Cargando…
The Sorting Nexin Genes ChSNX4 and ChSNX41 Are Required for Reproductive Development, Stress Adaption and Virulence in Cochliobolus heterostrophus
Sorting nexins are a conserved protein family involved in many cellular processes in fungi, and the function of sorting nexin Snx4 (Atg24) and Snx41 (Atg20) in Cochliobolus heterostrophus was not clear. The ΔChsnx4 and ΔChsnx41 mutants were generated by a PCR-based marker method to determine the rol...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9410474/ https://www.ncbi.nlm.nih.gov/pubmed/36012843 http://dx.doi.org/10.3390/jof8080855 |
_version_ | 1784775102138679296 |
---|---|
author | Yu, Huilin Jia, Wantong Li, Zhongxiang Gao, Chaofeng Pan, Hongyu Zhang, Xianghui |
author_facet | Yu, Huilin Jia, Wantong Li, Zhongxiang Gao, Chaofeng Pan, Hongyu Zhang, Xianghui |
author_sort | Yu, Huilin |
collection | PubMed |
description | Sorting nexins are a conserved protein family involved in many cellular processes in fungi, and the function of sorting nexin Snx4 (Atg24) and Snx41 (Atg20) in Cochliobolus heterostrophus was not clear. The ΔChsnx4 and ΔChsnx41 mutants were generated by a PCR-based marker method to determine the roles of Snx4 and Snx41 in reproductive development, stress adaption, and virulence in C. heterostrophus. Compared with the wild-type strain, the ΔChsnx4 and ΔChsnx41 mutants exhibited obvious changes in vegetative growth and in morphology of conidia. In addition, the conidiation, appressorium formation, and virulence of snx4 and snx41 mutants were dramatically reduced. Moreover, ΔChsnx4 and ΔChsnx41 mutants were more sensitive to oxidative stress (menadione and H(2)O(2)), cell wall integrity stress (Congo red and calcofluor white), fungicides, and isothiocyanates. All the phenotypes mentioned above were restored in complemented strains. In addition, ChSnx4 and ChSnx41 were proven to interact with each other through yeast two-hybrid. Taken together, these findings indicated that ChSNX4 and ChSNX41 were important for fungal growth, asexual development, stress adaption, and virulence in C. heterostrophus. |
format | Online Article Text |
id | pubmed-9410474 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94104742022-08-26 The Sorting Nexin Genes ChSNX4 and ChSNX41 Are Required for Reproductive Development, Stress Adaption and Virulence in Cochliobolus heterostrophus Yu, Huilin Jia, Wantong Li, Zhongxiang Gao, Chaofeng Pan, Hongyu Zhang, Xianghui J Fungi (Basel) Article Sorting nexins are a conserved protein family involved in many cellular processes in fungi, and the function of sorting nexin Snx4 (Atg24) and Snx41 (Atg20) in Cochliobolus heterostrophus was not clear. The ΔChsnx4 and ΔChsnx41 mutants were generated by a PCR-based marker method to determine the roles of Snx4 and Snx41 in reproductive development, stress adaption, and virulence in C. heterostrophus. Compared with the wild-type strain, the ΔChsnx4 and ΔChsnx41 mutants exhibited obvious changes in vegetative growth and in morphology of conidia. In addition, the conidiation, appressorium formation, and virulence of snx4 and snx41 mutants were dramatically reduced. Moreover, ΔChsnx4 and ΔChsnx41 mutants were more sensitive to oxidative stress (menadione and H(2)O(2)), cell wall integrity stress (Congo red and calcofluor white), fungicides, and isothiocyanates. All the phenotypes mentioned above were restored in complemented strains. In addition, ChSnx4 and ChSnx41 were proven to interact with each other through yeast two-hybrid. Taken together, these findings indicated that ChSNX4 and ChSNX41 were important for fungal growth, asexual development, stress adaption, and virulence in C. heterostrophus. MDPI 2022-08-15 /pmc/articles/PMC9410474/ /pubmed/36012843 http://dx.doi.org/10.3390/jof8080855 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yu, Huilin Jia, Wantong Li, Zhongxiang Gao, Chaofeng Pan, Hongyu Zhang, Xianghui The Sorting Nexin Genes ChSNX4 and ChSNX41 Are Required for Reproductive Development, Stress Adaption and Virulence in Cochliobolus heterostrophus |
title | The Sorting Nexin Genes ChSNX4 and ChSNX41 Are Required for Reproductive Development, Stress Adaption and Virulence in Cochliobolus heterostrophus |
title_full | The Sorting Nexin Genes ChSNX4 and ChSNX41 Are Required for Reproductive Development, Stress Adaption and Virulence in Cochliobolus heterostrophus |
title_fullStr | The Sorting Nexin Genes ChSNX4 and ChSNX41 Are Required for Reproductive Development, Stress Adaption and Virulence in Cochliobolus heterostrophus |
title_full_unstemmed | The Sorting Nexin Genes ChSNX4 and ChSNX41 Are Required for Reproductive Development, Stress Adaption and Virulence in Cochliobolus heterostrophus |
title_short | The Sorting Nexin Genes ChSNX4 and ChSNX41 Are Required for Reproductive Development, Stress Adaption and Virulence in Cochliobolus heterostrophus |
title_sort | sorting nexin genes chsnx4 and chsnx41 are required for reproductive development, stress adaption and virulence in cochliobolus heterostrophus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9410474/ https://www.ncbi.nlm.nih.gov/pubmed/36012843 http://dx.doi.org/10.3390/jof8080855 |
work_keys_str_mv | AT yuhuilin thesortingnexingeneschsnx4andchsnx41arerequiredforreproductivedevelopmentstressadaptionandvirulenceincochliobolusheterostrophus AT jiawantong thesortingnexingeneschsnx4andchsnx41arerequiredforreproductivedevelopmentstressadaptionandvirulenceincochliobolusheterostrophus AT lizhongxiang thesortingnexingeneschsnx4andchsnx41arerequiredforreproductivedevelopmentstressadaptionandvirulenceincochliobolusheterostrophus AT gaochaofeng thesortingnexingeneschsnx4andchsnx41arerequiredforreproductivedevelopmentstressadaptionandvirulenceincochliobolusheterostrophus AT panhongyu thesortingnexingeneschsnx4andchsnx41arerequiredforreproductivedevelopmentstressadaptionandvirulenceincochliobolusheterostrophus AT zhangxianghui thesortingnexingeneschsnx4andchsnx41arerequiredforreproductivedevelopmentstressadaptionandvirulenceincochliobolusheterostrophus AT yuhuilin sortingnexingeneschsnx4andchsnx41arerequiredforreproductivedevelopmentstressadaptionandvirulenceincochliobolusheterostrophus AT jiawantong sortingnexingeneschsnx4andchsnx41arerequiredforreproductivedevelopmentstressadaptionandvirulenceincochliobolusheterostrophus AT lizhongxiang sortingnexingeneschsnx4andchsnx41arerequiredforreproductivedevelopmentstressadaptionandvirulenceincochliobolusheterostrophus AT gaochaofeng sortingnexingeneschsnx4andchsnx41arerequiredforreproductivedevelopmentstressadaptionandvirulenceincochliobolusheterostrophus AT panhongyu sortingnexingeneschsnx4andchsnx41arerequiredforreproductivedevelopmentstressadaptionandvirulenceincochliobolusheterostrophus AT zhangxianghui sortingnexingeneschsnx4andchsnx41arerequiredforreproductivedevelopmentstressadaptionandvirulenceincochliobolusheterostrophus |