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Characterization of Bacterial Communities from the Surface and Adjacent Bottom Layers of Water in the Billings Reservoir
Here, we describe the bacterial diversity and physicochemical properties in freshwater samples from the surface and bottom layers of the Billings Reservoir, the largest open-air storage ecosystem in the São Paulo (Brazil) metropolitan area. Forty-four samples (22 from the surface and 22 from the bot...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409723/ https://www.ncbi.nlm.nih.gov/pubmed/36013459 http://dx.doi.org/10.3390/life12081280 |
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author | Marcondes, Marta Angela Nascimento, Andrezza Pessôa, Rodrigo Victor, Jefferson Russo Duarte, Alberto José da Silva Clissa, Patricia Bianca Sanabani, Sabri Saeed |
author_facet | Marcondes, Marta Angela Nascimento, Andrezza Pessôa, Rodrigo Victor, Jefferson Russo Duarte, Alberto José da Silva Clissa, Patricia Bianca Sanabani, Sabri Saeed |
author_sort | Marcondes, Marta Angela |
collection | PubMed |
description | Here, we describe the bacterial diversity and physicochemical properties in freshwater samples from the surface and bottom layers of the Billings Reservoir, the largest open-air storage ecosystem in the São Paulo (Brazil) metropolitan area. Forty-four samples (22 from the surface and 22 from the bottom layers) were characterized based on 16S rRNA gene analysis using Illumina MiSeq. Taxonomical composition revealed an abundance of the Cyanobacteria phylum, followed by Proteobacteria, which were grouped into 1903 and 2689 different genera in the surface and the deep-water layers, respectively. Chroobacteria, Actinobacteria, Betaproteobacteria, and Alphaproteobacteria were the most dominant classes. The Shannon diversity index was in the range of 2.3–5.39 and 4.04–6.86 in the surface and bottom layers, respectively. Flavobacterium was the most predominant pathogenic genus. Temperature and phosphorus concentrations were among the most influential factors in shaping the microbial communities of both layers. Predictive functional analysis suggests that the reservoir is enriched in motility genes involved in flagellar assembly. The overall results provide new information on the diversity composition, ecological function, and health risks of the bacterial community detected in the Billings freshwater reservoir. The broad bacterial diversity indicates that the bacterioplankton communities in the reservoir were involved in multiple essential environmental processes. |
format | Online Article Text |
id | pubmed-9409723 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94097232022-08-26 Characterization of Bacterial Communities from the Surface and Adjacent Bottom Layers of Water in the Billings Reservoir Marcondes, Marta Angela Nascimento, Andrezza Pessôa, Rodrigo Victor, Jefferson Russo Duarte, Alberto José da Silva Clissa, Patricia Bianca Sanabani, Sabri Saeed Life (Basel) Article Here, we describe the bacterial diversity and physicochemical properties in freshwater samples from the surface and bottom layers of the Billings Reservoir, the largest open-air storage ecosystem in the São Paulo (Brazil) metropolitan area. Forty-four samples (22 from the surface and 22 from the bottom layers) were characterized based on 16S rRNA gene analysis using Illumina MiSeq. Taxonomical composition revealed an abundance of the Cyanobacteria phylum, followed by Proteobacteria, which were grouped into 1903 and 2689 different genera in the surface and the deep-water layers, respectively. Chroobacteria, Actinobacteria, Betaproteobacteria, and Alphaproteobacteria were the most dominant classes. The Shannon diversity index was in the range of 2.3–5.39 and 4.04–6.86 in the surface and bottom layers, respectively. Flavobacterium was the most predominant pathogenic genus. Temperature and phosphorus concentrations were among the most influential factors in shaping the microbial communities of both layers. Predictive functional analysis suggests that the reservoir is enriched in motility genes involved in flagellar assembly. The overall results provide new information on the diversity composition, ecological function, and health risks of the bacterial community detected in the Billings freshwater reservoir. The broad bacterial diversity indicates that the bacterioplankton communities in the reservoir were involved in multiple essential environmental processes. MDPI 2022-08-22 /pmc/articles/PMC9409723/ /pubmed/36013459 http://dx.doi.org/10.3390/life12081280 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Marcondes, Marta Angela Nascimento, Andrezza Pessôa, Rodrigo Victor, Jefferson Russo Duarte, Alberto José da Silva Clissa, Patricia Bianca Sanabani, Sabri Saeed Characterization of Bacterial Communities from the Surface and Adjacent Bottom Layers of Water in the Billings Reservoir |
title | Characterization of Bacterial Communities from the Surface and Adjacent Bottom Layers of Water in the Billings Reservoir |
title_full | Characterization of Bacterial Communities from the Surface and Adjacent Bottom Layers of Water in the Billings Reservoir |
title_fullStr | Characterization of Bacterial Communities from the Surface and Adjacent Bottom Layers of Water in the Billings Reservoir |
title_full_unstemmed | Characterization of Bacterial Communities from the Surface and Adjacent Bottom Layers of Water in the Billings Reservoir |
title_short | Characterization of Bacterial Communities from the Surface and Adjacent Bottom Layers of Water in the Billings Reservoir |
title_sort | characterization of bacterial communities from the surface and adjacent bottom layers of water in the billings reservoir |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409723/ https://www.ncbi.nlm.nih.gov/pubmed/36013459 http://dx.doi.org/10.3390/life12081280 |
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