Cargando…
Integrative Metabolome and Transcriptome Analysis Reveals the Regulatory Network of Flavonoid Biosynthesis in Response to MeJA in Camellia vietnamensis Huang
Flavonoids are secondary metabolites widely found in plants, which perform various biological activities, such as antiinflammation, antioxidation, antitumor, and so on. Camellia vietnamensis Huang, a species of oil-tea Camellia tree, is an important woody oil crop species widely planted on Hainan Is...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409299/ https://www.ncbi.nlm.nih.gov/pubmed/36012624 http://dx.doi.org/10.3390/ijms23169370 |
_version_ | 1784774817693564928 |
---|---|
author | Yan, Heqin Zheng, Wei Wang, Yong Wu, Yougen Yu, Jing Xia, Pengguo |
author_facet | Yan, Heqin Zheng, Wei Wang, Yong Wu, Yougen Yu, Jing Xia, Pengguo |
author_sort | Yan, Heqin |
collection | PubMed |
description | Flavonoids are secondary metabolites widely found in plants, which perform various biological activities, such as antiinflammation, antioxidation, antitumor, and so on. Camellia vietnamensis Huang, a species of oil-tea Camellia tree, is an important woody oil crop species widely planted on Hainan Island, which provides health benefits with its high antioxidant activity and abundant flavonoid content. However, very little is known about the overall molecular mechanism of flavonoid biosynthesis in C. vietnamensis Huang. In this study, methyl jasmonate (MeJA) is used as an inducer to change the content of secondary metabolites in C. vietnamensis. Then, the potential mechanisms of flavonoid biosynthesis in C. vietnamensis leaves in response to MeJA were analyzed by metabolomics and transcriptomics (RNA sequencing). The results showed that metabolome analysis detected 104 flavonoids and 74 fatty acyls which showed different expression patterns (increased or decreased expression). It was discovered by KEGG analysis that three differentially accumulated metabolites (cinnamaldehyde, kaempferol and quercitrin) were annotated in the phenylpropanoid biosynthesis (ko00940), flavonoid biosynthesis (ko00941), and flavone and flavonol biosynthesis (ko00944) pathways. In the transcriptome analysis, 35 different genes involved in the synthesis of flavonoids were identified by MapMan analysis. The key genes (PAL, 4CL, CCR, CHI, CHS, C4H, FLS) that might be involved in the formation of flavonoid were highly expressed after 2 h of MeJA treatment. This study provides new insights and data supporting the molecular mechanism underlying the metabolism and synthesis of flavonoids in C. vietnamensis under MeJA treatment. |
format | Online Article Text |
id | pubmed-9409299 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94092992022-08-26 Integrative Metabolome and Transcriptome Analysis Reveals the Regulatory Network of Flavonoid Biosynthesis in Response to MeJA in Camellia vietnamensis Huang Yan, Heqin Zheng, Wei Wang, Yong Wu, Yougen Yu, Jing Xia, Pengguo Int J Mol Sci Article Flavonoids are secondary metabolites widely found in plants, which perform various biological activities, such as antiinflammation, antioxidation, antitumor, and so on. Camellia vietnamensis Huang, a species of oil-tea Camellia tree, is an important woody oil crop species widely planted on Hainan Island, which provides health benefits with its high antioxidant activity and abundant flavonoid content. However, very little is known about the overall molecular mechanism of flavonoid biosynthesis in C. vietnamensis Huang. In this study, methyl jasmonate (MeJA) is used as an inducer to change the content of secondary metabolites in C. vietnamensis. Then, the potential mechanisms of flavonoid biosynthesis in C. vietnamensis leaves in response to MeJA were analyzed by metabolomics and transcriptomics (RNA sequencing). The results showed that metabolome analysis detected 104 flavonoids and 74 fatty acyls which showed different expression patterns (increased or decreased expression). It was discovered by KEGG analysis that three differentially accumulated metabolites (cinnamaldehyde, kaempferol and quercitrin) were annotated in the phenylpropanoid biosynthesis (ko00940), flavonoid biosynthesis (ko00941), and flavone and flavonol biosynthesis (ko00944) pathways. In the transcriptome analysis, 35 different genes involved in the synthesis of flavonoids were identified by MapMan analysis. The key genes (PAL, 4CL, CCR, CHI, CHS, C4H, FLS) that might be involved in the formation of flavonoid were highly expressed after 2 h of MeJA treatment. This study provides new insights and data supporting the molecular mechanism underlying the metabolism and synthesis of flavonoids in C. vietnamensis under MeJA treatment. MDPI 2022-08-19 /pmc/articles/PMC9409299/ /pubmed/36012624 http://dx.doi.org/10.3390/ijms23169370 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yan, Heqin Zheng, Wei Wang, Yong Wu, Yougen Yu, Jing Xia, Pengguo Integrative Metabolome and Transcriptome Analysis Reveals the Regulatory Network of Flavonoid Biosynthesis in Response to MeJA in Camellia vietnamensis Huang |
title | Integrative Metabolome and Transcriptome Analysis Reveals the Regulatory Network of Flavonoid Biosynthesis in Response to MeJA in Camellia
vietnamensis Huang |
title_full | Integrative Metabolome and Transcriptome Analysis Reveals the Regulatory Network of Flavonoid Biosynthesis in Response to MeJA in Camellia
vietnamensis Huang |
title_fullStr | Integrative Metabolome and Transcriptome Analysis Reveals the Regulatory Network of Flavonoid Biosynthesis in Response to MeJA in Camellia
vietnamensis Huang |
title_full_unstemmed | Integrative Metabolome and Transcriptome Analysis Reveals the Regulatory Network of Flavonoid Biosynthesis in Response to MeJA in Camellia
vietnamensis Huang |
title_short | Integrative Metabolome and Transcriptome Analysis Reveals the Regulatory Network of Flavonoid Biosynthesis in Response to MeJA in Camellia
vietnamensis Huang |
title_sort | integrative metabolome and transcriptome analysis reveals the regulatory network of flavonoid biosynthesis in response to meja in camellia
vietnamensis huang |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409299/ https://www.ncbi.nlm.nih.gov/pubmed/36012624 http://dx.doi.org/10.3390/ijms23169370 |
work_keys_str_mv | AT yanheqin integrativemetabolomeandtranscriptomeanalysisrevealstheregulatorynetworkofflavonoidbiosynthesisinresponsetomejaincamelliavietnamensishuang AT zhengwei integrativemetabolomeandtranscriptomeanalysisrevealstheregulatorynetworkofflavonoidbiosynthesisinresponsetomejaincamelliavietnamensishuang AT wangyong integrativemetabolomeandtranscriptomeanalysisrevealstheregulatorynetworkofflavonoidbiosynthesisinresponsetomejaincamelliavietnamensishuang AT wuyougen integrativemetabolomeandtranscriptomeanalysisrevealstheregulatorynetworkofflavonoidbiosynthesisinresponsetomejaincamelliavietnamensishuang AT yujing integrativemetabolomeandtranscriptomeanalysisrevealstheregulatorynetworkofflavonoidbiosynthesisinresponsetomejaincamelliavietnamensishuang AT xiapengguo integrativemetabolomeandtranscriptomeanalysisrevealstheregulatorynetworkofflavonoidbiosynthesisinresponsetomejaincamelliavietnamensishuang |