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The Predominance of a Specific Genotype of Cryptococcus neoformans var. Grubii in China and Japan

BACKGROUND: The extensive deployment of molecular genotyping methods is the top reliable keyword to characterize the population genetic structure of C. neoformans in the past decade. However, studies involving genotypic analysis of C. neoformans var. grubii from China and Japan are limited. OBJECTIV...

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Autores principales: Zhu, Jian, Cao, Jiafei, Li, Tingying, Liu, Qi, Kang, Yingqian, Chen, Zhenghong, Meyer, Wieland, Mikami, Yuzuru, Long, Mei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9410922/
https://www.ncbi.nlm.nih.gov/pubmed/36035289
http://dx.doi.org/10.1155/2022/8162096
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author Zhu, Jian
Cao, Jiafei
Li, Tingying
Liu, Qi
Kang, Yingqian
Chen, Zhenghong
Meyer, Wieland
Mikami, Yuzuru
Long, Mei
author_facet Zhu, Jian
Cao, Jiafei
Li, Tingying
Liu, Qi
Kang, Yingqian
Chen, Zhenghong
Meyer, Wieland
Mikami, Yuzuru
Long, Mei
author_sort Zhu, Jian
collection PubMed
description BACKGROUND: The extensive deployment of molecular genotyping methods is the top reliable keyword to characterize the population genetic structure of C. neoformans in the past decade. However, studies involving genotypic analysis of C. neoformans var. grubii from China and Japan are limited. OBJECTIVES: We address this challenge to determine the genotype distribution of C. neoformans var. grubii strains from China and Japan. METHODS: Genotypic analysis of 52 C. neoformans var. grubii isolates was performed using multilocus microsatellite typing (MLMT) based on the sequence analysis of 3 functional genes. In order to place the herein-studied strains into the global picture, 22 strains randomly selected from the 52 strains studied by MLMT were also analyzed by restriction fragment length polymorphism analysis of the orotidine monophosphate pyrophosphorylase gene (URA5-RFLP), M13 PCR-fingerprinting, and multilocus sequence typing (MLST). RESULTS: MLMT classified 46 (88.5%) of the 52 strains as genotype MLMT-17. The high prevalence of the MLMT-17 type was observed for environmental and clinical isolates from China and Japan. URA5-RFLP analysis, M13 PCR-fingerprinting, and MLST showed that most of these belong to the VNI/ST5 (M5) genotype. CONCLUSIONS: Our study suggests the predominance of a specific genotype of C. neoformans var. grubii in China and Japan.
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spelling pubmed-94109222022-08-26 The Predominance of a Specific Genotype of Cryptococcus neoformans var. Grubii in China and Japan Zhu, Jian Cao, Jiafei Li, Tingying Liu, Qi Kang, Yingqian Chen, Zhenghong Meyer, Wieland Mikami, Yuzuru Long, Mei Comput Math Methods Med Research Article BACKGROUND: The extensive deployment of molecular genotyping methods is the top reliable keyword to characterize the population genetic structure of C. neoformans in the past decade. However, studies involving genotypic analysis of C. neoformans var. grubii from China and Japan are limited. OBJECTIVES: We address this challenge to determine the genotype distribution of C. neoformans var. grubii strains from China and Japan. METHODS: Genotypic analysis of 52 C. neoformans var. grubii isolates was performed using multilocus microsatellite typing (MLMT) based on the sequence analysis of 3 functional genes. In order to place the herein-studied strains into the global picture, 22 strains randomly selected from the 52 strains studied by MLMT were also analyzed by restriction fragment length polymorphism analysis of the orotidine monophosphate pyrophosphorylase gene (URA5-RFLP), M13 PCR-fingerprinting, and multilocus sequence typing (MLST). RESULTS: MLMT classified 46 (88.5%) of the 52 strains as genotype MLMT-17. The high prevalence of the MLMT-17 type was observed for environmental and clinical isolates from China and Japan. URA5-RFLP analysis, M13 PCR-fingerprinting, and MLST showed that most of these belong to the VNI/ST5 (M5) genotype. CONCLUSIONS: Our study suggests the predominance of a specific genotype of C. neoformans var. grubii in China and Japan. Hindawi 2022-08-05 /pmc/articles/PMC9410922/ /pubmed/36035289 http://dx.doi.org/10.1155/2022/8162096 Text en Copyright © 2022 Jian Zhu et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhu, Jian
Cao, Jiafei
Li, Tingying
Liu, Qi
Kang, Yingqian
Chen, Zhenghong
Meyer, Wieland
Mikami, Yuzuru
Long, Mei
The Predominance of a Specific Genotype of Cryptococcus neoformans var. Grubii in China and Japan
title The Predominance of a Specific Genotype of Cryptococcus neoformans var. Grubii in China and Japan
title_full The Predominance of a Specific Genotype of Cryptococcus neoformans var. Grubii in China and Japan
title_fullStr The Predominance of a Specific Genotype of Cryptococcus neoformans var. Grubii in China and Japan
title_full_unstemmed The Predominance of a Specific Genotype of Cryptococcus neoformans var. Grubii in China and Japan
title_short The Predominance of a Specific Genotype of Cryptococcus neoformans var. Grubii in China and Japan
title_sort predominance of a specific genotype of cryptococcus neoformans var. grubii in china and japan
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9410922/
https://www.ncbi.nlm.nih.gov/pubmed/36035289
http://dx.doi.org/10.1155/2022/8162096
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