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Osmotic stress tolerance and transcriptome analysis of Gluconobacter oxydans to extra-high titers of glucose

Gluconobacter oxydans has been widely acknowledged as an ideal strain for industrial bio-oxidations with fantastic yield and productivity. Even 600 g/L xylose can be catalyzed efficiently in a sealed and compressed oxygen-supplying bioreactor. Therefore, the present study seeks to explore the osmoti...

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Autores principales: Liu, Xinlu, Wang, Zhiwei, Xiao, Jianjian, Zhou, Xin, Xu, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9412170/
https://www.ncbi.nlm.nih.gov/pubmed/36033857
http://dx.doi.org/10.3389/fmicb.2022.977024
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author Liu, Xinlu
Wang, Zhiwei
Xiao, Jianjian
Zhou, Xin
Xu, Yong
author_facet Liu, Xinlu
Wang, Zhiwei
Xiao, Jianjian
Zhou, Xin
Xu, Yong
author_sort Liu, Xinlu
collection PubMed
description Gluconobacter oxydans has been widely acknowledged as an ideal strain for industrial bio-oxidations with fantastic yield and productivity. Even 600 g/L xylose can be catalyzed efficiently in a sealed and compressed oxygen-supplying bioreactor. Therefore, the present study seeks to explore the osmotic stress tolerance against extra-high titer of representative lignocellulosic sugars like glucose. Gluconobacter oxydans can well adapted and fermented with initial 600 g/L glucose, exhibiting the highest bio-tolerance in prokaryotic strains and the comparability to the eukaryotic strain of Saccharomyces cerevisiae. 1,432 differentially expressed genes corresponding to osmotic pressure are detected through transcriptome analysis, involving several genes related to the probable compatible solutes (trehalose and arginine). Gluconobacter oxydans obtains more energy by enhancing the substrate-level phosphorylation, resulting in the increased glucose consumption rate after fermentation adaption phase. This study will provide insights into further investigation of biological tolerance and response to extra-high titers of glucose of G. oxydans.
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spelling pubmed-94121702022-08-27 Osmotic stress tolerance and transcriptome analysis of Gluconobacter oxydans to extra-high titers of glucose Liu, Xinlu Wang, Zhiwei Xiao, Jianjian Zhou, Xin Xu, Yong Front Microbiol Microbiology Gluconobacter oxydans has been widely acknowledged as an ideal strain for industrial bio-oxidations with fantastic yield and productivity. Even 600 g/L xylose can be catalyzed efficiently in a sealed and compressed oxygen-supplying bioreactor. Therefore, the present study seeks to explore the osmotic stress tolerance against extra-high titer of representative lignocellulosic sugars like glucose. Gluconobacter oxydans can well adapted and fermented with initial 600 g/L glucose, exhibiting the highest bio-tolerance in prokaryotic strains and the comparability to the eukaryotic strain of Saccharomyces cerevisiae. 1,432 differentially expressed genes corresponding to osmotic pressure are detected through transcriptome analysis, involving several genes related to the probable compatible solutes (trehalose and arginine). Gluconobacter oxydans obtains more energy by enhancing the substrate-level phosphorylation, resulting in the increased glucose consumption rate after fermentation adaption phase. This study will provide insights into further investigation of biological tolerance and response to extra-high titers of glucose of G. oxydans. Frontiers Media S.A. 2022-08-12 /pmc/articles/PMC9412170/ /pubmed/36033857 http://dx.doi.org/10.3389/fmicb.2022.977024 Text en Copyright © 2022 Liu, Wang, Xiao, Zhou and Xu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Liu, Xinlu
Wang, Zhiwei
Xiao, Jianjian
Zhou, Xin
Xu, Yong
Osmotic stress tolerance and transcriptome analysis of Gluconobacter oxydans to extra-high titers of glucose
title Osmotic stress tolerance and transcriptome analysis of Gluconobacter oxydans to extra-high titers of glucose
title_full Osmotic stress tolerance and transcriptome analysis of Gluconobacter oxydans to extra-high titers of glucose
title_fullStr Osmotic stress tolerance and transcriptome analysis of Gluconobacter oxydans to extra-high titers of glucose
title_full_unstemmed Osmotic stress tolerance and transcriptome analysis of Gluconobacter oxydans to extra-high titers of glucose
title_short Osmotic stress tolerance and transcriptome analysis of Gluconobacter oxydans to extra-high titers of glucose
title_sort osmotic stress tolerance and transcriptome analysis of gluconobacter oxydans to extra-high titers of glucose
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9412170/
https://www.ncbi.nlm.nih.gov/pubmed/36033857
http://dx.doi.org/10.3389/fmicb.2022.977024
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