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Antioxidant Activity of Fucoidan Modified with Gallic Acid Using the Redox Method

Antioxidant compounds decrease the amount of intracellular reactive oxygen species (ROS) and, consequently, reduce the deleterious effects of ROS in osteoblasts. Here, we modified a 21 kDa fucoidan (FucA) with gallic acid (GA) using the redox method, to potentiate its antioxidant/protective capacity...

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Autores principales: de Melo, Keylla Dayanne Coelho Marinho, Lisboa, Lucas dos Santos, Queiroz, Moacir Fernandes, Paiva, Weslley Souza, Luchiari, Ana Carolina, Camara, Rafael Barros Gomes, Costa, Leandro Silva, Rocha, Hugo Alexandre Oliveira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9410456/
https://www.ncbi.nlm.nih.gov/pubmed/36005493
http://dx.doi.org/10.3390/md20080490
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author de Melo, Keylla Dayanne Coelho Marinho
Lisboa, Lucas dos Santos
Queiroz, Moacir Fernandes
Paiva, Weslley Souza
Luchiari, Ana Carolina
Camara, Rafael Barros Gomes
Costa, Leandro Silva
Rocha, Hugo Alexandre Oliveira
author_facet de Melo, Keylla Dayanne Coelho Marinho
Lisboa, Lucas dos Santos
Queiroz, Moacir Fernandes
Paiva, Weslley Souza
Luchiari, Ana Carolina
Camara, Rafael Barros Gomes
Costa, Leandro Silva
Rocha, Hugo Alexandre Oliveira
author_sort de Melo, Keylla Dayanne Coelho Marinho
collection PubMed
description Antioxidant compounds decrease the amount of intracellular reactive oxygen species (ROS) and, consequently, reduce the deleterious effects of ROS in osteoblasts. Here, we modified a 21 kDa fucoidan (FucA) with gallic acid (GA) using the redox method, to potentiate its antioxidant/protective capacity on pre-osteoblast-like cells (MC3T3) against oxidative stress. The 20 kDa FucA-GA contains 37 ± 3.0 mg GA per gram of FucA. FucA-GA was the most efficient antioxidant agent in terms of total antioxidant capacity (2.5 times), reducing power (five times), copper chelation (three times), and superoxide radical scavenging (2 times). Exposure of MC3T3 cells to H(2)O(2) increased ROS levels and activated caspase-3 along with caspase-9. In addition, the cell viability decreased approximately 80%. FucA-GA also provided the most effective protection against oxidative damage caused by H(2)O(2). Treatment with FucA-GA (1.0 mg/mL) increased cell viability (~80%) and decreased intracellular ROS (100%) and caspase activation (~80%). In addition, Fuc-GA (0.1 mg/mL) abolished H(2)O(2)-induced oxidative stress in zebra fish embryos. Overall, FucA-GA protected MC3T3 cells from oxidative stress and could represent a possible adjuvant for the treatment of bone fragility by counteracting oxidative phenomena.
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spelling pubmed-94104562022-08-26 Antioxidant Activity of Fucoidan Modified with Gallic Acid Using the Redox Method de Melo, Keylla Dayanne Coelho Marinho Lisboa, Lucas dos Santos Queiroz, Moacir Fernandes Paiva, Weslley Souza Luchiari, Ana Carolina Camara, Rafael Barros Gomes Costa, Leandro Silva Rocha, Hugo Alexandre Oliveira Mar Drugs Article Antioxidant compounds decrease the amount of intracellular reactive oxygen species (ROS) and, consequently, reduce the deleterious effects of ROS in osteoblasts. Here, we modified a 21 kDa fucoidan (FucA) with gallic acid (GA) using the redox method, to potentiate its antioxidant/protective capacity on pre-osteoblast-like cells (MC3T3) against oxidative stress. The 20 kDa FucA-GA contains 37 ± 3.0 mg GA per gram of FucA. FucA-GA was the most efficient antioxidant agent in terms of total antioxidant capacity (2.5 times), reducing power (five times), copper chelation (three times), and superoxide radical scavenging (2 times). Exposure of MC3T3 cells to H(2)O(2) increased ROS levels and activated caspase-3 along with caspase-9. In addition, the cell viability decreased approximately 80%. FucA-GA also provided the most effective protection against oxidative damage caused by H(2)O(2). Treatment with FucA-GA (1.0 mg/mL) increased cell viability (~80%) and decreased intracellular ROS (100%) and caspase activation (~80%). In addition, Fuc-GA (0.1 mg/mL) abolished H(2)O(2)-induced oxidative stress in zebra fish embryos. Overall, FucA-GA protected MC3T3 cells from oxidative stress and could represent a possible adjuvant for the treatment of bone fragility by counteracting oxidative phenomena. MDPI 2022-07-29 /pmc/articles/PMC9410456/ /pubmed/36005493 http://dx.doi.org/10.3390/md20080490 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
de Melo, Keylla Dayanne Coelho Marinho
Lisboa, Lucas dos Santos
Queiroz, Moacir Fernandes
Paiva, Weslley Souza
Luchiari, Ana Carolina
Camara, Rafael Barros Gomes
Costa, Leandro Silva
Rocha, Hugo Alexandre Oliveira
Antioxidant Activity of Fucoidan Modified with Gallic Acid Using the Redox Method
title Antioxidant Activity of Fucoidan Modified with Gallic Acid Using the Redox Method
title_full Antioxidant Activity of Fucoidan Modified with Gallic Acid Using the Redox Method
title_fullStr Antioxidant Activity of Fucoidan Modified with Gallic Acid Using the Redox Method
title_full_unstemmed Antioxidant Activity of Fucoidan Modified with Gallic Acid Using the Redox Method
title_short Antioxidant Activity of Fucoidan Modified with Gallic Acid Using the Redox Method
title_sort antioxidant activity of fucoidan modified with gallic acid using the redox method
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9410456/
https://www.ncbi.nlm.nih.gov/pubmed/36005493
http://dx.doi.org/10.3390/md20080490
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