Cargando…

Characterization of Bacterial Communities from the Surface and Adjacent Bottom Layers of Water in the Billings Reservoir

Here, we describe the bacterial diversity and physicochemical properties in freshwater samples from the surface and bottom layers of the Billings Reservoir, the largest open-air storage ecosystem in the São Paulo (Brazil) metropolitan area. Forty-four samples (22 from the surface and 22 from the bot...

Descripción completa

Detalles Bibliográficos
Autores principales: Marcondes, Marta Angela, Nascimento, Andrezza, Pessôa, Rodrigo, Victor, Jefferson Russo, Duarte, Alberto José da Silva, Clissa, Patricia Bianca, Sanabani, Sabri Saeed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409723/
https://www.ncbi.nlm.nih.gov/pubmed/36013459
http://dx.doi.org/10.3390/life12081280
_version_ 1784774920948940800
author Marcondes, Marta Angela
Nascimento, Andrezza
Pessôa, Rodrigo
Victor, Jefferson Russo
Duarte, Alberto José da Silva
Clissa, Patricia Bianca
Sanabani, Sabri Saeed
author_facet Marcondes, Marta Angela
Nascimento, Andrezza
Pessôa, Rodrigo
Victor, Jefferson Russo
Duarte, Alberto José da Silva
Clissa, Patricia Bianca
Sanabani, Sabri Saeed
author_sort Marcondes, Marta Angela
collection PubMed
description Here, we describe the bacterial diversity and physicochemical properties in freshwater samples from the surface and bottom layers of the Billings Reservoir, the largest open-air storage ecosystem in the São Paulo (Brazil) metropolitan area. Forty-four samples (22 from the surface and 22 from the bottom layers) were characterized based on 16S rRNA gene analysis using Illumina MiSeq. Taxonomical composition revealed an abundance of the Cyanobacteria phylum, followed by Proteobacteria, which were grouped into 1903 and 2689 different genera in the surface and the deep-water layers, respectively. Chroobacteria, Actinobacteria, Betaproteobacteria, and Alphaproteobacteria were the most dominant classes. The Shannon diversity index was in the range of 2.3–5.39 and 4.04–6.86 in the surface and bottom layers, respectively. Flavobacterium was the most predominant pathogenic genus. Temperature and phosphorus concentrations were among the most influential factors in shaping the microbial communities of both layers. Predictive functional analysis suggests that the reservoir is enriched in motility genes involved in flagellar assembly. The overall results provide new information on the diversity composition, ecological function, and health risks of the bacterial community detected in the Billings freshwater reservoir. The broad bacterial diversity indicates that the bacterioplankton communities in the reservoir were involved in multiple essential environmental processes.
format Online
Article
Text
id pubmed-9409723
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-94097232022-08-26 Characterization of Bacterial Communities from the Surface and Adjacent Bottom Layers of Water in the Billings Reservoir Marcondes, Marta Angela Nascimento, Andrezza Pessôa, Rodrigo Victor, Jefferson Russo Duarte, Alberto José da Silva Clissa, Patricia Bianca Sanabani, Sabri Saeed Life (Basel) Article Here, we describe the bacterial diversity and physicochemical properties in freshwater samples from the surface and bottom layers of the Billings Reservoir, the largest open-air storage ecosystem in the São Paulo (Brazil) metropolitan area. Forty-four samples (22 from the surface and 22 from the bottom layers) were characterized based on 16S rRNA gene analysis using Illumina MiSeq. Taxonomical composition revealed an abundance of the Cyanobacteria phylum, followed by Proteobacteria, which were grouped into 1903 and 2689 different genera in the surface and the deep-water layers, respectively. Chroobacteria, Actinobacteria, Betaproteobacteria, and Alphaproteobacteria were the most dominant classes. The Shannon diversity index was in the range of 2.3–5.39 and 4.04–6.86 in the surface and bottom layers, respectively. Flavobacterium was the most predominant pathogenic genus. Temperature and phosphorus concentrations were among the most influential factors in shaping the microbial communities of both layers. Predictive functional analysis suggests that the reservoir is enriched in motility genes involved in flagellar assembly. The overall results provide new information on the diversity composition, ecological function, and health risks of the bacterial community detected in the Billings freshwater reservoir. The broad bacterial diversity indicates that the bacterioplankton communities in the reservoir were involved in multiple essential environmental processes. MDPI 2022-08-22 /pmc/articles/PMC9409723/ /pubmed/36013459 http://dx.doi.org/10.3390/life12081280 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Marcondes, Marta Angela
Nascimento, Andrezza
Pessôa, Rodrigo
Victor, Jefferson Russo
Duarte, Alberto José da Silva
Clissa, Patricia Bianca
Sanabani, Sabri Saeed
Characterization of Bacterial Communities from the Surface and Adjacent Bottom Layers of Water in the Billings Reservoir
title Characterization of Bacterial Communities from the Surface and Adjacent Bottom Layers of Water in the Billings Reservoir
title_full Characterization of Bacterial Communities from the Surface and Adjacent Bottom Layers of Water in the Billings Reservoir
title_fullStr Characterization of Bacterial Communities from the Surface and Adjacent Bottom Layers of Water in the Billings Reservoir
title_full_unstemmed Characterization of Bacterial Communities from the Surface and Adjacent Bottom Layers of Water in the Billings Reservoir
title_short Characterization of Bacterial Communities from the Surface and Adjacent Bottom Layers of Water in the Billings Reservoir
title_sort characterization of bacterial communities from the surface and adjacent bottom layers of water in the billings reservoir
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409723/
https://www.ncbi.nlm.nih.gov/pubmed/36013459
http://dx.doi.org/10.3390/life12081280
work_keys_str_mv AT marcondesmartaangela characterizationofbacterialcommunitiesfromthesurfaceandadjacentbottomlayersofwaterinthebillingsreservoir
AT nascimentoandrezza characterizationofbacterialcommunitiesfromthesurfaceandadjacentbottomlayersofwaterinthebillingsreservoir
AT pessoarodrigo characterizationofbacterialcommunitiesfromthesurfaceandadjacentbottomlayersofwaterinthebillingsreservoir
AT victorjeffersonrusso characterizationofbacterialcommunitiesfromthesurfaceandadjacentbottomlayersofwaterinthebillingsreservoir
AT duartealbertojosedasilva characterizationofbacterialcommunitiesfromthesurfaceandadjacentbottomlayersofwaterinthebillingsreservoir
AT clissapatriciabianca characterizationofbacterialcommunitiesfromthesurfaceandadjacentbottomlayersofwaterinthebillingsreservoir
AT sanabanisabrisaeed characterizationofbacterialcommunitiesfromthesurfaceandadjacentbottomlayersofwaterinthebillingsreservoir