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SARS-CoV-2 CD8(+) T cell killing assays using replicating viruses and transgenic antigens
The quality of an antigen-specific CD8(+) T cell repertoire is crucial for the clearance of intracellular pathogens, in particular for viral infections. Here, we describe killing assays to determine the function of CD8(+) T cells engineered with SARS-CoV-2-specific T cell receptors in a near-physiol...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9411149/ https://www.ncbi.nlm.nih.gov/pubmed/36152303 http://dx.doi.org/10.1016/j.xpro.2022.101699 |
Sumario: | The quality of an antigen-specific CD8(+) T cell repertoire is crucial for the clearance of intracellular pathogens, in particular for viral infections. Here, we describe killing assays to determine the function of CD8(+) T cells engineered with SARS-CoV-2-specific T cell receptors in a near-physiological system for antigen presentation. We detail the use of target cells either infected with replicating SARS-CoV-2 virus or engineered with SARS-CoV-2 open reading frames. For complete details on the use and execution of this protocol, please refer to Moosmann et al. (2022) and Wagner et al. (2022). |
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