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Osmotic stress tolerance and transcriptome analysis of Gluconobacter oxydans to extra-high titers of glucose
Gluconobacter oxydans has been widely acknowledged as an ideal strain for industrial bio-oxidations with fantastic yield and productivity. Even 600 g/L xylose can be catalyzed efficiently in a sealed and compressed oxygen-supplying bioreactor. Therefore, the present study seeks to explore the osmoti...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9412170/ https://www.ncbi.nlm.nih.gov/pubmed/36033857 http://dx.doi.org/10.3389/fmicb.2022.977024 |
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author | Liu, Xinlu Wang, Zhiwei Xiao, Jianjian Zhou, Xin Xu, Yong |
author_facet | Liu, Xinlu Wang, Zhiwei Xiao, Jianjian Zhou, Xin Xu, Yong |
author_sort | Liu, Xinlu |
collection | PubMed |
description | Gluconobacter oxydans has been widely acknowledged as an ideal strain for industrial bio-oxidations with fantastic yield and productivity. Even 600 g/L xylose can be catalyzed efficiently in a sealed and compressed oxygen-supplying bioreactor. Therefore, the present study seeks to explore the osmotic stress tolerance against extra-high titer of representative lignocellulosic sugars like glucose. Gluconobacter oxydans can well adapted and fermented with initial 600 g/L glucose, exhibiting the highest bio-tolerance in prokaryotic strains and the comparability to the eukaryotic strain of Saccharomyces cerevisiae. 1,432 differentially expressed genes corresponding to osmotic pressure are detected through transcriptome analysis, involving several genes related to the probable compatible solutes (trehalose and arginine). Gluconobacter oxydans obtains more energy by enhancing the substrate-level phosphorylation, resulting in the increased glucose consumption rate after fermentation adaption phase. This study will provide insights into further investigation of biological tolerance and response to extra-high titers of glucose of G. oxydans. |
format | Online Article Text |
id | pubmed-9412170 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94121702022-08-27 Osmotic stress tolerance and transcriptome analysis of Gluconobacter oxydans to extra-high titers of glucose Liu, Xinlu Wang, Zhiwei Xiao, Jianjian Zhou, Xin Xu, Yong Front Microbiol Microbiology Gluconobacter oxydans has been widely acknowledged as an ideal strain for industrial bio-oxidations with fantastic yield and productivity. Even 600 g/L xylose can be catalyzed efficiently in a sealed and compressed oxygen-supplying bioreactor. Therefore, the present study seeks to explore the osmotic stress tolerance against extra-high titer of representative lignocellulosic sugars like glucose. Gluconobacter oxydans can well adapted and fermented with initial 600 g/L glucose, exhibiting the highest bio-tolerance in prokaryotic strains and the comparability to the eukaryotic strain of Saccharomyces cerevisiae. 1,432 differentially expressed genes corresponding to osmotic pressure are detected through transcriptome analysis, involving several genes related to the probable compatible solutes (trehalose and arginine). Gluconobacter oxydans obtains more energy by enhancing the substrate-level phosphorylation, resulting in the increased glucose consumption rate after fermentation adaption phase. This study will provide insights into further investigation of biological tolerance and response to extra-high titers of glucose of G. oxydans. Frontiers Media S.A. 2022-08-12 /pmc/articles/PMC9412170/ /pubmed/36033857 http://dx.doi.org/10.3389/fmicb.2022.977024 Text en Copyright © 2022 Liu, Wang, Xiao, Zhou and Xu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Liu, Xinlu Wang, Zhiwei Xiao, Jianjian Zhou, Xin Xu, Yong Osmotic stress tolerance and transcriptome analysis of Gluconobacter oxydans to extra-high titers of glucose |
title | Osmotic stress tolerance and transcriptome analysis of Gluconobacter oxydans to extra-high titers of glucose |
title_full | Osmotic stress tolerance and transcriptome analysis of Gluconobacter oxydans to extra-high titers of glucose |
title_fullStr | Osmotic stress tolerance and transcriptome analysis of Gluconobacter oxydans to extra-high titers of glucose |
title_full_unstemmed | Osmotic stress tolerance and transcriptome analysis of Gluconobacter oxydans to extra-high titers of glucose |
title_short | Osmotic stress tolerance and transcriptome analysis of Gluconobacter oxydans to extra-high titers of glucose |
title_sort | osmotic stress tolerance and transcriptome analysis of gluconobacter oxydans to extra-high titers of glucose |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9412170/ https://www.ncbi.nlm.nih.gov/pubmed/36033857 http://dx.doi.org/10.3389/fmicb.2022.977024 |
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