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Antioxidant Activity of Fucoidan Modified with Gallic Acid Using the Redox Method
Antioxidant compounds decrease the amount of intracellular reactive oxygen species (ROS) and, consequently, reduce the deleterious effects of ROS in osteoblasts. Here, we modified a 21 kDa fucoidan (FucA) with gallic acid (GA) using the redox method, to potentiate its antioxidant/protective capacity...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9410456/ https://www.ncbi.nlm.nih.gov/pubmed/36005493 http://dx.doi.org/10.3390/md20080490 |
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author | de Melo, Keylla Dayanne Coelho Marinho Lisboa, Lucas dos Santos Queiroz, Moacir Fernandes Paiva, Weslley Souza Luchiari, Ana Carolina Camara, Rafael Barros Gomes Costa, Leandro Silva Rocha, Hugo Alexandre Oliveira |
author_facet | de Melo, Keylla Dayanne Coelho Marinho Lisboa, Lucas dos Santos Queiroz, Moacir Fernandes Paiva, Weslley Souza Luchiari, Ana Carolina Camara, Rafael Barros Gomes Costa, Leandro Silva Rocha, Hugo Alexandre Oliveira |
author_sort | de Melo, Keylla Dayanne Coelho Marinho |
collection | PubMed |
description | Antioxidant compounds decrease the amount of intracellular reactive oxygen species (ROS) and, consequently, reduce the deleterious effects of ROS in osteoblasts. Here, we modified a 21 kDa fucoidan (FucA) with gallic acid (GA) using the redox method, to potentiate its antioxidant/protective capacity on pre-osteoblast-like cells (MC3T3) against oxidative stress. The 20 kDa FucA-GA contains 37 ± 3.0 mg GA per gram of FucA. FucA-GA was the most efficient antioxidant agent in terms of total antioxidant capacity (2.5 times), reducing power (five times), copper chelation (three times), and superoxide radical scavenging (2 times). Exposure of MC3T3 cells to H(2)O(2) increased ROS levels and activated caspase-3 along with caspase-9. In addition, the cell viability decreased approximately 80%. FucA-GA also provided the most effective protection against oxidative damage caused by H(2)O(2). Treatment with FucA-GA (1.0 mg/mL) increased cell viability (~80%) and decreased intracellular ROS (100%) and caspase activation (~80%). In addition, Fuc-GA (0.1 mg/mL) abolished H(2)O(2)-induced oxidative stress in zebra fish embryos. Overall, FucA-GA protected MC3T3 cells from oxidative stress and could represent a possible adjuvant for the treatment of bone fragility by counteracting oxidative phenomena. |
format | Online Article Text |
id | pubmed-9410456 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94104562022-08-26 Antioxidant Activity of Fucoidan Modified with Gallic Acid Using the Redox Method de Melo, Keylla Dayanne Coelho Marinho Lisboa, Lucas dos Santos Queiroz, Moacir Fernandes Paiva, Weslley Souza Luchiari, Ana Carolina Camara, Rafael Barros Gomes Costa, Leandro Silva Rocha, Hugo Alexandre Oliveira Mar Drugs Article Antioxidant compounds decrease the amount of intracellular reactive oxygen species (ROS) and, consequently, reduce the deleterious effects of ROS in osteoblasts. Here, we modified a 21 kDa fucoidan (FucA) with gallic acid (GA) using the redox method, to potentiate its antioxidant/protective capacity on pre-osteoblast-like cells (MC3T3) against oxidative stress. The 20 kDa FucA-GA contains 37 ± 3.0 mg GA per gram of FucA. FucA-GA was the most efficient antioxidant agent in terms of total antioxidant capacity (2.5 times), reducing power (five times), copper chelation (three times), and superoxide radical scavenging (2 times). Exposure of MC3T3 cells to H(2)O(2) increased ROS levels and activated caspase-3 along with caspase-9. In addition, the cell viability decreased approximately 80%. FucA-GA also provided the most effective protection against oxidative damage caused by H(2)O(2). Treatment with FucA-GA (1.0 mg/mL) increased cell viability (~80%) and decreased intracellular ROS (100%) and caspase activation (~80%). In addition, Fuc-GA (0.1 mg/mL) abolished H(2)O(2)-induced oxidative stress in zebra fish embryos. Overall, FucA-GA protected MC3T3 cells from oxidative stress and could represent a possible adjuvant for the treatment of bone fragility by counteracting oxidative phenomena. MDPI 2022-07-29 /pmc/articles/PMC9410456/ /pubmed/36005493 http://dx.doi.org/10.3390/md20080490 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article de Melo, Keylla Dayanne Coelho Marinho Lisboa, Lucas dos Santos Queiroz, Moacir Fernandes Paiva, Weslley Souza Luchiari, Ana Carolina Camara, Rafael Barros Gomes Costa, Leandro Silva Rocha, Hugo Alexandre Oliveira Antioxidant Activity of Fucoidan Modified with Gallic Acid Using the Redox Method |
title | Antioxidant Activity of Fucoidan Modified with Gallic Acid Using the Redox Method |
title_full | Antioxidant Activity of Fucoidan Modified with Gallic Acid Using the Redox Method |
title_fullStr | Antioxidant Activity of Fucoidan Modified with Gallic Acid Using the Redox Method |
title_full_unstemmed | Antioxidant Activity of Fucoidan Modified with Gallic Acid Using the Redox Method |
title_short | Antioxidant Activity of Fucoidan Modified with Gallic Acid Using the Redox Method |
title_sort | antioxidant activity of fucoidan modified with gallic acid using the redox method |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9410456/ https://www.ncbi.nlm.nih.gov/pubmed/36005493 http://dx.doi.org/10.3390/md20080490 |
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