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Complete Genomic RNA Sequence of Tuberose Mild Mosaic Virus and Tuberose Mild Mottle Virus Acquired by High-Throughput Sequencing

Tuberose (Polianthes tuberosa) is an ornamental flowering crop of the Amaryllidaceae family. Tuberose mild mosaic virus (TuMMV) and tuberose mild mottle virus (TuMMoV), members of the genus Potyvirus, are ubiquitously distributed in most tuberose growing countries worldwide with low biological incid...

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Autores principales: Prajapati, Malyaj R., Manav, Aakansha, Singhal, Pankhuri, Sidharthan, Venkidusamy K., Sirohi, Ujjwal, Kumar, Mukesh, Bharti, Mahesh Kumar, Singh, Jitender, Kumar, Pankaj, Kumar, Ravindra, Prakash, Satya, Baranwal, Virendra Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9412269/
https://www.ncbi.nlm.nih.gov/pubmed/36014982
http://dx.doi.org/10.3390/pathogens11080861
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author Prajapati, Malyaj R.
Manav, Aakansha
Singhal, Pankhuri
Sidharthan, Venkidusamy K.
Sirohi, Ujjwal
Kumar, Mukesh
Bharti, Mahesh Kumar
Singh, Jitender
Kumar, Pankaj
Kumar, Ravindra
Prakash, Satya
Baranwal, Virendra Kumar
author_facet Prajapati, Malyaj R.
Manav, Aakansha
Singhal, Pankhuri
Sidharthan, Venkidusamy K.
Sirohi, Ujjwal
Kumar, Mukesh
Bharti, Mahesh Kumar
Singh, Jitender
Kumar, Pankaj
Kumar, Ravindra
Prakash, Satya
Baranwal, Virendra Kumar
author_sort Prajapati, Malyaj R.
collection PubMed
description Tuberose (Polianthes tuberosa) is an ornamental flowering crop of the Amaryllidaceae family. Tuberose mild mosaic virus (TuMMV) and tuberose mild mottle virus (TuMMoV), members of the genus Potyvirus, are ubiquitously distributed in most tuberose growing countries worldwide with low biological incidence. Here, we report the first coding-complete genomic RNA of TuMMV and TuMMoV obtained through high-throughput sequencing (HTS) and further, the presence of both the viruses were confirmed using virus-specific primers in RT-PCR assays. Excluding the poly (A) tail, the coding-complete genomic RNA of TuMMV and TuMMoV was 9485 and 9462 nucleotides (nts) in length, respectively, and contained a single large open reading frame (ORF). Polyprotein encoded by both the viral genomes contained nine putative cleavage sites. BLASTn analysis of TuMMV and TuMMoV genomes showed 72.40–76.80% and 67.95–77% nucleotide sequence similarities, respectively, with the existing potyviral sequences. Phylogenetic analysis based on genome sequences showed that TuMMV and TuMMoV clustered in a distinct clade to other potyviruses. Further studies are required to understand the mechanism of symptom development, distribution, genetic variability, and their possible threat to tuberose production in India.
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spelling pubmed-94122692022-08-27 Complete Genomic RNA Sequence of Tuberose Mild Mosaic Virus and Tuberose Mild Mottle Virus Acquired by High-Throughput Sequencing Prajapati, Malyaj R. Manav, Aakansha Singhal, Pankhuri Sidharthan, Venkidusamy K. Sirohi, Ujjwal Kumar, Mukesh Bharti, Mahesh Kumar Singh, Jitender Kumar, Pankaj Kumar, Ravindra Prakash, Satya Baranwal, Virendra Kumar Pathogens Article Tuberose (Polianthes tuberosa) is an ornamental flowering crop of the Amaryllidaceae family. Tuberose mild mosaic virus (TuMMV) and tuberose mild mottle virus (TuMMoV), members of the genus Potyvirus, are ubiquitously distributed in most tuberose growing countries worldwide with low biological incidence. Here, we report the first coding-complete genomic RNA of TuMMV and TuMMoV obtained through high-throughput sequencing (HTS) and further, the presence of both the viruses were confirmed using virus-specific primers in RT-PCR assays. Excluding the poly (A) tail, the coding-complete genomic RNA of TuMMV and TuMMoV was 9485 and 9462 nucleotides (nts) in length, respectively, and contained a single large open reading frame (ORF). Polyprotein encoded by both the viral genomes contained nine putative cleavage sites. BLASTn analysis of TuMMV and TuMMoV genomes showed 72.40–76.80% and 67.95–77% nucleotide sequence similarities, respectively, with the existing potyviral sequences. Phylogenetic analysis based on genome sequences showed that TuMMV and TuMMoV clustered in a distinct clade to other potyviruses. Further studies are required to understand the mechanism of symptom development, distribution, genetic variability, and their possible threat to tuberose production in India. MDPI 2022-07-30 /pmc/articles/PMC9412269/ /pubmed/36014982 http://dx.doi.org/10.3390/pathogens11080861 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Prajapati, Malyaj R.
Manav, Aakansha
Singhal, Pankhuri
Sidharthan, Venkidusamy K.
Sirohi, Ujjwal
Kumar, Mukesh
Bharti, Mahesh Kumar
Singh, Jitender
Kumar, Pankaj
Kumar, Ravindra
Prakash, Satya
Baranwal, Virendra Kumar
Complete Genomic RNA Sequence of Tuberose Mild Mosaic Virus and Tuberose Mild Mottle Virus Acquired by High-Throughput Sequencing
title Complete Genomic RNA Sequence of Tuberose Mild Mosaic Virus and Tuberose Mild Mottle Virus Acquired by High-Throughput Sequencing
title_full Complete Genomic RNA Sequence of Tuberose Mild Mosaic Virus and Tuberose Mild Mottle Virus Acquired by High-Throughput Sequencing
title_fullStr Complete Genomic RNA Sequence of Tuberose Mild Mosaic Virus and Tuberose Mild Mottle Virus Acquired by High-Throughput Sequencing
title_full_unstemmed Complete Genomic RNA Sequence of Tuberose Mild Mosaic Virus and Tuberose Mild Mottle Virus Acquired by High-Throughput Sequencing
title_short Complete Genomic RNA Sequence of Tuberose Mild Mosaic Virus and Tuberose Mild Mottle Virus Acquired by High-Throughput Sequencing
title_sort complete genomic rna sequence of tuberose mild mosaic virus and tuberose mild mottle virus acquired by high-throughput sequencing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9412269/
https://www.ncbi.nlm.nih.gov/pubmed/36014982
http://dx.doi.org/10.3390/pathogens11080861
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