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Differential Expression of Cell Wall Remodeling Genes Is Part of the Dynamic Phase-Specific Transcriptional Program of Conidial Germination of Trichoderma asperelloides

The nature of saprophytic and mycoparasitic hyphal growth of Trichoderma spp. has been studied extensively, yet its initiation via conidial germination in this genus is less well understood. Using near-synchronous germinating cultures of Trichoderma asperelloides, we followed the morphological progr...

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Autores principales: Gortikov, Maggie, Yakubovich, Elizabeta, Wang, Zheng, López-Giráldez, Francesc, Tu, Yujia, Townsend, Jeffrey P., Yarden, Oded
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9410309/
https://www.ncbi.nlm.nih.gov/pubmed/36012842
http://dx.doi.org/10.3390/jof8080854
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author Gortikov, Maggie
Yakubovich, Elizabeta
Wang, Zheng
López-Giráldez, Francesc
Tu, Yujia
Townsend, Jeffrey P.
Yarden, Oded
author_facet Gortikov, Maggie
Yakubovich, Elizabeta
Wang, Zheng
López-Giráldez, Francesc
Tu, Yujia
Townsend, Jeffrey P.
Yarden, Oded
author_sort Gortikov, Maggie
collection PubMed
description The nature of saprophytic and mycoparasitic hyphal growth of Trichoderma spp. has been studied extensively, yet its initiation via conidial germination in this genus is less well understood. Using near-synchronous germinating cultures of Trichoderma asperelloides, we followed the morphological progression from dormant conidia to initial polar growth to germling formation and to evidence for first branching. We found that the stage-specific transcriptional profile of T. asperelloides is one of the most dynamic described to date: transcript abundance of over 5000 genes—comprising approximately half of the annotated genome—was unremittingly reduced in the transition from dormancy to polar growth. Conversely, after the onset of germination, the transcript abundance of approximately a quarter of the genome was unremittingly elevated during the transition from elongation to initial branching. These changes are a testimony to the substantial developmental events that accompany germination. Bayesian network analysis identified several chitinase- and glucanase-encoding genes as active transcriptional hubs during germination. Furthermore, the expression of specific members of the chitin synthase and glucan elongase families was significantly increased during germination in the presence of Rhizoctonia solani—a known host of the mycoparasite—indicating that host recognition can occur during the early stages of mycoparasite development.
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spelling pubmed-94103092022-08-26 Differential Expression of Cell Wall Remodeling Genes Is Part of the Dynamic Phase-Specific Transcriptional Program of Conidial Germination of Trichoderma asperelloides Gortikov, Maggie Yakubovich, Elizabeta Wang, Zheng López-Giráldez, Francesc Tu, Yujia Townsend, Jeffrey P. Yarden, Oded J Fungi (Basel) Article The nature of saprophytic and mycoparasitic hyphal growth of Trichoderma spp. has been studied extensively, yet its initiation via conidial germination in this genus is less well understood. Using near-synchronous germinating cultures of Trichoderma asperelloides, we followed the morphological progression from dormant conidia to initial polar growth to germling formation and to evidence for first branching. We found that the stage-specific transcriptional profile of T. asperelloides is one of the most dynamic described to date: transcript abundance of over 5000 genes—comprising approximately half of the annotated genome—was unremittingly reduced in the transition from dormancy to polar growth. Conversely, after the onset of germination, the transcript abundance of approximately a quarter of the genome was unremittingly elevated during the transition from elongation to initial branching. These changes are a testimony to the substantial developmental events that accompany germination. Bayesian network analysis identified several chitinase- and glucanase-encoding genes as active transcriptional hubs during germination. Furthermore, the expression of specific members of the chitin synthase and glucan elongase families was significantly increased during germination in the presence of Rhizoctonia solani—a known host of the mycoparasite—indicating that host recognition can occur during the early stages of mycoparasite development. MDPI 2022-08-15 /pmc/articles/PMC9410309/ /pubmed/36012842 http://dx.doi.org/10.3390/jof8080854 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gortikov, Maggie
Yakubovich, Elizabeta
Wang, Zheng
López-Giráldez, Francesc
Tu, Yujia
Townsend, Jeffrey P.
Yarden, Oded
Differential Expression of Cell Wall Remodeling Genes Is Part of the Dynamic Phase-Specific Transcriptional Program of Conidial Germination of Trichoderma asperelloides
title Differential Expression of Cell Wall Remodeling Genes Is Part of the Dynamic Phase-Specific Transcriptional Program of Conidial Germination of Trichoderma asperelloides
title_full Differential Expression of Cell Wall Remodeling Genes Is Part of the Dynamic Phase-Specific Transcriptional Program of Conidial Germination of Trichoderma asperelloides
title_fullStr Differential Expression of Cell Wall Remodeling Genes Is Part of the Dynamic Phase-Specific Transcriptional Program of Conidial Germination of Trichoderma asperelloides
title_full_unstemmed Differential Expression of Cell Wall Remodeling Genes Is Part of the Dynamic Phase-Specific Transcriptional Program of Conidial Germination of Trichoderma asperelloides
title_short Differential Expression of Cell Wall Remodeling Genes Is Part of the Dynamic Phase-Specific Transcriptional Program of Conidial Germination of Trichoderma asperelloides
title_sort differential expression of cell wall remodeling genes is part of the dynamic phase-specific transcriptional program of conidial germination of trichoderma asperelloides
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9410309/
https://www.ncbi.nlm.nih.gov/pubmed/36012842
http://dx.doi.org/10.3390/jof8080854
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