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Inhibitory Effect of Bacillus licheniformis Strains Isolated from Canine Oral Cavity

Bacillus licheniformis is used in a broad spectrum of areas, including some probiotic preparations for human and veterinary health. Moreover, B. licheniformis strains are known producers of various bioactive substances with antimicrobial and antibiofilm effects. In searching for new potentially bene...

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Autores principales: Šurín Hudáková, Natália, Kačírová, Jana, Sondorová, Miriam, Šelianová, Svetlana, Mucha, Rastislav, Maďar, Marián
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409769/
https://www.ncbi.nlm.nih.gov/pubmed/36013417
http://dx.doi.org/10.3390/life12081238
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author Šurín Hudáková, Natália
Kačírová, Jana
Sondorová, Miriam
Šelianová, Svetlana
Mucha, Rastislav
Maďar, Marián
author_facet Šurín Hudáková, Natália
Kačírová, Jana
Sondorová, Miriam
Šelianová, Svetlana
Mucha, Rastislav
Maďar, Marián
author_sort Šurín Hudáková, Natália
collection PubMed
description Bacillus licheniformis is used in a broad spectrum of areas, including some probiotic preparations for human and veterinary health. Moreover, B. licheniformis strains are known producers of various bioactive substances with antimicrobial and antibiofilm effects. In searching for new potentially beneficial bacteria for oral health, the inhibitory effect of B. licheniformis strains isolated from canine dental biofilm against pathogenic oral bacteria was evaluated. The antimicrobial effect of neutralized cell-free supernatants (nCFS) was assessed in vitro on polystyrene microtiter plates. Furthermore, molecular and morphological analyses were executed to evaluate the production of bioactive substances. To determine the nature of antimicrobial substance present in nCFS of B. licheniformis A-1-5B-AP, nCFS was exposed to the activity of various enzymes. The nCFS of B. licheniformis A-1-5B-AP significantly (p < 0.0001) reduced the growth of Porphyromonas gulae 3/H, Prevotella intermedia 1/P and Streptococcus mutans ATCC 35668. On the other hand, B. licheniformis A-2-11B-AP only significantly (p < 0.0001) inhibited the growth of P. intermedia 1/P and S. mutans ATCC 35668. However, enzyme-treated nCFS of B. licheniformis A-1-5B-AP did not lose its antimicrobial effect and significantly (p < 0.0001) inhibited the growth of Micrococcus luteus DSM 1790. Further studies are needed for the identification of antimicrobial substances.
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spelling pubmed-94097692022-08-26 Inhibitory Effect of Bacillus licheniformis Strains Isolated from Canine Oral Cavity Šurín Hudáková, Natália Kačírová, Jana Sondorová, Miriam Šelianová, Svetlana Mucha, Rastislav Maďar, Marián Life (Basel) Article Bacillus licheniformis is used in a broad spectrum of areas, including some probiotic preparations for human and veterinary health. Moreover, B. licheniformis strains are known producers of various bioactive substances with antimicrobial and antibiofilm effects. In searching for new potentially beneficial bacteria for oral health, the inhibitory effect of B. licheniformis strains isolated from canine dental biofilm against pathogenic oral bacteria was evaluated. The antimicrobial effect of neutralized cell-free supernatants (nCFS) was assessed in vitro on polystyrene microtiter plates. Furthermore, molecular and morphological analyses were executed to evaluate the production of bioactive substances. To determine the nature of antimicrobial substance present in nCFS of B. licheniformis A-1-5B-AP, nCFS was exposed to the activity of various enzymes. The nCFS of B. licheniformis A-1-5B-AP significantly (p < 0.0001) reduced the growth of Porphyromonas gulae 3/H, Prevotella intermedia 1/P and Streptococcus mutans ATCC 35668. On the other hand, B. licheniformis A-2-11B-AP only significantly (p < 0.0001) inhibited the growth of P. intermedia 1/P and S. mutans ATCC 35668. However, enzyme-treated nCFS of B. licheniformis A-1-5B-AP did not lose its antimicrobial effect and significantly (p < 0.0001) inhibited the growth of Micrococcus luteus DSM 1790. Further studies are needed for the identification of antimicrobial substances. MDPI 2022-08-15 /pmc/articles/PMC9409769/ /pubmed/36013417 http://dx.doi.org/10.3390/life12081238 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Šurín Hudáková, Natália
Kačírová, Jana
Sondorová, Miriam
Šelianová, Svetlana
Mucha, Rastislav
Maďar, Marián
Inhibitory Effect of Bacillus licheniformis Strains Isolated from Canine Oral Cavity
title Inhibitory Effect of Bacillus licheniformis Strains Isolated from Canine Oral Cavity
title_full Inhibitory Effect of Bacillus licheniformis Strains Isolated from Canine Oral Cavity
title_fullStr Inhibitory Effect of Bacillus licheniformis Strains Isolated from Canine Oral Cavity
title_full_unstemmed Inhibitory Effect of Bacillus licheniformis Strains Isolated from Canine Oral Cavity
title_short Inhibitory Effect of Bacillus licheniformis Strains Isolated from Canine Oral Cavity
title_sort inhibitory effect of bacillus licheniformis strains isolated from canine oral cavity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409769/
https://www.ncbi.nlm.nih.gov/pubmed/36013417
http://dx.doi.org/10.3390/life12081238
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