SARS-CoV-2 CD8(+) T cell killing assays using replicating viruses and transgenic antigens

The quality of an antigen-specific CD8(+) T cell repertoire is crucial for the clearance of intracellular pathogens, in particular for viral infections. Here, we describe killing assays to determine the function of CD8(+) T cells engineered with SARS-CoV-2-specific T cell receptors in a near-physiol...

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Autores principales: Mateyka, Laura M., Grass, Vincent, Pichlmair, Andreas, Busch, Dirk H., D’Ippolito, Elvira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9411149/
https://www.ncbi.nlm.nih.gov/pubmed/36152303
http://dx.doi.org/10.1016/j.xpro.2022.101699
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author Mateyka, Laura M.
Grass, Vincent
Pichlmair, Andreas
Busch, Dirk H.
D’Ippolito, Elvira
author_facet Mateyka, Laura M.
Grass, Vincent
Pichlmair, Andreas
Busch, Dirk H.
D’Ippolito, Elvira
author_sort Mateyka, Laura M.
collection PubMed
description The quality of an antigen-specific CD8(+) T cell repertoire is crucial for the clearance of intracellular pathogens, in particular for viral infections. Here, we describe killing assays to determine the function of CD8(+) T cells engineered with SARS-CoV-2-specific T cell receptors in a near-physiological system for antigen presentation. We detail the use of target cells either infected with replicating SARS-CoV-2 virus or engineered with SARS-CoV-2 open reading frames. For complete details on the use and execution of this protocol, please refer to Moosmann et al. (2022) and Wagner et al. (2022).
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spelling pubmed-94111492022-08-26 SARS-CoV-2 CD8(+) T cell killing assays using replicating viruses and transgenic antigens Mateyka, Laura M. Grass, Vincent Pichlmair, Andreas Busch, Dirk H. D’Ippolito, Elvira STAR Protoc Protocol The quality of an antigen-specific CD8(+) T cell repertoire is crucial for the clearance of intracellular pathogens, in particular for viral infections. Here, we describe killing assays to determine the function of CD8(+) T cells engineered with SARS-CoV-2-specific T cell receptors in a near-physiological system for antigen presentation. We detail the use of target cells either infected with replicating SARS-CoV-2 virus or engineered with SARS-CoV-2 open reading frames. For complete details on the use and execution of this protocol, please refer to Moosmann et al. (2022) and Wagner et al. (2022). Elsevier 2022-08-26 /pmc/articles/PMC9411149/ /pubmed/36152303 http://dx.doi.org/10.1016/j.xpro.2022.101699 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Mateyka, Laura M.
Grass, Vincent
Pichlmair, Andreas
Busch, Dirk H.
D’Ippolito, Elvira
SARS-CoV-2 CD8(+) T cell killing assays using replicating viruses and transgenic antigens
title SARS-CoV-2 CD8(+) T cell killing assays using replicating viruses and transgenic antigens
title_full SARS-CoV-2 CD8(+) T cell killing assays using replicating viruses and transgenic antigens
title_fullStr SARS-CoV-2 CD8(+) T cell killing assays using replicating viruses and transgenic antigens
title_full_unstemmed SARS-CoV-2 CD8(+) T cell killing assays using replicating viruses and transgenic antigens
title_short SARS-CoV-2 CD8(+) T cell killing assays using replicating viruses and transgenic antigens
title_sort sars-cov-2 cd8(+) t cell killing assays using replicating viruses and transgenic antigens
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9411149/
https://www.ncbi.nlm.nih.gov/pubmed/36152303
http://dx.doi.org/10.1016/j.xpro.2022.101699
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