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Small-Molecules as Chemiluminescent Probes to Detect Lipase Activity

The set-up of highly sensitive detection tools to evaluate lipase activity remains a central goal in different fields. In this context, we proposed new chemiluminescent 1,2-dioxetane luminophores, sharing an octanoyl triggerable group, to monitor lipase activity. We herein report the synthesis and b...

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Detalles Bibliográficos
Autores principales: La Rocca, Paolo, Mingione, Alessandra, Casati, Silvana, Ottria, Roberta, Allevi, Pietro, Ciuffreda, Pierangela, Rota, Paola
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409280/
https://www.ncbi.nlm.nih.gov/pubmed/36012304
http://dx.doi.org/10.3390/ijms23169039
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author La Rocca, Paolo
Mingione, Alessandra
Casati, Silvana
Ottria, Roberta
Allevi, Pietro
Ciuffreda, Pierangela
Rota, Paola
author_facet La Rocca, Paolo
Mingione, Alessandra
Casati, Silvana
Ottria, Roberta
Allevi, Pietro
Ciuffreda, Pierangela
Rota, Paola
author_sort La Rocca, Paolo
collection PubMed
description The set-up of highly sensitive detection tools to evaluate lipase activity remains a central goal in different fields. In this context, we proposed new chemiluminescent 1,2-dioxetane luminophores, sharing an octanoyl triggerable group, to monitor lipase activity. We herein report the synthesis and both the evaluation of their luminescence emission profile and their enzyme–substrate specificity, generated by three different commercial lipases (Candida cylindracea, Pseudomonas fluorescens, and Mucor miehei) and one esterase (porcine liver esterase, PLE, as a literature control). Remarkably, the present study confirmed the applicability of these 1,2-dioxetane luminophores as (i) highly efficient, broad-range, chemiluminescent probes for the detection and the enzymatic activity evaluation of lipases and as (ii) promising candidates for the future development of both flash- and glow-type luminescence assays.
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spelling pubmed-94092802022-08-26 Small-Molecules as Chemiluminescent Probes to Detect Lipase Activity La Rocca, Paolo Mingione, Alessandra Casati, Silvana Ottria, Roberta Allevi, Pietro Ciuffreda, Pierangela Rota, Paola Int J Mol Sci Article The set-up of highly sensitive detection tools to evaluate lipase activity remains a central goal in different fields. In this context, we proposed new chemiluminescent 1,2-dioxetane luminophores, sharing an octanoyl triggerable group, to monitor lipase activity. We herein report the synthesis and both the evaluation of their luminescence emission profile and their enzyme–substrate specificity, generated by three different commercial lipases (Candida cylindracea, Pseudomonas fluorescens, and Mucor miehei) and one esterase (porcine liver esterase, PLE, as a literature control). Remarkably, the present study confirmed the applicability of these 1,2-dioxetane luminophores as (i) highly efficient, broad-range, chemiluminescent probes for the detection and the enzymatic activity evaluation of lipases and as (ii) promising candidates for the future development of both flash- and glow-type luminescence assays. MDPI 2022-08-12 /pmc/articles/PMC9409280/ /pubmed/36012304 http://dx.doi.org/10.3390/ijms23169039 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
La Rocca, Paolo
Mingione, Alessandra
Casati, Silvana
Ottria, Roberta
Allevi, Pietro
Ciuffreda, Pierangela
Rota, Paola
Small-Molecules as Chemiluminescent Probes to Detect Lipase Activity
title Small-Molecules as Chemiluminescent Probes to Detect Lipase Activity
title_full Small-Molecules as Chemiluminescent Probes to Detect Lipase Activity
title_fullStr Small-Molecules as Chemiluminescent Probes to Detect Lipase Activity
title_full_unstemmed Small-Molecules as Chemiluminescent Probes to Detect Lipase Activity
title_short Small-Molecules as Chemiluminescent Probes to Detect Lipase Activity
title_sort small-molecules as chemiluminescent probes to detect lipase activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409280/
https://www.ncbi.nlm.nih.gov/pubmed/36012304
http://dx.doi.org/10.3390/ijms23169039
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