Generation of three human induced pluripotent stem cell sublines (UCLAi005-A, UCLAi005-B and UCLAi005-C) for reproductive science research

We generated three human induced pluripotent stem cell (hiPSC) sublines from human dermal fibroblasts (HDF) (MZT05) generated from a skin biopsy donated from a previously fertile woman. The skin biopsy was broadly consented for generating hiPSC lines for biomedical research, including unique consent...

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Detalles Bibliográficos
Autores principales: Pandolfi, Erica C., Hunt, Timothy J., Goldsmith, Sierra, Hurlbut, Kellie, Silber, Sherman J., Clark, Amander T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9411073/
https://www.ncbi.nlm.nih.gov/pubmed/34130154
http://dx.doi.org/10.1016/j.scr.2021.102409
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author Pandolfi, Erica C.
Hunt, Timothy J.
Goldsmith, Sierra
Hurlbut, Kellie
Silber, Sherman J.
Clark, Amander T.
author_facet Pandolfi, Erica C.
Hunt, Timothy J.
Goldsmith, Sierra
Hurlbut, Kellie
Silber, Sherman J.
Clark, Amander T.
author_sort Pandolfi, Erica C.
collection PubMed
description We generated three human induced pluripotent stem cell (hiPSC) sublines from human dermal fibroblasts (HDF) (MZT05) generated from a skin biopsy donated from a previously fertile woman. The skin biopsy was broadly consented for generating hiPSC lines for biomedical research, including unique consent specifically for studying human fertility, infertility and germ cell differentiation. hiPSCs were reprogrammed using Sendai virus vectors and were subsequently positive for markers of self-renewal. Pluripotency was further verified using PluriTest analysis and in vitro differentiation was tested using Taqman Real-Time PCR assays. These sublines serve as controls for hiPSC research projects aimed at understanding the cell and molecular regulation of female fertility.
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spelling pubmed-94110732022-08-26 Generation of three human induced pluripotent stem cell sublines (UCLAi005-A, UCLAi005-B and UCLAi005-C) for reproductive science research Pandolfi, Erica C. Hunt, Timothy J. Goldsmith, Sierra Hurlbut, Kellie Silber, Sherman J. Clark, Amander T. Stem Cell Res Article We generated three human induced pluripotent stem cell (hiPSC) sublines from human dermal fibroblasts (HDF) (MZT05) generated from a skin biopsy donated from a previously fertile woman. The skin biopsy was broadly consented for generating hiPSC lines for biomedical research, including unique consent specifically for studying human fertility, infertility and germ cell differentiation. hiPSCs were reprogrammed using Sendai virus vectors and were subsequently positive for markers of self-renewal. Pluripotency was further verified using PluriTest analysis and in vitro differentiation was tested using Taqman Real-Time PCR assays. These sublines serve as controls for hiPSC research projects aimed at understanding the cell and molecular regulation of female fertility. 2021-07 2021-06-08 /pmc/articles/PMC9411073/ /pubmed/34130154 http://dx.doi.org/10.1016/j.scr.2021.102409 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Article
Pandolfi, Erica C.
Hunt, Timothy J.
Goldsmith, Sierra
Hurlbut, Kellie
Silber, Sherman J.
Clark, Amander T.
Generation of three human induced pluripotent stem cell sublines (UCLAi005-A, UCLAi005-B and UCLAi005-C) for reproductive science research
title Generation of three human induced pluripotent stem cell sublines (UCLAi005-A, UCLAi005-B and UCLAi005-C) for reproductive science research
title_full Generation of three human induced pluripotent stem cell sublines (UCLAi005-A, UCLAi005-B and UCLAi005-C) for reproductive science research
title_fullStr Generation of three human induced pluripotent stem cell sublines (UCLAi005-A, UCLAi005-B and UCLAi005-C) for reproductive science research
title_full_unstemmed Generation of three human induced pluripotent stem cell sublines (UCLAi005-A, UCLAi005-B and UCLAi005-C) for reproductive science research
title_short Generation of three human induced pluripotent stem cell sublines (UCLAi005-A, UCLAi005-B and UCLAi005-C) for reproductive science research
title_sort generation of three human induced pluripotent stem cell sublines (uclai005-a, uclai005-b and uclai005-c) for reproductive science research
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9411073/
https://www.ncbi.nlm.nih.gov/pubmed/34130154
http://dx.doi.org/10.1016/j.scr.2021.102409
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