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Scale-Up to Pilot of a Non-Axenic Culture of Thraustochytrids Using Digestate from Methanization as Nitrogen Source

The production of non-fish based docosahexaenoic acid (DHA) for feed and food has become a critical need in our global context of over-fishing. The industrial-scale production of DHA–rich Thraustochytrids could be an alternative, if costs turned out to be competitive. In order to reduce production c...

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Autores principales: de la Broise, Denis, Ventura, Mariana, Chauchat, Luc, Guerreiro, Maurean, Michez, Teo, Vinet, Thibaud, Gautron, Nicolas, Le Grand, Fabienne, Bideau, Antoine, Goïc, Nelly Le, Bidault, Adeline, Lambert, Christophe, Soudant, Philippe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9410245/
https://www.ncbi.nlm.nih.gov/pubmed/36005502
http://dx.doi.org/10.3390/md20080499
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author de la Broise, Denis
Ventura, Mariana
Chauchat, Luc
Guerreiro, Maurean
Michez, Teo
Vinet, Thibaud
Gautron, Nicolas
Le Grand, Fabienne
Bideau, Antoine
Goïc, Nelly Le
Bidault, Adeline
Lambert, Christophe
Soudant, Philippe
author_facet de la Broise, Denis
Ventura, Mariana
Chauchat, Luc
Guerreiro, Maurean
Michez, Teo
Vinet, Thibaud
Gautron, Nicolas
Le Grand, Fabienne
Bideau, Antoine
Goïc, Nelly Le
Bidault, Adeline
Lambert, Christophe
Soudant, Philippe
author_sort de la Broise, Denis
collection PubMed
description The production of non-fish based docosahexaenoic acid (DHA) for feed and food has become a critical need in our global context of over-fishing. The industrial-scale production of DHA–rich Thraustochytrids could be an alternative, if costs turned out to be competitive. In order to reduce production costs, this study addresses the feasibility of the non-axenic (non-sterile) cultivation of Aurantiochytrium mangrovei on industrial substrates (as nitrogen and mineral sources and glucose syrup as carbon and energy sources), and its scale-up from laboratory (250 mL) to 500 L cultures. Pilot-scale reactors were airlift cylinders. Batch and fed-batch cultures were tested. Cultures over 38 to 62 h achieved a dry cell weight productivity of 3.3 to 5.5 g.L(−1).day(−1), and a substrate to biomass yield of up to 0.3. DHA productivity ranged from 10 to 0.18 mg.L(−1).day(−1). Biomass productivity appears linearly related to oxygen transfer rate. Bacterial contamination of cultures was low enough to avoid impacts on fatty acid composition of the biomass. A specific work on microbial risks assessment (in supplementary files) showed that the biomass can be securely used as feed. However, to date, there is a law void in EU legislation regarding the recycling of nitrogen from digestate from animal waste for microalgae biomass and its usage in animal feed. Overall, the proposed process appears similar to the industrial yeast production process (non-axenic heterotrophic process, dissolved oxygen supply limiting growth, similar cell size). Such similarity could help in further industrial developments.
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spelling pubmed-94102452022-08-26 Scale-Up to Pilot of a Non-Axenic Culture of Thraustochytrids Using Digestate from Methanization as Nitrogen Source de la Broise, Denis Ventura, Mariana Chauchat, Luc Guerreiro, Maurean Michez, Teo Vinet, Thibaud Gautron, Nicolas Le Grand, Fabienne Bideau, Antoine Goïc, Nelly Le Bidault, Adeline Lambert, Christophe Soudant, Philippe Mar Drugs Article The production of non-fish based docosahexaenoic acid (DHA) for feed and food has become a critical need in our global context of over-fishing. The industrial-scale production of DHA–rich Thraustochytrids could be an alternative, if costs turned out to be competitive. In order to reduce production costs, this study addresses the feasibility of the non-axenic (non-sterile) cultivation of Aurantiochytrium mangrovei on industrial substrates (as nitrogen and mineral sources and glucose syrup as carbon and energy sources), and its scale-up from laboratory (250 mL) to 500 L cultures. Pilot-scale reactors were airlift cylinders. Batch and fed-batch cultures were tested. Cultures over 38 to 62 h achieved a dry cell weight productivity of 3.3 to 5.5 g.L(−1).day(−1), and a substrate to biomass yield of up to 0.3. DHA productivity ranged from 10 to 0.18 mg.L(−1).day(−1). Biomass productivity appears linearly related to oxygen transfer rate. Bacterial contamination of cultures was low enough to avoid impacts on fatty acid composition of the biomass. A specific work on microbial risks assessment (in supplementary files) showed that the biomass can be securely used as feed. However, to date, there is a law void in EU legislation regarding the recycling of nitrogen from digestate from animal waste for microalgae biomass and its usage in animal feed. Overall, the proposed process appears similar to the industrial yeast production process (non-axenic heterotrophic process, dissolved oxygen supply limiting growth, similar cell size). Such similarity could help in further industrial developments. MDPI 2022-08-02 /pmc/articles/PMC9410245/ /pubmed/36005502 http://dx.doi.org/10.3390/md20080499 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
de la Broise, Denis
Ventura, Mariana
Chauchat, Luc
Guerreiro, Maurean
Michez, Teo
Vinet, Thibaud
Gautron, Nicolas
Le Grand, Fabienne
Bideau, Antoine
Goïc, Nelly Le
Bidault, Adeline
Lambert, Christophe
Soudant, Philippe
Scale-Up to Pilot of a Non-Axenic Culture of Thraustochytrids Using Digestate from Methanization as Nitrogen Source
title Scale-Up to Pilot of a Non-Axenic Culture of Thraustochytrids Using Digestate from Methanization as Nitrogen Source
title_full Scale-Up to Pilot of a Non-Axenic Culture of Thraustochytrids Using Digestate from Methanization as Nitrogen Source
title_fullStr Scale-Up to Pilot of a Non-Axenic Culture of Thraustochytrids Using Digestate from Methanization as Nitrogen Source
title_full_unstemmed Scale-Up to Pilot of a Non-Axenic Culture of Thraustochytrids Using Digestate from Methanization as Nitrogen Source
title_short Scale-Up to Pilot of a Non-Axenic Culture of Thraustochytrids Using Digestate from Methanization as Nitrogen Source
title_sort scale-up to pilot of a non-axenic culture of thraustochytrids using digestate from methanization as nitrogen source
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9410245/
https://www.ncbi.nlm.nih.gov/pubmed/36005502
http://dx.doi.org/10.3390/md20080499
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