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Identification and Spatial Distribution of Bioactive Compounds in Seeds Vigna unguiculata (L.) Walp. by Laser Microscopy and Tandem Mass Spectrometry

The research presents a comparative metabolomic study of extracts of Vigna unguiculata seed samples from the collection of the N.I. Vavilov All-Russian Institute of Plant Genetic Resources. Analyzed samples related to different areas of use in agricultural production, belonging to different cultivar...

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Autores principales: Razgonova, Mayya P., Burlyaeva, Marina O., Zinchenko, Yulia N., Krylova, Ekaterina A., Chunikhina, Olga A., Ivanova, Natalia M., Zakharenko, Alexander M., Golokhvast, Kirill S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9412441/
https://www.ncbi.nlm.nih.gov/pubmed/36015450
http://dx.doi.org/10.3390/plants11162147
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author Razgonova, Mayya P.
Burlyaeva, Marina O.
Zinchenko, Yulia N.
Krylova, Ekaterina A.
Chunikhina, Olga A.
Ivanova, Natalia M.
Zakharenko, Alexander M.
Golokhvast, Kirill S.
author_facet Razgonova, Mayya P.
Burlyaeva, Marina O.
Zinchenko, Yulia N.
Krylova, Ekaterina A.
Chunikhina, Olga A.
Ivanova, Natalia M.
Zakharenko, Alexander M.
Golokhvast, Kirill S.
author_sort Razgonova, Mayya P.
collection PubMed
description The research presents a comparative metabolomic study of extracts of Vigna unguiculata seed samples from the collection of the N.I. Vavilov All-Russian Institute of Plant Genetic Resources. Analyzed samples related to different areas of use in agricultural production, belonging to different cultivar groups sesquipedalis (vegetable accessions) and unguiculata (grain accessions). Metabolome analysis was performed by liquid chromatography combined with ion trap mass spectrometry. Substances were localized in seeds using confocal and laser microscopy. As a result, 49 bioactive compounds were identified: flavonols, flavones, flavan-3-ols, anthocyanidin, phenolic acids, amino acids, monocarboxylic acids, aminobenzoic acids, fatty acids, lignans, carotenoid, sapogenins, steroids, etc. Steroidal alkaloids were identified in V. unguiculata seeds for the first time. The seed coat (palisade epidermis and parenchyma) is the richest in phenolic compounds. Comparison of seeds of varieties of different directions of use in terms of the number of bioactive substances identified revealed a significant superiority of vegetable accessions over grain ones in this indicator, 36 compounds were found in samples from cultivar group sesquipedalis, and 24 in unguiculata. The greatest variety of bioactive compounds was found in the vegetable accession k-640 from China.
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spelling pubmed-94124412022-08-27 Identification and Spatial Distribution of Bioactive Compounds in Seeds Vigna unguiculata (L.) Walp. by Laser Microscopy and Tandem Mass Spectrometry Razgonova, Mayya P. Burlyaeva, Marina O. Zinchenko, Yulia N. Krylova, Ekaterina A. Chunikhina, Olga A. Ivanova, Natalia M. Zakharenko, Alexander M. Golokhvast, Kirill S. Plants (Basel) Article The research presents a comparative metabolomic study of extracts of Vigna unguiculata seed samples from the collection of the N.I. Vavilov All-Russian Institute of Plant Genetic Resources. Analyzed samples related to different areas of use in agricultural production, belonging to different cultivar groups sesquipedalis (vegetable accessions) and unguiculata (grain accessions). Metabolome analysis was performed by liquid chromatography combined with ion trap mass spectrometry. Substances were localized in seeds using confocal and laser microscopy. As a result, 49 bioactive compounds were identified: flavonols, flavones, flavan-3-ols, anthocyanidin, phenolic acids, amino acids, monocarboxylic acids, aminobenzoic acids, fatty acids, lignans, carotenoid, sapogenins, steroids, etc. Steroidal alkaloids were identified in V. unguiculata seeds for the first time. The seed coat (palisade epidermis and parenchyma) is the richest in phenolic compounds. Comparison of seeds of varieties of different directions of use in terms of the number of bioactive substances identified revealed a significant superiority of vegetable accessions over grain ones in this indicator, 36 compounds were found in samples from cultivar group sesquipedalis, and 24 in unguiculata. The greatest variety of bioactive compounds was found in the vegetable accession k-640 from China. MDPI 2022-08-18 /pmc/articles/PMC9412441/ /pubmed/36015450 http://dx.doi.org/10.3390/plants11162147 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Razgonova, Mayya P.
Burlyaeva, Marina O.
Zinchenko, Yulia N.
Krylova, Ekaterina A.
Chunikhina, Olga A.
Ivanova, Natalia M.
Zakharenko, Alexander M.
Golokhvast, Kirill S.
Identification and Spatial Distribution of Bioactive Compounds in Seeds Vigna unguiculata (L.) Walp. by Laser Microscopy and Tandem Mass Spectrometry
title Identification and Spatial Distribution of Bioactive Compounds in Seeds Vigna unguiculata (L.) Walp. by Laser Microscopy and Tandem Mass Spectrometry
title_full Identification and Spatial Distribution of Bioactive Compounds in Seeds Vigna unguiculata (L.) Walp. by Laser Microscopy and Tandem Mass Spectrometry
title_fullStr Identification and Spatial Distribution of Bioactive Compounds in Seeds Vigna unguiculata (L.) Walp. by Laser Microscopy and Tandem Mass Spectrometry
title_full_unstemmed Identification and Spatial Distribution of Bioactive Compounds in Seeds Vigna unguiculata (L.) Walp. by Laser Microscopy and Tandem Mass Spectrometry
title_short Identification and Spatial Distribution of Bioactive Compounds in Seeds Vigna unguiculata (L.) Walp. by Laser Microscopy and Tandem Mass Spectrometry
title_sort identification and spatial distribution of bioactive compounds in seeds vigna unguiculata (l.) walp. by laser microscopy and tandem mass spectrometry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9412441/
https://www.ncbi.nlm.nih.gov/pubmed/36015450
http://dx.doi.org/10.3390/plants11162147
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