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Microbial Diversity and Community Composition of Duodenum Microbiota of High and Low Egg-Yielding Taihang Chickens Identified Using 16S rRNA Amplicon Sequencing

The duodenum is an important digestive organ for poultry and houses a variety of microbes that help chickens to enhance nutrient absorption and improve production. To evaluate the characteristic of gut microbiome, duodenum content samples from 42-week-old native Taihang chickens with high (H) and lo...

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Autores principales: Han, Haiyin, Sun, Yingjie, Fan, Yekai, Zhang, Hui, Yang, Junqi, Chi, Runqing, Gao, Yahui, Liu, Jiannan, Li, Kaiyang, Li, Wenting, Liu, Yufang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409686/
https://www.ncbi.nlm.nih.gov/pubmed/36013441
http://dx.doi.org/10.3390/life12081262
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author Han, Haiyin
Sun, Yingjie
Fan, Yekai
Zhang, Hui
Yang, Junqi
Chi, Runqing
Gao, Yahui
Liu, Jiannan
Li, Kaiyang
Li, Wenting
Liu, Yufang
author_facet Han, Haiyin
Sun, Yingjie
Fan, Yekai
Zhang, Hui
Yang, Junqi
Chi, Runqing
Gao, Yahui
Liu, Jiannan
Li, Kaiyang
Li, Wenting
Liu, Yufang
author_sort Han, Haiyin
collection PubMed
description The duodenum is an important digestive organ for poultry and houses a variety of microbes that help chickens to enhance nutrient absorption and improve production. To evaluate the characteristic of gut microbiome, duodenum content samples from 42-week-old native Taihang chickens with high (H) and low (L) egg-yielding were collected for 16S rRNA amplicon sequencing analysis. Consequently, 1,361,341 sequences were clustered into 2055 OTUs, with percentages of affiliation of 96.50 and 57.30% at phylum and genus levels. Firmicutes, Proteobacteria, Cyanobacteria and Bacteroidetes were the dominant phylum, with a lower ratio of Firmicutes/Bacteroidetes in H group than in L group (p < 0.05). At genus level, overrepresentation of Bacteroides, Faecalibacterim, and Enterococcus and underrepresentation of Romboutsia were found in H group. No significant difference in overall diversity of microbiota was observed between two groups. LEFSe analysis revealed Enterococcus was significantly enriched in H group. Importantly, Enterococcus and Lactobacillus were negatively correlated. Functional prediction analysis showed the proportion of microbiota involved in the metabolism process was the highest and enriched in H group. Differences in microbiota composition between the two groups, which may be related to intestinal function difference, also provide promising biomarkers for improving laying hen production.
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spelling pubmed-94096862022-08-26 Microbial Diversity and Community Composition of Duodenum Microbiota of High and Low Egg-Yielding Taihang Chickens Identified Using 16S rRNA Amplicon Sequencing Han, Haiyin Sun, Yingjie Fan, Yekai Zhang, Hui Yang, Junqi Chi, Runqing Gao, Yahui Liu, Jiannan Li, Kaiyang Li, Wenting Liu, Yufang Life (Basel) Article The duodenum is an important digestive organ for poultry and houses a variety of microbes that help chickens to enhance nutrient absorption and improve production. To evaluate the characteristic of gut microbiome, duodenum content samples from 42-week-old native Taihang chickens with high (H) and low (L) egg-yielding were collected for 16S rRNA amplicon sequencing analysis. Consequently, 1,361,341 sequences were clustered into 2055 OTUs, with percentages of affiliation of 96.50 and 57.30% at phylum and genus levels. Firmicutes, Proteobacteria, Cyanobacteria and Bacteroidetes were the dominant phylum, with a lower ratio of Firmicutes/Bacteroidetes in H group than in L group (p < 0.05). At genus level, overrepresentation of Bacteroides, Faecalibacterim, and Enterococcus and underrepresentation of Romboutsia were found in H group. No significant difference in overall diversity of microbiota was observed between two groups. LEFSe analysis revealed Enterococcus was significantly enriched in H group. Importantly, Enterococcus and Lactobacillus were negatively correlated. Functional prediction analysis showed the proportion of microbiota involved in the metabolism process was the highest and enriched in H group. Differences in microbiota composition between the two groups, which may be related to intestinal function difference, also provide promising biomarkers for improving laying hen production. MDPI 2022-08-18 /pmc/articles/PMC9409686/ /pubmed/36013441 http://dx.doi.org/10.3390/life12081262 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Han, Haiyin
Sun, Yingjie
Fan, Yekai
Zhang, Hui
Yang, Junqi
Chi, Runqing
Gao, Yahui
Liu, Jiannan
Li, Kaiyang
Li, Wenting
Liu, Yufang
Microbial Diversity and Community Composition of Duodenum Microbiota of High and Low Egg-Yielding Taihang Chickens Identified Using 16S rRNA Amplicon Sequencing
title Microbial Diversity and Community Composition of Duodenum Microbiota of High and Low Egg-Yielding Taihang Chickens Identified Using 16S rRNA Amplicon Sequencing
title_full Microbial Diversity and Community Composition of Duodenum Microbiota of High and Low Egg-Yielding Taihang Chickens Identified Using 16S rRNA Amplicon Sequencing
title_fullStr Microbial Diversity and Community Composition of Duodenum Microbiota of High and Low Egg-Yielding Taihang Chickens Identified Using 16S rRNA Amplicon Sequencing
title_full_unstemmed Microbial Diversity and Community Composition of Duodenum Microbiota of High and Low Egg-Yielding Taihang Chickens Identified Using 16S rRNA Amplicon Sequencing
title_short Microbial Diversity and Community Composition of Duodenum Microbiota of High and Low Egg-Yielding Taihang Chickens Identified Using 16S rRNA Amplicon Sequencing
title_sort microbial diversity and community composition of duodenum microbiota of high and low egg-yielding taihang chickens identified using 16s rrna amplicon sequencing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409686/
https://www.ncbi.nlm.nih.gov/pubmed/36013441
http://dx.doi.org/10.3390/life12081262
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