Cargando…
Ultrasensitive and Selective Detection of Glutathione by Ammonium Carbamate–Gold Platinum Nanoparticles-Based Electrochemical Sensor
Determining the concentration of glutathione is crucial for developing workable medical diagnostic strategies. In this paper, we developed an electrochemical sensor by electrodepositing amino-based reactive groups and gold–platinum nanomaterials on the surface of glassy carbon electrode successively...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9410014/ https://www.ncbi.nlm.nih.gov/pubmed/36013320 http://dx.doi.org/10.3390/life12081142 |
_version_ | 1784774991406956544 |
---|---|
author | Wang, Wei Chen, Jiandan Zhou, Zhenzeng Zhan, Shanshan Xing, Zhiyuan Liu, Hongying Zhang, Linan |
author_facet | Wang, Wei Chen, Jiandan Zhou, Zhenzeng Zhan, Shanshan Xing, Zhiyuan Liu, Hongying Zhang, Linan |
author_sort | Wang, Wei |
collection | PubMed |
description | Determining the concentration of glutathione is crucial for developing workable medical diagnostic strategies. In this paper, we developed an electrochemical sensor by electrodepositing amino-based reactive groups and gold–platinum nanomaterials on the surface of glassy carbon electrode successively. The sensor was characterized by cyclic voltammetry (CV), field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDX), and electrochemical impedance spectra (EIS). Results showed that Au@Pt nanoparticles with the size of 20–40 nm were presented on the surface of electrode. The sensor exhibits excellent electrocatalytic oxidation towards glutathione. Based on this, we devised an electrochemical biosensor for rapid and sensitive detection of glutathione. After optimizing experimental and operational conditions, a linear response for the concentration of GSH, in the range of 0.1–11 μmol/L, with low detection and quantification limits of 0.051 μM (S/N = 3), were obtained. The sensor also exhibits superior selectivity, reproducibility, low cost, as well as simple preparation and can be applied in human serum sample detection. |
format | Online Article Text |
id | pubmed-9410014 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94100142022-08-26 Ultrasensitive and Selective Detection of Glutathione by Ammonium Carbamate–Gold Platinum Nanoparticles-Based Electrochemical Sensor Wang, Wei Chen, Jiandan Zhou, Zhenzeng Zhan, Shanshan Xing, Zhiyuan Liu, Hongying Zhang, Linan Life (Basel) Article Determining the concentration of glutathione is crucial for developing workable medical diagnostic strategies. In this paper, we developed an electrochemical sensor by electrodepositing amino-based reactive groups and gold–platinum nanomaterials on the surface of glassy carbon electrode successively. The sensor was characterized by cyclic voltammetry (CV), field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDX), and electrochemical impedance spectra (EIS). Results showed that Au@Pt nanoparticles with the size of 20–40 nm were presented on the surface of electrode. The sensor exhibits excellent electrocatalytic oxidation towards glutathione. Based on this, we devised an electrochemical biosensor for rapid and sensitive detection of glutathione. After optimizing experimental and operational conditions, a linear response for the concentration of GSH, in the range of 0.1–11 μmol/L, with low detection and quantification limits of 0.051 μM (S/N = 3), were obtained. The sensor also exhibits superior selectivity, reproducibility, low cost, as well as simple preparation and can be applied in human serum sample detection. MDPI 2022-07-28 /pmc/articles/PMC9410014/ /pubmed/36013320 http://dx.doi.org/10.3390/life12081142 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Wei Chen, Jiandan Zhou, Zhenzeng Zhan, Shanshan Xing, Zhiyuan Liu, Hongying Zhang, Linan Ultrasensitive and Selective Detection of Glutathione by Ammonium Carbamate–Gold Platinum Nanoparticles-Based Electrochemical Sensor |
title | Ultrasensitive and Selective Detection of Glutathione by Ammonium Carbamate–Gold Platinum Nanoparticles-Based Electrochemical Sensor |
title_full | Ultrasensitive and Selective Detection of Glutathione by Ammonium Carbamate–Gold Platinum Nanoparticles-Based Electrochemical Sensor |
title_fullStr | Ultrasensitive and Selective Detection of Glutathione by Ammonium Carbamate–Gold Platinum Nanoparticles-Based Electrochemical Sensor |
title_full_unstemmed | Ultrasensitive and Selective Detection of Glutathione by Ammonium Carbamate–Gold Platinum Nanoparticles-Based Electrochemical Sensor |
title_short | Ultrasensitive and Selective Detection of Glutathione by Ammonium Carbamate–Gold Platinum Nanoparticles-Based Electrochemical Sensor |
title_sort | ultrasensitive and selective detection of glutathione by ammonium carbamate–gold platinum nanoparticles-based electrochemical sensor |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9410014/ https://www.ncbi.nlm.nih.gov/pubmed/36013320 http://dx.doi.org/10.3390/life12081142 |
work_keys_str_mv | AT wangwei ultrasensitiveandselectivedetectionofglutathionebyammoniumcarbamategoldplatinumnanoparticlesbasedelectrochemicalsensor AT chenjiandan ultrasensitiveandselectivedetectionofglutathionebyammoniumcarbamategoldplatinumnanoparticlesbasedelectrochemicalsensor AT zhouzhenzeng ultrasensitiveandselectivedetectionofglutathionebyammoniumcarbamategoldplatinumnanoparticlesbasedelectrochemicalsensor AT zhanshanshan ultrasensitiveandselectivedetectionofglutathionebyammoniumcarbamategoldplatinumnanoparticlesbasedelectrochemicalsensor AT xingzhiyuan ultrasensitiveandselectivedetectionofglutathionebyammoniumcarbamategoldplatinumnanoparticlesbasedelectrochemicalsensor AT liuhongying ultrasensitiveandselectivedetectionofglutathionebyammoniumcarbamategoldplatinumnanoparticlesbasedelectrochemicalsensor AT zhanglinan ultrasensitiveandselectivedetectionofglutathionebyammoniumcarbamategoldplatinumnanoparticlesbasedelectrochemicalsensor |