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Ultrasensitive and Selective Detection of Glutathione by Ammonium Carbamate–Gold Platinum Nanoparticles-Based Electrochemical Sensor

Determining the concentration of glutathione is crucial for developing workable medical diagnostic strategies. In this paper, we developed an electrochemical sensor by electrodepositing amino-based reactive groups and gold–platinum nanomaterials on the surface of glassy carbon electrode successively...

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Detalles Bibliográficos
Autores principales: Wang, Wei, Chen, Jiandan, Zhou, Zhenzeng, Zhan, Shanshan, Xing, Zhiyuan, Liu, Hongying, Zhang, Linan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9410014/
https://www.ncbi.nlm.nih.gov/pubmed/36013320
http://dx.doi.org/10.3390/life12081142
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author Wang, Wei
Chen, Jiandan
Zhou, Zhenzeng
Zhan, Shanshan
Xing, Zhiyuan
Liu, Hongying
Zhang, Linan
author_facet Wang, Wei
Chen, Jiandan
Zhou, Zhenzeng
Zhan, Shanshan
Xing, Zhiyuan
Liu, Hongying
Zhang, Linan
author_sort Wang, Wei
collection PubMed
description Determining the concentration of glutathione is crucial for developing workable medical diagnostic strategies. In this paper, we developed an electrochemical sensor by electrodepositing amino-based reactive groups and gold–platinum nanomaterials on the surface of glassy carbon electrode successively. The sensor was characterized by cyclic voltammetry (CV), field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDX), and electrochemical impedance spectra (EIS). Results showed that Au@Pt nanoparticles with the size of 20–40 nm were presented on the surface of electrode. The sensor exhibits excellent electrocatalytic oxidation towards glutathione. Based on this, we devised an electrochemical biosensor for rapid and sensitive detection of glutathione. After optimizing experimental and operational conditions, a linear response for the concentration of GSH, in the range of 0.1–11 μmol/L, with low detection and quantification limits of 0.051 μM (S/N = 3), were obtained. The sensor also exhibits superior selectivity, reproducibility, low cost, as well as simple preparation and can be applied in human serum sample detection.
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spelling pubmed-94100142022-08-26 Ultrasensitive and Selective Detection of Glutathione by Ammonium Carbamate–Gold Platinum Nanoparticles-Based Electrochemical Sensor Wang, Wei Chen, Jiandan Zhou, Zhenzeng Zhan, Shanshan Xing, Zhiyuan Liu, Hongying Zhang, Linan Life (Basel) Article Determining the concentration of glutathione is crucial for developing workable medical diagnostic strategies. In this paper, we developed an electrochemical sensor by electrodepositing amino-based reactive groups and gold–platinum nanomaterials on the surface of glassy carbon electrode successively. The sensor was characterized by cyclic voltammetry (CV), field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDX), and electrochemical impedance spectra (EIS). Results showed that Au@Pt nanoparticles with the size of 20–40 nm were presented on the surface of electrode. The sensor exhibits excellent electrocatalytic oxidation towards glutathione. Based on this, we devised an electrochemical biosensor for rapid and sensitive detection of glutathione. After optimizing experimental and operational conditions, a linear response for the concentration of GSH, in the range of 0.1–11 μmol/L, with low detection and quantification limits of 0.051 μM (S/N = 3), were obtained. The sensor also exhibits superior selectivity, reproducibility, low cost, as well as simple preparation and can be applied in human serum sample detection. MDPI 2022-07-28 /pmc/articles/PMC9410014/ /pubmed/36013320 http://dx.doi.org/10.3390/life12081142 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Wei
Chen, Jiandan
Zhou, Zhenzeng
Zhan, Shanshan
Xing, Zhiyuan
Liu, Hongying
Zhang, Linan
Ultrasensitive and Selective Detection of Glutathione by Ammonium Carbamate–Gold Platinum Nanoparticles-Based Electrochemical Sensor
title Ultrasensitive and Selective Detection of Glutathione by Ammonium Carbamate–Gold Platinum Nanoparticles-Based Electrochemical Sensor
title_full Ultrasensitive and Selective Detection of Glutathione by Ammonium Carbamate–Gold Platinum Nanoparticles-Based Electrochemical Sensor
title_fullStr Ultrasensitive and Selective Detection of Glutathione by Ammonium Carbamate–Gold Platinum Nanoparticles-Based Electrochemical Sensor
title_full_unstemmed Ultrasensitive and Selective Detection of Glutathione by Ammonium Carbamate–Gold Platinum Nanoparticles-Based Electrochemical Sensor
title_short Ultrasensitive and Selective Detection of Glutathione by Ammonium Carbamate–Gold Platinum Nanoparticles-Based Electrochemical Sensor
title_sort ultrasensitive and selective detection of glutathione by ammonium carbamate–gold platinum nanoparticles-based electrochemical sensor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9410014/
https://www.ncbi.nlm.nih.gov/pubmed/36013320
http://dx.doi.org/10.3390/life12081142
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