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World Health Organization critical priority Escherichia coli clone ST648 in magnificent frigatebird (Fregata magnificens) of an uninhabited insular environment

Antimicrobial resistance is an ancient natural phenomenon increasingly pressured by anthropogenic activities. Escherichia coli has been used as markers of environmental contamination and human-related activity. Seabirds may be bioindicators of clinically relevant bacterial pathogens and their antimi...

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Autores principales: Ewbank, Ana Carolina, Fuentes-Castillo, Danny, Sacristán, Carlos, Esposito, Fernanda, Fuga, Bruna, Cardoso, Brenda, Godoy, Silvia Neri, Zamana, Roberta Ramblas, Gattamorta, Marco Aurélio, Catão-Dias, José Luiz, Lincopan, Nilton
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9410367/
https://www.ncbi.nlm.nih.gov/pubmed/36033868
http://dx.doi.org/10.3389/fmicb.2022.940600
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author Ewbank, Ana Carolina
Fuentes-Castillo, Danny
Sacristán, Carlos
Esposito, Fernanda
Fuga, Bruna
Cardoso, Brenda
Godoy, Silvia Neri
Zamana, Roberta Ramblas
Gattamorta, Marco Aurélio
Catão-Dias, José Luiz
Lincopan, Nilton
author_facet Ewbank, Ana Carolina
Fuentes-Castillo, Danny
Sacristán, Carlos
Esposito, Fernanda
Fuga, Bruna
Cardoso, Brenda
Godoy, Silvia Neri
Zamana, Roberta Ramblas
Gattamorta, Marco Aurélio
Catão-Dias, José Luiz
Lincopan, Nilton
author_sort Ewbank, Ana Carolina
collection PubMed
description Antimicrobial resistance is an ancient natural phenomenon increasingly pressured by anthropogenic activities. Escherichia coli has been used as markers of environmental contamination and human-related activity. Seabirds may be bioindicators of clinically relevant bacterial pathogens and their antimicrobial resistance genes, including extended-spectrum-beta-lactamase (ESBL) and/or plasmid-encoded AmpC (pAmpC), in anthropized and remote areas. We evaluated cloacal swabs of 20 wild magnificent frigatebirds (Fregata magnificens) of the Alcatrazes Archipelago, the biggest breeding colony of magnificent frigatebirds in the southern Atlantic and a natural protected area with no history of human occupation, located in the anthropized southeastern Brazilian coast. We characterized a highly virulent multidrug-resistant ST648 (O153:H9) pandemic clone, harboring bla(CTX–M–2), bla(CMY–2), qnrB, tetB, sul1, sul2, aadA1, aac(3)-VIa and mdfA, and virulence genes characteristic of avian pathogenic (APEC) (hlyF, iroN, iss, iutA, and ompT) and other extraintestinal E. coli (ExPEC) (chuA, kpsMII, and papC). To our knowledge, this is the first report of ST648 E. coli co-producing ESBL and pAmpC in wild birds inhabiting insular environments. We suggest this potentially zoonotic and pathogenic lineage was likely acquired through indirect anthropogenic contamination of the marine environment, ingestion of contaminated seafood, or by intra and/or interspecific contact. Our findings reinforce the role of wild birds as anthropization sentinels in insular environments and the importance of wildlife surveillance studies on pathogens of critical priority classified by the World Health Organization.
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spelling pubmed-94103672022-08-26 World Health Organization critical priority Escherichia coli clone ST648 in magnificent frigatebird (Fregata magnificens) of an uninhabited insular environment Ewbank, Ana Carolina Fuentes-Castillo, Danny Sacristán, Carlos Esposito, Fernanda Fuga, Bruna Cardoso, Brenda Godoy, Silvia Neri Zamana, Roberta Ramblas Gattamorta, Marco Aurélio Catão-Dias, José Luiz Lincopan, Nilton Front Microbiol Microbiology Antimicrobial resistance is an ancient natural phenomenon increasingly pressured by anthropogenic activities. Escherichia coli has been used as markers of environmental contamination and human-related activity. Seabirds may be bioindicators of clinically relevant bacterial pathogens and their antimicrobial resistance genes, including extended-spectrum-beta-lactamase (ESBL) and/or plasmid-encoded AmpC (pAmpC), in anthropized and remote areas. We evaluated cloacal swabs of 20 wild magnificent frigatebirds (Fregata magnificens) of the Alcatrazes Archipelago, the biggest breeding colony of magnificent frigatebirds in the southern Atlantic and a natural protected area with no history of human occupation, located in the anthropized southeastern Brazilian coast. We characterized a highly virulent multidrug-resistant ST648 (O153:H9) pandemic clone, harboring bla(CTX–M–2), bla(CMY–2), qnrB, tetB, sul1, sul2, aadA1, aac(3)-VIa and mdfA, and virulence genes characteristic of avian pathogenic (APEC) (hlyF, iroN, iss, iutA, and ompT) and other extraintestinal E. coli (ExPEC) (chuA, kpsMII, and papC). To our knowledge, this is the first report of ST648 E. coli co-producing ESBL and pAmpC in wild birds inhabiting insular environments. We suggest this potentially zoonotic and pathogenic lineage was likely acquired through indirect anthropogenic contamination of the marine environment, ingestion of contaminated seafood, or by intra and/or interspecific contact. Our findings reinforce the role of wild birds as anthropization sentinels in insular environments and the importance of wildlife surveillance studies on pathogens of critical priority classified by the World Health Organization. Frontiers Media S.A. 2022-08-11 /pmc/articles/PMC9410367/ /pubmed/36033868 http://dx.doi.org/10.3389/fmicb.2022.940600 Text en Copyright © 2022 Ewbank, Fuentes-Castillo, Sacristán, Esposito, Fuga, Cardoso, Godoy, Zamana, Gattamorta, Catão-Dias and Lincopan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Ewbank, Ana Carolina
Fuentes-Castillo, Danny
Sacristán, Carlos
Esposito, Fernanda
Fuga, Bruna
Cardoso, Brenda
Godoy, Silvia Neri
Zamana, Roberta Ramblas
Gattamorta, Marco Aurélio
Catão-Dias, José Luiz
Lincopan, Nilton
World Health Organization critical priority Escherichia coli clone ST648 in magnificent frigatebird (Fregata magnificens) of an uninhabited insular environment
title World Health Organization critical priority Escherichia coli clone ST648 in magnificent frigatebird (Fregata magnificens) of an uninhabited insular environment
title_full World Health Organization critical priority Escherichia coli clone ST648 in magnificent frigatebird (Fregata magnificens) of an uninhabited insular environment
title_fullStr World Health Organization critical priority Escherichia coli clone ST648 in magnificent frigatebird (Fregata magnificens) of an uninhabited insular environment
title_full_unstemmed World Health Organization critical priority Escherichia coli clone ST648 in magnificent frigatebird (Fregata magnificens) of an uninhabited insular environment
title_short World Health Organization critical priority Escherichia coli clone ST648 in magnificent frigatebird (Fregata magnificens) of an uninhabited insular environment
title_sort world health organization critical priority escherichia coli clone st648 in magnificent frigatebird (fregata magnificens) of an uninhabited insular environment
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9410367/
https://www.ncbi.nlm.nih.gov/pubmed/36033868
http://dx.doi.org/10.3389/fmicb.2022.940600
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