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AG129 Mice as a Comprehensive Model for the Experimental Assessment of Mosquito Vector Competence for Arboviruses

Arboviruses (an acronym for “arthropod-borne virus”), such as dengue, yellow fever, Zika, and Chikungunya, are important human pathogens transmitted by mosquitoes. These viruses impose a growing burden on public health. Despite laboratory mice having been used for decades for understanding the basic...

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Autores principales: Baldon, Lívia V. R., de Mendonça, Silvana F., Ferreira, Flávia V., Rezende, Fernanda O., Amadou, Siad C. G., Leite, Thiago H. J. F., Rocha, Marcele N., Marques, João T., Moreira, Luciano A., Ferreira, Alvaro G. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9412449/
https://www.ncbi.nlm.nih.gov/pubmed/36015000
http://dx.doi.org/10.3390/pathogens11080879
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author Baldon, Lívia V. R.
de Mendonça, Silvana F.
Ferreira, Flávia V.
Rezende, Fernanda O.
Amadou, Siad C. G.
Leite, Thiago H. J. F.
Rocha, Marcele N.
Marques, João T.
Moreira, Luciano A.
Ferreira, Alvaro G. A.
author_facet Baldon, Lívia V. R.
de Mendonça, Silvana F.
Ferreira, Flávia V.
Rezende, Fernanda O.
Amadou, Siad C. G.
Leite, Thiago H. J. F.
Rocha, Marcele N.
Marques, João T.
Moreira, Luciano A.
Ferreira, Alvaro G. A.
author_sort Baldon, Lívia V. R.
collection PubMed
description Arboviruses (an acronym for “arthropod-borne virus”), such as dengue, yellow fever, Zika, and Chikungunya, are important human pathogens transmitted by mosquitoes. These viruses impose a growing burden on public health. Despite laboratory mice having been used for decades for understanding the basic biological phenomena of these viruses, it was only recently that researchers started to develop immunocompromised animals to study the pathogenesis of arboviruses and their transmission in a way that parallels natural cycles. Here, we show that the AG129 mouse (IFN α/β/γ R(−/−)) is a suitable and comprehensive vertebrate model for studying the mosquito vector competence for the major arboviruses of medical importance, namely the dengue virus (DENV), yellow fever virus (YFV), Zika virus (ZIKV), Mayaro virus (MAYV), and Chikungunya virus (CHIKV). We found that, after intraperitoneal injection, AG129 mice developed a transient viremia lasting several days, peaking on day two or three post infection, for all five arboviruses tested in this study. Furthermore, we found that the observed viremia was ample enough to infect Aedes aegypti during a blood meal from the AG129 infected mice. Finally, we demonstrated that infected mosquitoes could transmit each of the tested arboviruses back to naïve AG129 mice, completing a full transmission cycle of these vector-borne viruses. Together, our data show that A129 mice are a simple and comprehensive vertebrate model for studies of vector competence, as well as investigations into other aspects of mosquito biology that can affect virus–host interactions.
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spelling pubmed-94124492022-08-27 AG129 Mice as a Comprehensive Model for the Experimental Assessment of Mosquito Vector Competence for Arboviruses Baldon, Lívia V. R. de Mendonça, Silvana F. Ferreira, Flávia V. Rezende, Fernanda O. Amadou, Siad C. G. Leite, Thiago H. J. F. Rocha, Marcele N. Marques, João T. Moreira, Luciano A. Ferreira, Alvaro G. A. Pathogens Article Arboviruses (an acronym for “arthropod-borne virus”), such as dengue, yellow fever, Zika, and Chikungunya, are important human pathogens transmitted by mosquitoes. These viruses impose a growing burden on public health. Despite laboratory mice having been used for decades for understanding the basic biological phenomena of these viruses, it was only recently that researchers started to develop immunocompromised animals to study the pathogenesis of arboviruses and their transmission in a way that parallels natural cycles. Here, we show that the AG129 mouse (IFN α/β/γ R(−/−)) is a suitable and comprehensive vertebrate model for studying the mosquito vector competence for the major arboviruses of medical importance, namely the dengue virus (DENV), yellow fever virus (YFV), Zika virus (ZIKV), Mayaro virus (MAYV), and Chikungunya virus (CHIKV). We found that, after intraperitoneal injection, AG129 mice developed a transient viremia lasting several days, peaking on day two or three post infection, for all five arboviruses tested in this study. Furthermore, we found that the observed viremia was ample enough to infect Aedes aegypti during a blood meal from the AG129 infected mice. Finally, we demonstrated that infected mosquitoes could transmit each of the tested arboviruses back to naïve AG129 mice, completing a full transmission cycle of these vector-borne viruses. Together, our data show that A129 mice are a simple and comprehensive vertebrate model for studies of vector competence, as well as investigations into other aspects of mosquito biology that can affect virus–host interactions. MDPI 2022-08-03 /pmc/articles/PMC9412449/ /pubmed/36015000 http://dx.doi.org/10.3390/pathogens11080879 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Baldon, Lívia V. R.
de Mendonça, Silvana F.
Ferreira, Flávia V.
Rezende, Fernanda O.
Amadou, Siad C. G.
Leite, Thiago H. J. F.
Rocha, Marcele N.
Marques, João T.
Moreira, Luciano A.
Ferreira, Alvaro G. A.
AG129 Mice as a Comprehensive Model for the Experimental Assessment of Mosquito Vector Competence for Arboviruses
title AG129 Mice as a Comprehensive Model for the Experimental Assessment of Mosquito Vector Competence for Arboviruses
title_full AG129 Mice as a Comprehensive Model for the Experimental Assessment of Mosquito Vector Competence for Arboviruses
title_fullStr AG129 Mice as a Comprehensive Model for the Experimental Assessment of Mosquito Vector Competence for Arboviruses
title_full_unstemmed AG129 Mice as a Comprehensive Model for the Experimental Assessment of Mosquito Vector Competence for Arboviruses
title_short AG129 Mice as a Comprehensive Model for the Experimental Assessment of Mosquito Vector Competence for Arboviruses
title_sort ag129 mice as a comprehensive model for the experimental assessment of mosquito vector competence for arboviruses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9412449/
https://www.ncbi.nlm.nih.gov/pubmed/36015000
http://dx.doi.org/10.3390/pathogens11080879
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