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Application of nickel chitosan nanoconjugate as an antifungal agent for combating Fusarium rot of wheat
Agro-researchers are endlessly trying to derive a potential biomolecule having antifungal properties in order to replace the application of synthetic fungicides on agricultural fields. Rot disease often caused by Fusarium solani made severe loss of wheat crops every year. Chitosan and its metallic n...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9411138/ https://www.ncbi.nlm.nih.gov/pubmed/36008575 http://dx.doi.org/10.1038/s41598-022-18670-2 |
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author | Chouhan, Divya Dutta, Ankita Kumar, Anoop Mandal, Palash Choudhuri, Chandrani |
author_facet | Chouhan, Divya Dutta, Ankita Kumar, Anoop Mandal, Palash Choudhuri, Chandrani |
author_sort | Chouhan, Divya |
collection | PubMed |
description | Agro-researchers are endlessly trying to derive a potential biomolecule having antifungal properties in order to replace the application of synthetic fungicides on agricultural fields. Rot disease often caused by Fusarium solani made severe loss of wheat crops every year. Chitosan and its metallic nano-derivatives hold a broad-spectrum antifungal property. Our interdisciplinary study deals with the application of nickel chitosan nanoconjugate (NiCNC) against Fusarium rot of wheat, in comparison with chitosan nanoparticles (CNPs) and commercial fungicide Mancozeb. CNPs and NiCNC were characterized on the basis of UV–Vis spectrophotometry, HR-TEM, FESEM, EDXS and FT-IR. Both CNPs and NiCNC were found effective against the fungal growth, of which NiCNC at 0.04 mg/mL showed complete termination of F. solani grown in suitable medium. Ultrastructural analysis of F. solani conidia treated with NiCNC revealed pronounced damages and disruption of the membrane surface. Fluorescence microscopic study revealed generation of oxidative stress in the fungal system upon NiCNC exposure. Moreover, NiCNC showed reduction in rot disease incidence by 83.33% of wheat seedlings which was further confirmed through the observation of anatomical sections of the stem. NiCNC application helps the seedling to overcome the adverse effect of pathogen, which was evaluated through stress indices attributes. |
format | Online Article Text |
id | pubmed-9411138 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-94111382022-08-27 Application of nickel chitosan nanoconjugate as an antifungal agent for combating Fusarium rot of wheat Chouhan, Divya Dutta, Ankita Kumar, Anoop Mandal, Palash Choudhuri, Chandrani Sci Rep Article Agro-researchers are endlessly trying to derive a potential biomolecule having antifungal properties in order to replace the application of synthetic fungicides on agricultural fields. Rot disease often caused by Fusarium solani made severe loss of wheat crops every year. Chitosan and its metallic nano-derivatives hold a broad-spectrum antifungal property. Our interdisciplinary study deals with the application of nickel chitosan nanoconjugate (NiCNC) against Fusarium rot of wheat, in comparison with chitosan nanoparticles (CNPs) and commercial fungicide Mancozeb. CNPs and NiCNC were characterized on the basis of UV–Vis spectrophotometry, HR-TEM, FESEM, EDXS and FT-IR. Both CNPs and NiCNC were found effective against the fungal growth, of which NiCNC at 0.04 mg/mL showed complete termination of F. solani grown in suitable medium. Ultrastructural analysis of F. solani conidia treated with NiCNC revealed pronounced damages and disruption of the membrane surface. Fluorescence microscopic study revealed generation of oxidative stress in the fungal system upon NiCNC exposure. Moreover, NiCNC showed reduction in rot disease incidence by 83.33% of wheat seedlings which was further confirmed through the observation of anatomical sections of the stem. NiCNC application helps the seedling to overcome the adverse effect of pathogen, which was evaluated through stress indices attributes. Nature Publishing Group UK 2022-08-25 /pmc/articles/PMC9411138/ /pubmed/36008575 http://dx.doi.org/10.1038/s41598-022-18670-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Chouhan, Divya Dutta, Ankita Kumar, Anoop Mandal, Palash Choudhuri, Chandrani Application of nickel chitosan nanoconjugate as an antifungal agent for combating Fusarium rot of wheat |
title | Application of nickel chitosan nanoconjugate as an antifungal agent for combating Fusarium rot of wheat |
title_full | Application of nickel chitosan nanoconjugate as an antifungal agent for combating Fusarium rot of wheat |
title_fullStr | Application of nickel chitosan nanoconjugate as an antifungal agent for combating Fusarium rot of wheat |
title_full_unstemmed | Application of nickel chitosan nanoconjugate as an antifungal agent for combating Fusarium rot of wheat |
title_short | Application of nickel chitosan nanoconjugate as an antifungal agent for combating Fusarium rot of wheat |
title_sort | application of nickel chitosan nanoconjugate as an antifungal agent for combating fusarium rot of wheat |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9411138/ https://www.ncbi.nlm.nih.gov/pubmed/36008575 http://dx.doi.org/10.1038/s41598-022-18670-2 |
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