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Metabolomic Analysis Revealed Distinct Physiological Responses of Leaves and Roots to Huanglongbing in a Citrus Rootstock

Huanglongbing (HLB) is an obstinate disease in the citrus industry. No resistant citrus resources were currently available, but various degrees of Huanglongbing tolerance exist in different germplasm. Citrus junos is emerging as one of the popular rootstocks widely used in the citrus production. How...

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Autores principales: Chen, Qing, Min, Ailing, Luo, Shu, He, Jinwei, Wu, Runqin, Lin, Ximeng, Wang, Yan, He, Wen, Zhang, Yunting, Lin, Yuanxiu, Li, Mengyao, Zhang, Yong, Luo, Ya, Tang, Haoru, Wang, Xiaorong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409271/
https://www.ncbi.nlm.nih.gov/pubmed/36012507
http://dx.doi.org/10.3390/ijms23169242
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author Chen, Qing
Min, Ailing
Luo, Shu
He, Jinwei
Wu, Runqin
Lin, Ximeng
Wang, Yan
He, Wen
Zhang, Yunting
Lin, Yuanxiu
Li, Mengyao
Zhang, Yong
Luo, Ya
Tang, Haoru
Wang, Xiaorong
author_facet Chen, Qing
Min, Ailing
Luo, Shu
He, Jinwei
Wu, Runqin
Lin, Ximeng
Wang, Yan
He, Wen
Zhang, Yunting
Lin, Yuanxiu
Li, Mengyao
Zhang, Yong
Luo, Ya
Tang, Haoru
Wang, Xiaorong
author_sort Chen, Qing
collection PubMed
description Huanglongbing (HLB) is an obstinate disease in the citrus industry. No resistant citrus resources were currently available, but various degrees of Huanglongbing tolerance exist in different germplasm. Citrus junos is emerging as one of the popular rootstocks widely used in the citrus production. However, its responses to the HLB causal agent, Candidatus Liberibacter asiaticus (CLas), were still elusive. In the current study, we investigated the physiological, anatomical, and metabolomic responses of a C. junos rootstock ‘Pujiang Xiangcheng’ by a controlled CLas grafting inoculation. The summer flushes and roots were impaired at 15 weeks after inoculation, although typical leaf symptomatic phenotypes were not obvious. The chlorophyll pigments and the photosynthetic rate were compromised. The phloem sieve tubes were still working, despite the fact that the callose was deposited and the starch granules were accumulated in the phloem cells. A wide, targeted metabolomic analysis was carried out to explore the systematic alterations of the metabolites at this early stage of infection in the leaves and root system. The differentially accumulated metabolites in the CLas-affected leaves and roots compared with the mock-inoculation control tissues revealed that distinct responses were obvious. Besides the commonly observed alteration of sugar and amino acids, the active break down of starch in the roots was discovered. The different types of fatty acids were altered in the two tissues, with a more pronounced content decline in the roots. Our results not only provided fundamental knowledge about the response of the C. junos rootstock to the HLB disease, but also presented new insights into the host–pathogen interaction in the early stages.
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spelling pubmed-94092712022-08-26 Metabolomic Analysis Revealed Distinct Physiological Responses of Leaves and Roots to Huanglongbing in a Citrus Rootstock Chen, Qing Min, Ailing Luo, Shu He, Jinwei Wu, Runqin Lin, Ximeng Wang, Yan He, Wen Zhang, Yunting Lin, Yuanxiu Li, Mengyao Zhang, Yong Luo, Ya Tang, Haoru Wang, Xiaorong Int J Mol Sci Article Huanglongbing (HLB) is an obstinate disease in the citrus industry. No resistant citrus resources were currently available, but various degrees of Huanglongbing tolerance exist in different germplasm. Citrus junos is emerging as one of the popular rootstocks widely used in the citrus production. However, its responses to the HLB causal agent, Candidatus Liberibacter asiaticus (CLas), were still elusive. In the current study, we investigated the physiological, anatomical, and metabolomic responses of a C. junos rootstock ‘Pujiang Xiangcheng’ by a controlled CLas grafting inoculation. The summer flushes and roots were impaired at 15 weeks after inoculation, although typical leaf symptomatic phenotypes were not obvious. The chlorophyll pigments and the photosynthetic rate were compromised. The phloem sieve tubes were still working, despite the fact that the callose was deposited and the starch granules were accumulated in the phloem cells. A wide, targeted metabolomic analysis was carried out to explore the systematic alterations of the metabolites at this early stage of infection in the leaves and root system. The differentially accumulated metabolites in the CLas-affected leaves and roots compared with the mock-inoculation control tissues revealed that distinct responses were obvious. Besides the commonly observed alteration of sugar and amino acids, the active break down of starch in the roots was discovered. The different types of fatty acids were altered in the two tissues, with a more pronounced content decline in the roots. Our results not only provided fundamental knowledge about the response of the C. junos rootstock to the HLB disease, but also presented new insights into the host–pathogen interaction in the early stages. MDPI 2022-08-17 /pmc/articles/PMC9409271/ /pubmed/36012507 http://dx.doi.org/10.3390/ijms23169242 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Qing
Min, Ailing
Luo, Shu
He, Jinwei
Wu, Runqin
Lin, Ximeng
Wang, Yan
He, Wen
Zhang, Yunting
Lin, Yuanxiu
Li, Mengyao
Zhang, Yong
Luo, Ya
Tang, Haoru
Wang, Xiaorong
Metabolomic Analysis Revealed Distinct Physiological Responses of Leaves and Roots to Huanglongbing in a Citrus Rootstock
title Metabolomic Analysis Revealed Distinct Physiological Responses of Leaves and Roots to Huanglongbing in a Citrus Rootstock
title_full Metabolomic Analysis Revealed Distinct Physiological Responses of Leaves and Roots to Huanglongbing in a Citrus Rootstock
title_fullStr Metabolomic Analysis Revealed Distinct Physiological Responses of Leaves and Roots to Huanglongbing in a Citrus Rootstock
title_full_unstemmed Metabolomic Analysis Revealed Distinct Physiological Responses of Leaves and Roots to Huanglongbing in a Citrus Rootstock
title_short Metabolomic Analysis Revealed Distinct Physiological Responses of Leaves and Roots to Huanglongbing in a Citrus Rootstock
title_sort metabolomic analysis revealed distinct physiological responses of leaves and roots to huanglongbing in a citrus rootstock
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409271/
https://www.ncbi.nlm.nih.gov/pubmed/36012507
http://dx.doi.org/10.3390/ijms23169242
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