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Genetic Diversity Analysis based on the Virulence, Physiology and Regional Variability in Different Isolates of Powdery Mildew in Pea

Powdery mildew is an omnipresent disease that reduces the yield and quality of pea crops (Pisum sativum L.). To examine the powdery mildew pathogen’s morphological, molecular, and genetic diversity, we collected samples of powdery mildew-affected pea crops from ten distinct locations in the Nilgiris...

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Autores principales: Seethapathy, Parthasarathy, Sankaralingam, Subbiah, Pandita, Deepu, Pandita, Anu, Loganathan, Kousalya, Wani, Shabir Hussain, El-Ansary, Diaa O., Sharma, Hanoor, Casini, Ryan, Mahmoud, Eman A., Elansary, Hosam O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409743/
https://www.ncbi.nlm.nih.gov/pubmed/36012787
http://dx.doi.org/10.3390/jof8080798
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author Seethapathy, Parthasarathy
Sankaralingam, Subbiah
Pandita, Deepu
Pandita, Anu
Loganathan, Kousalya
Wani, Shabir Hussain
El-Ansary, Diaa O.
Sharma, Hanoor
Casini, Ryan
Mahmoud, Eman A.
Elansary, Hosam O.
author_facet Seethapathy, Parthasarathy
Sankaralingam, Subbiah
Pandita, Deepu
Pandita, Anu
Loganathan, Kousalya
Wani, Shabir Hussain
El-Ansary, Diaa O.
Sharma, Hanoor
Casini, Ryan
Mahmoud, Eman A.
Elansary, Hosam O.
author_sort Seethapathy, Parthasarathy
collection PubMed
description Powdery mildew is an omnipresent disease that reduces the yield and quality of pea crops (Pisum sativum L.). To examine the powdery mildew pathogen’s morphological, molecular, and genetic diversity, we collected samples of powdery mildew-affected pea crops from ten distinct locations in the Nilgiris district of Tamil Nadu, India. The pathogen Erysiphe pisi was identified morphologically based on anamorphic characters. Molecular identification of E. pisi isolates was befitted by targeting the internal transcribed spacer (ITS) region of rDNA and specific primers of powdery mildew fungi. The genetic variation between ten different E. pisi isolates collected from topographically distinct mountainous areas was studied using random amplified polymorphic (RAPD). Based on its morphological characteristics, the powdery mildew fungus presented high similarities to E. pisi. Molecular characterization of the ITS rDNA of E. pisi produced 650 bp nucleotides, PMITS (powdery mildew-internal transcribed region) primers produced 700 bp nucleotides, and an Erysiphe specific ITS primer pair amplified and synthesized 560 bp nucleotides. According to the findings, the collected E. pisi strains exhibited a low level of genetic diversity and only a slight differential in virulence on the host. In the study, E. pisi isolates from Anumapuram, Emerald Valley, Indira Nagar, and Thuneri showed a greater disease incidence in the natural field conditions and shared the same genetic lineage with other isolates in UPGMA hierarchical cluster analysis based on RAPD markers. There was no evidence of a link between the occurrence of the disease and these grouped populations.
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spelling pubmed-94097432022-08-26 Genetic Diversity Analysis based on the Virulence, Physiology and Regional Variability in Different Isolates of Powdery Mildew in Pea Seethapathy, Parthasarathy Sankaralingam, Subbiah Pandita, Deepu Pandita, Anu Loganathan, Kousalya Wani, Shabir Hussain El-Ansary, Diaa O. Sharma, Hanoor Casini, Ryan Mahmoud, Eman A. Elansary, Hosam O. J Fungi (Basel) Article Powdery mildew is an omnipresent disease that reduces the yield and quality of pea crops (Pisum sativum L.). To examine the powdery mildew pathogen’s morphological, molecular, and genetic diversity, we collected samples of powdery mildew-affected pea crops from ten distinct locations in the Nilgiris district of Tamil Nadu, India. The pathogen Erysiphe pisi was identified morphologically based on anamorphic characters. Molecular identification of E. pisi isolates was befitted by targeting the internal transcribed spacer (ITS) region of rDNA and specific primers of powdery mildew fungi. The genetic variation between ten different E. pisi isolates collected from topographically distinct mountainous areas was studied using random amplified polymorphic (RAPD). Based on its morphological characteristics, the powdery mildew fungus presented high similarities to E. pisi. Molecular characterization of the ITS rDNA of E. pisi produced 650 bp nucleotides, PMITS (powdery mildew-internal transcribed region) primers produced 700 bp nucleotides, and an Erysiphe specific ITS primer pair amplified and synthesized 560 bp nucleotides. According to the findings, the collected E. pisi strains exhibited a low level of genetic diversity and only a slight differential in virulence on the host. In the study, E. pisi isolates from Anumapuram, Emerald Valley, Indira Nagar, and Thuneri showed a greater disease incidence in the natural field conditions and shared the same genetic lineage with other isolates in UPGMA hierarchical cluster analysis based on RAPD markers. There was no evidence of a link between the occurrence of the disease and these grouped populations. MDPI 2022-07-29 /pmc/articles/PMC9409743/ /pubmed/36012787 http://dx.doi.org/10.3390/jof8080798 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Seethapathy, Parthasarathy
Sankaralingam, Subbiah
Pandita, Deepu
Pandita, Anu
Loganathan, Kousalya
Wani, Shabir Hussain
El-Ansary, Diaa O.
Sharma, Hanoor
Casini, Ryan
Mahmoud, Eman A.
Elansary, Hosam O.
Genetic Diversity Analysis based on the Virulence, Physiology and Regional Variability in Different Isolates of Powdery Mildew in Pea
title Genetic Diversity Analysis based on the Virulence, Physiology and Regional Variability in Different Isolates of Powdery Mildew in Pea
title_full Genetic Diversity Analysis based on the Virulence, Physiology and Regional Variability in Different Isolates of Powdery Mildew in Pea
title_fullStr Genetic Diversity Analysis based on the Virulence, Physiology and Regional Variability in Different Isolates of Powdery Mildew in Pea
title_full_unstemmed Genetic Diversity Analysis based on the Virulence, Physiology and Regional Variability in Different Isolates of Powdery Mildew in Pea
title_short Genetic Diversity Analysis based on the Virulence, Physiology and Regional Variability in Different Isolates of Powdery Mildew in Pea
title_sort genetic diversity analysis based on the virulence, physiology and regional variability in different isolates of powdery mildew in pea
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409743/
https://www.ncbi.nlm.nih.gov/pubmed/36012787
http://dx.doi.org/10.3390/jof8080798
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