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The Activity of Chelidonium majus L. Latex and Its Components on HPV Reveal Insights into the Antiviral Molecular Mechanism

Yellow-orange latex of Chelidonium majus L. has been used in folk medicine as a therapeutic agent against warts and other visible symptoms of human papillomavirus (HPV) infections for centuries. The observed antiviral and antitumor properties of C. majus latex are often attributed to alkaloids conta...

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Autores principales: Musidlak, Oskar, Warowicka, Alicja, Broniarczyk, Justyna, Adamczyk, Damian, Goździcka-Józefiak, Anna, Nawrot, Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409487/
https://www.ncbi.nlm.nih.gov/pubmed/36012505
http://dx.doi.org/10.3390/ijms23169241
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author Musidlak, Oskar
Warowicka, Alicja
Broniarczyk, Justyna
Adamczyk, Damian
Goździcka-Józefiak, Anna
Nawrot, Robert
author_facet Musidlak, Oskar
Warowicka, Alicja
Broniarczyk, Justyna
Adamczyk, Damian
Goździcka-Józefiak, Anna
Nawrot, Robert
author_sort Musidlak, Oskar
collection PubMed
description Yellow-orange latex of Chelidonium majus L. has been used in folk medicine as a therapeutic agent against warts and other visible symptoms of human papillomavirus (HPV) infections for centuries. The observed antiviral and antitumor properties of C. majus latex are often attributed to alkaloids contained therein, but recent studies indicate that latex proteins may also play an important role in its pharmacological activities. Therefore, the aim of the study was to investigate the effect of the crude C. majus latex and its protein and alkaloid-rich fractions on different stages of the HPV replication cycle. The results showed that the latex components, such as alkaloids and proteins, decrease HPV infectivity and inhibit the expression of viral oncogenes (E6, E7) on mRNA and protein levels. However, the crude latex and its fractions do not affect the stability of structural proteins in HPV pseudovirions and they do not inhibit the virus from attaching to the cell surface. In addition, the protein fraction causes increased TNFα secretion, which may indicate the induction of an inflammatory response. These findings indicate that the antiviral properties of C. majus latex arise both from alkaloids and proteins contained therein, acting on different stages of the viral replication cycle.
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spelling pubmed-94094872022-08-26 The Activity of Chelidonium majus L. Latex and Its Components on HPV Reveal Insights into the Antiviral Molecular Mechanism Musidlak, Oskar Warowicka, Alicja Broniarczyk, Justyna Adamczyk, Damian Goździcka-Józefiak, Anna Nawrot, Robert Int J Mol Sci Article Yellow-orange latex of Chelidonium majus L. has been used in folk medicine as a therapeutic agent against warts and other visible symptoms of human papillomavirus (HPV) infections for centuries. The observed antiviral and antitumor properties of C. majus latex are often attributed to alkaloids contained therein, but recent studies indicate that latex proteins may also play an important role in its pharmacological activities. Therefore, the aim of the study was to investigate the effect of the crude C. majus latex and its protein and alkaloid-rich fractions on different stages of the HPV replication cycle. The results showed that the latex components, such as alkaloids and proteins, decrease HPV infectivity and inhibit the expression of viral oncogenes (E6, E7) on mRNA and protein levels. However, the crude latex and its fractions do not affect the stability of structural proteins in HPV pseudovirions and they do not inhibit the virus from attaching to the cell surface. In addition, the protein fraction causes increased TNFα secretion, which may indicate the induction of an inflammatory response. These findings indicate that the antiviral properties of C. majus latex arise both from alkaloids and proteins contained therein, acting on different stages of the viral replication cycle. MDPI 2022-08-17 /pmc/articles/PMC9409487/ /pubmed/36012505 http://dx.doi.org/10.3390/ijms23169241 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Musidlak, Oskar
Warowicka, Alicja
Broniarczyk, Justyna
Adamczyk, Damian
Goździcka-Józefiak, Anna
Nawrot, Robert
The Activity of Chelidonium majus L. Latex and Its Components on HPV Reveal Insights into the Antiviral Molecular Mechanism
title The Activity of Chelidonium majus L. Latex and Its Components on HPV Reveal Insights into the Antiviral Molecular Mechanism
title_full The Activity of Chelidonium majus L. Latex and Its Components on HPV Reveal Insights into the Antiviral Molecular Mechanism
title_fullStr The Activity of Chelidonium majus L. Latex and Its Components on HPV Reveal Insights into the Antiviral Molecular Mechanism
title_full_unstemmed The Activity of Chelidonium majus L. Latex and Its Components on HPV Reveal Insights into the Antiviral Molecular Mechanism
title_short The Activity of Chelidonium majus L. Latex and Its Components on HPV Reveal Insights into the Antiviral Molecular Mechanism
title_sort activity of chelidonium majus l. latex and its components on hpv reveal insights into the antiviral molecular mechanism
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409487/
https://www.ncbi.nlm.nih.gov/pubmed/36012505
http://dx.doi.org/10.3390/ijms23169241
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