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Botryosphaeria Dieback (Lasiodiplodia viticola): An Imminent Emerging Threat to the Moroccan Vineyards

A decline of various grapevines (Vitis vinifera L.) in the province of Doukkala in Morocco was observed in 2021. The causal pathogen was identified as Lasiodiplodia viticola based on morphological characteristics and phylogenetic analysis of the internal transcribed region (ITS), the β-tubulin gene...

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Autores principales: Kenfaoui, Jihane, Lahlali, Rachid, Mennani, Mohammed, Radouane, Nabil, Goura, Khadija, El Hamss, Hajar, El Ghadraoui, Lahsen, Fontaine, Florence, Tahiri, Abdessalem, Barka, Essaid Ait, Amiri, Said
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9412503/
https://www.ncbi.nlm.nih.gov/pubmed/36015470
http://dx.doi.org/10.3390/plants11162167
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author Kenfaoui, Jihane
Lahlali, Rachid
Mennani, Mohammed
Radouane, Nabil
Goura, Khadija
El Hamss, Hajar
El Ghadraoui, Lahsen
Fontaine, Florence
Tahiri, Abdessalem
Barka, Essaid Ait
Amiri, Said
author_facet Kenfaoui, Jihane
Lahlali, Rachid
Mennani, Mohammed
Radouane, Nabil
Goura, Khadija
El Hamss, Hajar
El Ghadraoui, Lahsen
Fontaine, Florence
Tahiri, Abdessalem
Barka, Essaid Ait
Amiri, Said
author_sort Kenfaoui, Jihane
collection PubMed
description A decline of various grapevines (Vitis vinifera L.) in the province of Doukkala in Morocco was observed in 2021. The causal pathogen was identified as Lasiodiplodia viticola based on morphological characteristics and phylogenetic analysis of the internal transcribed region (ITS), the β-tubulin gene (TUB) and calmodulin (cmdA). Koch’s postulates were confirmed by successful re-isolation of L. viticola from plants inoculated with the pathogen under controlled conditions. The disease was shown to be prevalent in Bni Hilal (71.43%), Laamria (60%), and Boulaouane (40%) districts, but was quasi-absent in Lmechrek. To understand the dominance of L. viticola as one of the grapevine trunk pathogens, effects of temperature (10–40 °C) and pH (pH 3–pH 12) on growth and sporulation were investigated. The species were able to grow in a range of temperatures ranging from 15 to 40°C and showed a higher growth rate at 35 °C. The fungus were also characterized by a broad optimum pH ranging between 3–12. This study is the first report dealing with L. viticola associated with grapevine trunk diseases in Morocco. Additional studies are therefore required to understand the high occurrence of this disease in vineyards, which is likely due to climate changes. A good understanding of this complex disease might help to develop a reliable and sustainable preventive control strategy.
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spelling pubmed-94125032022-08-27 Botryosphaeria Dieback (Lasiodiplodia viticola): An Imminent Emerging Threat to the Moroccan Vineyards Kenfaoui, Jihane Lahlali, Rachid Mennani, Mohammed Radouane, Nabil Goura, Khadija El Hamss, Hajar El Ghadraoui, Lahsen Fontaine, Florence Tahiri, Abdessalem Barka, Essaid Ait Amiri, Said Plants (Basel) Article A decline of various grapevines (Vitis vinifera L.) in the province of Doukkala in Morocco was observed in 2021. The causal pathogen was identified as Lasiodiplodia viticola based on morphological characteristics and phylogenetic analysis of the internal transcribed region (ITS), the β-tubulin gene (TUB) and calmodulin (cmdA). Koch’s postulates were confirmed by successful re-isolation of L. viticola from plants inoculated with the pathogen under controlled conditions. The disease was shown to be prevalent in Bni Hilal (71.43%), Laamria (60%), and Boulaouane (40%) districts, but was quasi-absent in Lmechrek. To understand the dominance of L. viticola as one of the grapevine trunk pathogens, effects of temperature (10–40 °C) and pH (pH 3–pH 12) on growth and sporulation were investigated. The species were able to grow in a range of temperatures ranging from 15 to 40°C and showed a higher growth rate at 35 °C. The fungus were also characterized by a broad optimum pH ranging between 3–12. This study is the first report dealing with L. viticola associated with grapevine trunk diseases in Morocco. Additional studies are therefore required to understand the high occurrence of this disease in vineyards, which is likely due to climate changes. A good understanding of this complex disease might help to develop a reliable and sustainable preventive control strategy. MDPI 2022-08-21 /pmc/articles/PMC9412503/ /pubmed/36015470 http://dx.doi.org/10.3390/plants11162167 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kenfaoui, Jihane
Lahlali, Rachid
Mennani, Mohammed
Radouane, Nabil
Goura, Khadija
El Hamss, Hajar
El Ghadraoui, Lahsen
Fontaine, Florence
Tahiri, Abdessalem
Barka, Essaid Ait
Amiri, Said
Botryosphaeria Dieback (Lasiodiplodia viticola): An Imminent Emerging Threat to the Moroccan Vineyards
title Botryosphaeria Dieback (Lasiodiplodia viticola): An Imminent Emerging Threat to the Moroccan Vineyards
title_full Botryosphaeria Dieback (Lasiodiplodia viticola): An Imminent Emerging Threat to the Moroccan Vineyards
title_fullStr Botryosphaeria Dieback (Lasiodiplodia viticola): An Imminent Emerging Threat to the Moroccan Vineyards
title_full_unstemmed Botryosphaeria Dieback (Lasiodiplodia viticola): An Imminent Emerging Threat to the Moroccan Vineyards
title_short Botryosphaeria Dieback (Lasiodiplodia viticola): An Imminent Emerging Threat to the Moroccan Vineyards
title_sort botryosphaeria dieback (lasiodiplodia viticola): an imminent emerging threat to the moroccan vineyards
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9412503/
https://www.ncbi.nlm.nih.gov/pubmed/36015470
http://dx.doi.org/10.3390/plants11162167
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