Cargando…
The Elongator Subunit Elp3 Regulates Development, Stress Tolerance, Cell Cycle, and Virulence in the Entomopathogenic Fungus Beauveria bassiana
Transcriptional activity is mediated by chromatin remodeling, which in turn is affected by post-translational modifications, including histone acetylation. Histone acetyltransferases (HATs) are capable of promoting euchromatin formation and then activating gene transcription. Here, we characterize t...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9410351/ https://www.ncbi.nlm.nih.gov/pubmed/36012822 http://dx.doi.org/10.3390/jof8080834 |
_version_ | 1784775072419938304 |
---|---|
author | Cai, Qing Wang, Juanjuan Xie, Jiatao Jiang, Daohong Keyhani, Nemat O. |
author_facet | Cai, Qing Wang, Juanjuan Xie, Jiatao Jiang, Daohong Keyhani, Nemat O. |
author_sort | Cai, Qing |
collection | PubMed |
description | Transcriptional activity is mediated by chromatin remodeling, which in turn is affected by post-translational modifications, including histone acetylation. Histone acetyltransferases (HATs) are capable of promoting euchromatin formation and then activating gene transcription. Here, we characterize the Elp3 GNAT family HAT, which is also a subunit of Elongator complex, in the environmentally and economically important fungal insect pathogen, Beauveria bassiana. BbElp3 showed high localization levels to mitochondria, with some nuclear and cytoplasmic localization also apparent. Targeted gene knockout of BbElp3 resulted in impaired asexual development and morphogenesis, reduced tolerances to multiple stress conditions, reduced the ability of the fungus to utilize various carbon/nitrogen sources, increased susceptibility to rapamycin, and attenuated virulence in bioassays using the greater wax moth, Galleria mellonella. The ΔBbElp3 mutant also showed disrupted cell cycle, abnormal hyphal septation patterns, and enlarged autophagosomes in vegetative hyphae. Transcriptome analyses revealed differential expression of 775 genes (DEGs), including 336 downregulated and 438 upregulated genes in the ΔBbElp3 strain as compared to the wild type. Downregulated genes were mainly enriched in pathways involved in DNA processing and transcription, cell cycle control, cellular transportation, cell defense, and virulence, including hydrophobins, cellular transporters (ABC and MFS multidrug transporters), and insect cuticular degrading enzymes, while upregulated genes were mainly enriched in carbohydrate metabolism and amino acid metabolism. These data indicate pleiotropic effects of BbElp3 in impacting specific cellular processes related to asexual development, cell cycle, autophagy, and virulence. |
format | Online Article Text |
id | pubmed-9410351 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94103512022-08-26 The Elongator Subunit Elp3 Regulates Development, Stress Tolerance, Cell Cycle, and Virulence in the Entomopathogenic Fungus Beauveria bassiana Cai, Qing Wang, Juanjuan Xie, Jiatao Jiang, Daohong Keyhani, Nemat O. J Fungi (Basel) Article Transcriptional activity is mediated by chromatin remodeling, which in turn is affected by post-translational modifications, including histone acetylation. Histone acetyltransferases (HATs) are capable of promoting euchromatin formation and then activating gene transcription. Here, we characterize the Elp3 GNAT family HAT, which is also a subunit of Elongator complex, in the environmentally and economically important fungal insect pathogen, Beauveria bassiana. BbElp3 showed high localization levels to mitochondria, with some nuclear and cytoplasmic localization also apparent. Targeted gene knockout of BbElp3 resulted in impaired asexual development and morphogenesis, reduced tolerances to multiple stress conditions, reduced the ability of the fungus to utilize various carbon/nitrogen sources, increased susceptibility to rapamycin, and attenuated virulence in bioassays using the greater wax moth, Galleria mellonella. The ΔBbElp3 mutant also showed disrupted cell cycle, abnormal hyphal septation patterns, and enlarged autophagosomes in vegetative hyphae. Transcriptome analyses revealed differential expression of 775 genes (DEGs), including 336 downregulated and 438 upregulated genes in the ΔBbElp3 strain as compared to the wild type. Downregulated genes were mainly enriched in pathways involved in DNA processing and transcription, cell cycle control, cellular transportation, cell defense, and virulence, including hydrophobins, cellular transporters (ABC and MFS multidrug transporters), and insect cuticular degrading enzymes, while upregulated genes were mainly enriched in carbohydrate metabolism and amino acid metabolism. These data indicate pleiotropic effects of BbElp3 in impacting specific cellular processes related to asexual development, cell cycle, autophagy, and virulence. MDPI 2022-08-10 /pmc/articles/PMC9410351/ /pubmed/36012822 http://dx.doi.org/10.3390/jof8080834 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cai, Qing Wang, Juanjuan Xie, Jiatao Jiang, Daohong Keyhani, Nemat O. The Elongator Subunit Elp3 Regulates Development, Stress Tolerance, Cell Cycle, and Virulence in the Entomopathogenic Fungus Beauveria bassiana |
title | The Elongator Subunit Elp3 Regulates Development, Stress Tolerance, Cell Cycle, and Virulence in the Entomopathogenic Fungus Beauveria bassiana |
title_full | The Elongator Subunit Elp3 Regulates Development, Stress Tolerance, Cell Cycle, and Virulence in the Entomopathogenic Fungus Beauveria bassiana |
title_fullStr | The Elongator Subunit Elp3 Regulates Development, Stress Tolerance, Cell Cycle, and Virulence in the Entomopathogenic Fungus Beauveria bassiana |
title_full_unstemmed | The Elongator Subunit Elp3 Regulates Development, Stress Tolerance, Cell Cycle, and Virulence in the Entomopathogenic Fungus Beauveria bassiana |
title_short | The Elongator Subunit Elp3 Regulates Development, Stress Tolerance, Cell Cycle, and Virulence in the Entomopathogenic Fungus Beauveria bassiana |
title_sort | elongator subunit elp3 regulates development, stress tolerance, cell cycle, and virulence in the entomopathogenic fungus beauveria bassiana |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9410351/ https://www.ncbi.nlm.nih.gov/pubmed/36012822 http://dx.doi.org/10.3390/jof8080834 |
work_keys_str_mv | AT caiqing theelongatorsubunitelp3regulatesdevelopmentstresstolerancecellcycleandvirulenceintheentomopathogenicfungusbeauveriabassiana AT wangjuanjuan theelongatorsubunitelp3regulatesdevelopmentstresstolerancecellcycleandvirulenceintheentomopathogenicfungusbeauveriabassiana AT xiejiatao theelongatorsubunitelp3regulatesdevelopmentstresstolerancecellcycleandvirulenceintheentomopathogenicfungusbeauveriabassiana AT jiangdaohong theelongatorsubunitelp3regulatesdevelopmentstresstolerancecellcycleandvirulenceintheentomopathogenicfungusbeauveriabassiana AT keyhaninemato theelongatorsubunitelp3regulatesdevelopmentstresstolerancecellcycleandvirulenceintheentomopathogenicfungusbeauveriabassiana AT caiqing elongatorsubunitelp3regulatesdevelopmentstresstolerancecellcycleandvirulenceintheentomopathogenicfungusbeauveriabassiana AT wangjuanjuan elongatorsubunitelp3regulatesdevelopmentstresstolerancecellcycleandvirulenceintheentomopathogenicfungusbeauveriabassiana AT xiejiatao elongatorsubunitelp3regulatesdevelopmentstresstolerancecellcycleandvirulenceintheentomopathogenicfungusbeauveriabassiana AT jiangdaohong elongatorsubunitelp3regulatesdevelopmentstresstolerancecellcycleandvirulenceintheentomopathogenicfungusbeauveriabassiana AT keyhaninemato elongatorsubunitelp3regulatesdevelopmentstresstolerancecellcycleandvirulenceintheentomopathogenicfungusbeauveriabassiana |