathogens....

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Autores principales: Ying, Shumin, Zhang, Zhen, Zhang, Yanan, Hao, Zhongna, Chai, Rongyao, Qiu, Haiping, Wang, Yanli, Zhu, Xueming, Wang, Jiaoyu, Sun, Guochang, Lin, Fucheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409162/
https://www.ncbi.nlm.nih.gov/pubmed/36012276
http://dx.doi.org/10.3390/ijms23169015
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author Ying, Shumin
Zhang, Zhen
Zhang, Yanan
Hao, Zhongna
Chai, Rongyao
Qiu, Haiping
Wang, Yanli
Zhu, Xueming
Wang, Jiaoyu
Sun, Guochang
Lin, Fucheng
author_facet Ying, Shumin
Zhang, Zhen
Zhang, Yanan
Hao, Zhongna
Chai, Rongyao
Qiu, Haiping
Wang, Yanli
Zhu, Xueming
Wang, Jiaoyu
Sun, Guochang
Lin, Fucheng
author_sort Ying, Shumin
collection PubMed
description The DExD/H-box protein family encompasses a large number of RNA helicases that are involved in RNA metabolism and a variety of physiological functions in different species. However, there is limited knowledge of whether DExD/H-box proteins play a role in the pathogenicity of plant fungal pathogens. In the present work, the DExD/H-box protein MoDHX35, which belongs to the DEAH subfamily, was shown to be crucial in appressoria formation and full virulence of the rice blast fungus, Magnaporthe oryzae. The predicted protein sequence of MoDHX35 had typical DEAH-box domains, showed 47% identity to DHX35 in Homo species, but had no orthologs in Saccharomyces cerevisiae. Deletion of the MoDHX35 gene resulted in reduced tolerance of the mutants to doxorubicin, a nucleic acid synthesis disturbing agent, suggesting the involvement of MoDHX35 in RNA metabolism. MoDHX35-deleted mutants exhibited normal vegetative growth, conidia generation and conidial germination, but showed a reduced appressorium formation rate and attenuated virulence. Our work demonstrates the involvement of DEAH-box protein functions in the pathogenicity of plant fungal pathogens.
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spelling pubmed-94091622022-08-26 MoDHX35, a DEAH-Box Protein, Is Required for Appressoria Formation and Full Virulence of the Rice Blast Fungus, Magnaporthe oryzae Ying, Shumin Zhang, Zhen Zhang, Yanan Hao, Zhongna Chai, Rongyao Qiu, Haiping Wang, Yanli Zhu, Xueming Wang, Jiaoyu Sun, Guochang Lin, Fucheng Int J Mol Sci Article The DExD/H-box protein family encompasses a large number of RNA helicases that are involved in RNA metabolism and a variety of physiological functions in different species. However, there is limited knowledge of whether DExD/H-box proteins play a role in the pathogenicity of plant fungal pathogens. In the present work, the DExD/H-box protein MoDHX35, which belongs to the DEAH subfamily, was shown to be crucial in appressoria formation and full virulence of the rice blast fungus, Magnaporthe oryzae. The predicted protein sequence of MoDHX35 had typical DEAH-box domains, showed 47% identity to DHX35 in Homo species, but had no orthologs in Saccharomyces cerevisiae. Deletion of the MoDHX35 gene resulted in reduced tolerance of the mutants to doxorubicin, a nucleic acid synthesis disturbing agent, suggesting the involvement of MoDHX35 in RNA metabolism. MoDHX35-deleted mutants exhibited normal vegetative growth, conidia generation and conidial germination, but showed a reduced appressorium formation rate and attenuated virulence. Our work demonstrates the involvement of DEAH-box protein functions in the pathogenicity of plant fungal pathogens. MDPI 2022-08-12 /pmc/articles/PMC9409162/ /pubmed/36012276 http://dx.doi.org/10.3390/ijms23169015 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ying, Shumin
Zhang, Zhen
Zhang, Yanan
Hao, Zhongna
Chai, Rongyao
Qiu, Haiping
Wang, Yanli
Zhu, Xueming
Wang, Jiaoyu
Sun, Guochang
Lin, Fucheng
MoDHX35, a DEAH-Box Protein, Is Required for Appressoria Formation and Full Virulence of the Rice Blast Fungus, Magnaporthe oryzae
title MoDHX35, a DEAH-Box Protein, Is Required for Appressoria Formation and Full Virulence of the Rice Blast Fungus, Magnaporthe oryzae
title_full MoDHX35, a DEAH-Box Protein, Is Required for Appressoria Formation and Full Virulence of the Rice Blast Fungus, Magnaporthe oryzae
title_fullStr MoDHX35, a DEAH-Box Protein, Is Required for Appressoria Formation and Full Virulence of the Rice Blast Fungus, Magnaporthe oryzae
title_full_unstemmed MoDHX35, a DEAH-Box Protein, Is Required for Appressoria Formation and Full Virulence of the Rice Blast Fungus, Magnaporthe oryzae
title_short MoDHX35, a DEAH-Box Protein, Is Required for Appressoria Formation and Full Virulence of the Rice Blast Fungus, Magnaporthe oryzae
title_sort modhx35, a deah-box protein, is required for appressoria formation and full virulence of the rice blast fungus, magnaporthe oryzae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409162/
https://www.ncbi.nlm.nih.gov/pubmed/36012276
http://dx.doi.org/10.3390/ijms23169015
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