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Small-Molecules as Chemiluminescent Probes to Detect Lipase Activity
The set-up of highly sensitive detection tools to evaluate lipase activity remains a central goal in different fields. In this context, we proposed new chemiluminescent 1,2-dioxetane luminophores, sharing an octanoyl triggerable group, to monitor lipase activity. We herein report the synthesis and b...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409280/ https://www.ncbi.nlm.nih.gov/pubmed/36012304 http://dx.doi.org/10.3390/ijms23169039 |
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author | La Rocca, Paolo Mingione, Alessandra Casati, Silvana Ottria, Roberta Allevi, Pietro Ciuffreda, Pierangela Rota, Paola |
author_facet | La Rocca, Paolo Mingione, Alessandra Casati, Silvana Ottria, Roberta Allevi, Pietro Ciuffreda, Pierangela Rota, Paola |
author_sort | La Rocca, Paolo |
collection | PubMed |
description | The set-up of highly sensitive detection tools to evaluate lipase activity remains a central goal in different fields. In this context, we proposed new chemiluminescent 1,2-dioxetane luminophores, sharing an octanoyl triggerable group, to monitor lipase activity. We herein report the synthesis and both the evaluation of their luminescence emission profile and their enzyme–substrate specificity, generated by three different commercial lipases (Candida cylindracea, Pseudomonas fluorescens, and Mucor miehei) and one esterase (porcine liver esterase, PLE, as a literature control). Remarkably, the present study confirmed the applicability of these 1,2-dioxetane luminophores as (i) highly efficient, broad-range, chemiluminescent probes for the detection and the enzymatic activity evaluation of lipases and as (ii) promising candidates for the future development of both flash- and glow-type luminescence assays. |
format | Online Article Text |
id | pubmed-9409280 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94092802022-08-26 Small-Molecules as Chemiluminescent Probes to Detect Lipase Activity La Rocca, Paolo Mingione, Alessandra Casati, Silvana Ottria, Roberta Allevi, Pietro Ciuffreda, Pierangela Rota, Paola Int J Mol Sci Article The set-up of highly sensitive detection tools to evaluate lipase activity remains a central goal in different fields. In this context, we proposed new chemiluminescent 1,2-dioxetane luminophores, sharing an octanoyl triggerable group, to monitor lipase activity. We herein report the synthesis and both the evaluation of their luminescence emission profile and their enzyme–substrate specificity, generated by three different commercial lipases (Candida cylindracea, Pseudomonas fluorescens, and Mucor miehei) and one esterase (porcine liver esterase, PLE, as a literature control). Remarkably, the present study confirmed the applicability of these 1,2-dioxetane luminophores as (i) highly efficient, broad-range, chemiluminescent probes for the detection and the enzymatic activity evaluation of lipases and as (ii) promising candidates for the future development of both flash- and glow-type luminescence assays. MDPI 2022-08-12 /pmc/articles/PMC9409280/ /pubmed/36012304 http://dx.doi.org/10.3390/ijms23169039 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article La Rocca, Paolo Mingione, Alessandra Casati, Silvana Ottria, Roberta Allevi, Pietro Ciuffreda, Pierangela Rota, Paola Small-Molecules as Chemiluminescent Probes to Detect Lipase Activity |
title | Small-Molecules as Chemiluminescent Probes to Detect Lipase Activity |
title_full | Small-Molecules as Chemiluminescent Probes to Detect Lipase Activity |
title_fullStr | Small-Molecules as Chemiluminescent Probes to Detect Lipase Activity |
title_full_unstemmed | Small-Molecules as Chemiluminescent Probes to Detect Lipase Activity |
title_short | Small-Molecules as Chemiluminescent Probes to Detect Lipase Activity |
title_sort | small-molecules as chemiluminescent probes to detect lipase activity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409280/ https://www.ncbi.nlm.nih.gov/pubmed/36012304 http://dx.doi.org/10.3390/ijms23169039 |
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