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Functions of block of proliferation 1 during anterior development in Xenopus laevis

Block of proliferation 1 (Bop1) is a nucleolar protein known to be necessary for the assembly of the 60S subunit of ribosomes. Here, we show a specific bop1 expression in the developing anterior tissue of the South African clawed frog Xenopus laevis. Morpholino oligonucleotide-mediated knockdown app...

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Autores principales: Gärtner, Corinna, Meßmer, Annika, Dietmann, Petra, Kühl, Michael, Kühl, Susanne J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409556/
https://www.ncbi.nlm.nih.gov/pubmed/36007075
http://dx.doi.org/10.1371/journal.pone.0273507
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author Gärtner, Corinna
Meßmer, Annika
Dietmann, Petra
Kühl, Michael
Kühl, Susanne J.
author_facet Gärtner, Corinna
Meßmer, Annika
Dietmann, Petra
Kühl, Michael
Kühl, Susanne J.
author_sort Gärtner, Corinna
collection PubMed
description Block of proliferation 1 (Bop1) is a nucleolar protein known to be necessary for the assembly of the 60S subunit of ribosomes. Here, we show a specific bop1 expression in the developing anterior tissue of the South African clawed frog Xenopus laevis. Morpholino oligonucleotide-mediated knockdown approaches demonstrated that Bop1 is required for proper development of the cranial cartilage, brain, and the eyes. Furthermore, we show that bop1 knockdown leads to impaired retinal lamination with disorganized cell layers. Expression of neural crest-, brain-, and eye-specific marker genes was disturbed. Apoptotic and proliferative processes, which are known to be affected during ribosomal biogenesis defects, are not hindered upon bop1 knockdown. Because early Xenopus embryos contain a large store of maternal ribosomes, we considered if Bop1 might have a role independent of de novo ribosomal biogenesis. At early embryonic stages, pax6 expression was strongly reduced in bop1 morphants and synergy experiments indicate a common signaling pathway of the two molecules, Bop1 and Pax6. Our studies imply a novel function of Bop1 independent of ribosomal biogenesis.
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spelling pubmed-94095562022-08-26 Functions of block of proliferation 1 during anterior development in Xenopus laevis Gärtner, Corinna Meßmer, Annika Dietmann, Petra Kühl, Michael Kühl, Susanne J. PLoS One Research Article Block of proliferation 1 (Bop1) is a nucleolar protein known to be necessary for the assembly of the 60S subunit of ribosomes. Here, we show a specific bop1 expression in the developing anterior tissue of the South African clawed frog Xenopus laevis. Morpholino oligonucleotide-mediated knockdown approaches demonstrated that Bop1 is required for proper development of the cranial cartilage, brain, and the eyes. Furthermore, we show that bop1 knockdown leads to impaired retinal lamination with disorganized cell layers. Expression of neural crest-, brain-, and eye-specific marker genes was disturbed. Apoptotic and proliferative processes, which are known to be affected during ribosomal biogenesis defects, are not hindered upon bop1 knockdown. Because early Xenopus embryos contain a large store of maternal ribosomes, we considered if Bop1 might have a role independent of de novo ribosomal biogenesis. At early embryonic stages, pax6 expression was strongly reduced in bop1 morphants and synergy experiments indicate a common signaling pathway of the two molecules, Bop1 and Pax6. Our studies imply a novel function of Bop1 independent of ribosomal biogenesis. Public Library of Science 2022-08-25 /pmc/articles/PMC9409556/ /pubmed/36007075 http://dx.doi.org/10.1371/journal.pone.0273507 Text en © 2022 Gärtner et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Gärtner, Corinna
Meßmer, Annika
Dietmann, Petra
Kühl, Michael
Kühl, Susanne J.
Functions of block of proliferation 1 during anterior development in Xenopus laevis
title Functions of block of proliferation 1 during anterior development in Xenopus laevis
title_full Functions of block of proliferation 1 during anterior development in Xenopus laevis
title_fullStr Functions of block of proliferation 1 during anterior development in Xenopus laevis
title_full_unstemmed Functions of block of proliferation 1 during anterior development in Xenopus laevis
title_short Functions of block of proliferation 1 during anterior development in Xenopus laevis
title_sort functions of block of proliferation 1 during anterior development in xenopus laevis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409556/
https://www.ncbi.nlm.nih.gov/pubmed/36007075
http://dx.doi.org/10.1371/journal.pone.0273507
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