Cargando…

A Portable Continuous-Flow Polymerase Chain Reaction Chip Device Integrated with Arduino Boards for Detecting Colla corii asini

Food security is a significant issue in modern society. Because morphological characters are not reliable enough to distinguish authentic traditional Chinese medicines, it is essential to establish an effective and applicable method to identify them to protect people’s health. Due to the expensive c...

Descripción completa

Detalles Bibliográficos
Autores principales: Sheu, Shyang-Chwen, Song, Yi-Syuan, Chen, Jyh-Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9412515/
https://www.ncbi.nlm.nih.gov/pubmed/36014212
http://dx.doi.org/10.3390/mi13081289
_version_ 1784775513129091072
author Sheu, Shyang-Chwen
Song, Yi-Syuan
Chen, Jyh-Jian
author_facet Sheu, Shyang-Chwen
Song, Yi-Syuan
Chen, Jyh-Jian
author_sort Sheu, Shyang-Chwen
collection PubMed
description Food security is a significant issue in modern society. Because morphological characters are not reliable enough to distinguish authentic traditional Chinese medicines, it is essential to establish an effective and applicable method to identify them to protect people’s health. Due to the expensive cost of the manufacturing process and the large volume of the analytical system, the need to build a portable and cheap device is urgent. This work describes the development of a portable nucleic acid amplification device integrated with thermal control and liquid pumping connecting to Arduino boards. We present a novel microfluidic polymerase chain reaction (PCR) chip with symmetric isothermal zones. The total chip volume is small, and only one Arduino board is needed for thermal control. We assemble a miniaturized liquid pump and program an Arduino file to push the sample mixture into the chip to implement the PCR process. In the proposed operation, the Nusselt number of the sample flow is less than one, and the heat transfer is conduction only. Then we can ensure temperature uniformity in specific reaction regions. A Colla corii asini DNA segment of 200 bp is amplified to evaluate the PCR performance under the various operational parameters. The initial concentration for accomplishing the PCR process is at least 20 ng/μL at the flow rate of 0.4 μL/min in the portable continuous flow PCR (CFPCR) device. To our knowledge, our group is the first to introduce Arduino boards into the heat control and sample pumping modules for a CFPCR device.
format Online
Article
Text
id pubmed-9412515
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-94125152022-08-27 A Portable Continuous-Flow Polymerase Chain Reaction Chip Device Integrated with Arduino Boards for Detecting Colla corii asini Sheu, Shyang-Chwen Song, Yi-Syuan Chen, Jyh-Jian Micromachines (Basel) Article Food security is a significant issue in modern society. Because morphological characters are not reliable enough to distinguish authentic traditional Chinese medicines, it is essential to establish an effective and applicable method to identify them to protect people’s health. Due to the expensive cost of the manufacturing process and the large volume of the analytical system, the need to build a portable and cheap device is urgent. This work describes the development of a portable nucleic acid amplification device integrated with thermal control and liquid pumping connecting to Arduino boards. We present a novel microfluidic polymerase chain reaction (PCR) chip with symmetric isothermal zones. The total chip volume is small, and only one Arduino board is needed for thermal control. We assemble a miniaturized liquid pump and program an Arduino file to push the sample mixture into the chip to implement the PCR process. In the proposed operation, the Nusselt number of the sample flow is less than one, and the heat transfer is conduction only. Then we can ensure temperature uniformity in specific reaction regions. A Colla corii asini DNA segment of 200 bp is amplified to evaluate the PCR performance under the various operational parameters. The initial concentration for accomplishing the PCR process is at least 20 ng/μL at the flow rate of 0.4 μL/min in the portable continuous flow PCR (CFPCR) device. To our knowledge, our group is the first to introduce Arduino boards into the heat control and sample pumping modules for a CFPCR device. MDPI 2022-08-11 /pmc/articles/PMC9412515/ /pubmed/36014212 http://dx.doi.org/10.3390/mi13081289 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sheu, Shyang-Chwen
Song, Yi-Syuan
Chen, Jyh-Jian
A Portable Continuous-Flow Polymerase Chain Reaction Chip Device Integrated with Arduino Boards for Detecting Colla corii asini
title A Portable Continuous-Flow Polymerase Chain Reaction Chip Device Integrated with Arduino Boards for Detecting Colla corii asini
title_full A Portable Continuous-Flow Polymerase Chain Reaction Chip Device Integrated with Arduino Boards for Detecting Colla corii asini
title_fullStr A Portable Continuous-Flow Polymerase Chain Reaction Chip Device Integrated with Arduino Boards for Detecting Colla corii asini
title_full_unstemmed A Portable Continuous-Flow Polymerase Chain Reaction Chip Device Integrated with Arduino Boards for Detecting Colla corii asini
title_short A Portable Continuous-Flow Polymerase Chain Reaction Chip Device Integrated with Arduino Boards for Detecting Colla corii asini
title_sort portable continuous-flow polymerase chain reaction chip device integrated with arduino boards for detecting colla corii asini
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9412515/
https://www.ncbi.nlm.nih.gov/pubmed/36014212
http://dx.doi.org/10.3390/mi13081289
work_keys_str_mv AT sheushyangchwen aportablecontinuousflowpolymerasechainreactionchipdeviceintegratedwitharduinoboardsfordetectingcollacoriiasini
AT songyisyuan aportablecontinuousflowpolymerasechainreactionchipdeviceintegratedwitharduinoboardsfordetectingcollacoriiasini
AT chenjyhjian aportablecontinuousflowpolymerasechainreactionchipdeviceintegratedwitharduinoboardsfordetectingcollacoriiasini
AT sheushyangchwen portablecontinuousflowpolymerasechainreactionchipdeviceintegratedwitharduinoboardsfordetectingcollacoriiasini
AT songyisyuan portablecontinuousflowpolymerasechainreactionchipdeviceintegratedwitharduinoboardsfordetectingcollacoriiasini
AT chenjyhjian portablecontinuousflowpolymerasechainreactionchipdeviceintegratedwitharduinoboardsfordetectingcollacoriiasini