Analysis of Ser/Thr Kinase HASPIN-Interacting Proteins in the Spermatids

HASPIN is predominantly expressed in spermatids, and plays an important role in cell division in somatic and meiotic cells through histone H3 phosphorylation. The literature published to date has suggested that HASPIN may play multiple roles in cells. Here, 10 gene products from the mouse testis cDN...

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Autores principales: Maeda, Naoko, Tsuchida, Junji, Nishimune, Yoshitake, Tanaka, Hiromitsu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409403/
https://www.ncbi.nlm.nih.gov/pubmed/36012324
http://dx.doi.org/10.3390/ijms23169060
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author Maeda, Naoko
Tsuchida, Junji
Nishimune, Yoshitake
Tanaka, Hiromitsu
author_facet Maeda, Naoko
Tsuchida, Junji
Nishimune, Yoshitake
Tanaka, Hiromitsu
author_sort Maeda, Naoko
collection PubMed
description HASPIN is predominantly expressed in spermatids, and plays an important role in cell division in somatic and meiotic cells through histone H3 phosphorylation. The literature published to date has suggested that HASPIN may play multiple roles in cells. Here, 10 gene products from the mouse testis cDNA library that interact with HASPIN were isolated using the two-hybrid system. Among them, CENPJ/CPAP, KPNA6/importin alpha 6, and C1QBP/HABP1 were analyzed in detail for their interactions with HASPIN, with HASPIN phosphorylated C1QBP as the substrate. The results indicated that HASPIN is involved in spermatogenesis through the phosphorylation of C1QBP in spermatids, and also may be involved in the formation of centrosomes.
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spelling pubmed-94094032022-08-26 Analysis of Ser/Thr Kinase HASPIN-Interacting Proteins in the Spermatids Maeda, Naoko Tsuchida, Junji Nishimune, Yoshitake Tanaka, Hiromitsu Int J Mol Sci Article HASPIN is predominantly expressed in spermatids, and plays an important role in cell division in somatic and meiotic cells through histone H3 phosphorylation. The literature published to date has suggested that HASPIN may play multiple roles in cells. Here, 10 gene products from the mouse testis cDNA library that interact with HASPIN were isolated using the two-hybrid system. Among them, CENPJ/CPAP, KPNA6/importin alpha 6, and C1QBP/HABP1 were analyzed in detail for their interactions with HASPIN, with HASPIN phosphorylated C1QBP as the substrate. The results indicated that HASPIN is involved in spermatogenesis through the phosphorylation of C1QBP in spermatids, and also may be involved in the formation of centrosomes. MDPI 2022-08-13 /pmc/articles/PMC9409403/ /pubmed/36012324 http://dx.doi.org/10.3390/ijms23169060 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Maeda, Naoko
Tsuchida, Junji
Nishimune, Yoshitake
Tanaka, Hiromitsu
Analysis of Ser/Thr Kinase HASPIN-Interacting Proteins in the Spermatids
title Analysis of Ser/Thr Kinase HASPIN-Interacting Proteins in the Spermatids
title_full Analysis of Ser/Thr Kinase HASPIN-Interacting Proteins in the Spermatids
title_fullStr Analysis of Ser/Thr Kinase HASPIN-Interacting Proteins in the Spermatids
title_full_unstemmed Analysis of Ser/Thr Kinase HASPIN-Interacting Proteins in the Spermatids
title_short Analysis of Ser/Thr Kinase HASPIN-Interacting Proteins in the Spermatids
title_sort analysis of ser/thr kinase haspin-interacting proteins in the spermatids
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409403/
https://www.ncbi.nlm.nih.gov/pubmed/36012324
http://dx.doi.org/10.3390/ijms23169060
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AT tanakahiromitsu analysisofserthrkinasehaspininteractingproteinsinthespermatids

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