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Anti-Proliferative Potential of Quercetin Loaded Polymeric Mixed Micelles on Rat C6 and Human U87MG Glioma Cells

Quercetin (Qu) is a natural flavonoid present in many commonly consumed food items and is also identified as a potential anticancer agent. The present study evaluates the Qu-loaded polymeric mixed micelles (Qu-PMMs) against C6 and U87MG glioma cell lines. The Box–Behnken Design (BBD) was employed to...

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Autores principales: Paranthaman, Sathishbabu, Uthaiah, Chinnappa A., Osmani, Riyaz Ali M., Hani, Umme, Ghazwani, Mohammed, Alamri, Ali H., Fatease, Adel Al, Madhunapantula, SubbaRao V., Gowda, Devegowda Vishkante
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9412540/
https://www.ncbi.nlm.nih.gov/pubmed/36015268
http://dx.doi.org/10.3390/pharmaceutics14081643
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author Paranthaman, Sathishbabu
Uthaiah, Chinnappa A.
Osmani, Riyaz Ali M.
Hani, Umme
Ghazwani, Mohammed
Alamri, Ali H.
Fatease, Adel Al
Madhunapantula, SubbaRao V.
Gowda, Devegowda Vishkante
author_facet Paranthaman, Sathishbabu
Uthaiah, Chinnappa A.
Osmani, Riyaz Ali M.
Hani, Umme
Ghazwani, Mohammed
Alamri, Ali H.
Fatease, Adel Al
Madhunapantula, SubbaRao V.
Gowda, Devegowda Vishkante
author_sort Paranthaman, Sathishbabu
collection PubMed
description Quercetin (Qu) is a natural flavonoid present in many commonly consumed food items and is also identified as a potential anticancer agent. The present study evaluates the Qu-loaded polymeric mixed micelles (Qu-PMMs) against C6 and U87MG glioma cell lines. The Box–Behnken Design (BBD) was employed to study the influence of independent variables such as Soluplus, Vitamin-E polyethyleneglycol-1000 succinate (E-TPGS), and poloxamer 407 concentrations on dependent variables including particle size (PS), polydispersity index (PDI), and percentage entrapment efficiency (%EE) of the prepared Qu-PMMs. The Qu-PMMs were further characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Scanning Electron Microscope (SEM), and were assessed for in vitro drug release, effect on cell viability, migration, cellular uptake, and apoptosis assays. The PS, PDI, and % EE of the optimized PMMs were 107.16 ± 1.06 nm, 0.236 ± 0.053, and 77.46 ± 1.94%, respectively. The FTIR and XRD revealed that the Qu was completely entrapped inside the PMMs. The SEM analysis confirmed the spherical shape of micelles. The in vitro cell viability study showed that the Qu-PMMs had 1.7 times higher cytotoxicity against C6 and U87MG cells than Qu pure drug (Qu-PD). Furthermore, Qu-PMMs demonstrated superior cellular uptake, inhibited migration, and induced apoptosis when tested against C6 and U87MG cells than pure Qu. Thus, the polymeric mixed micelle (PMMs) enhanced the therapeutic effect of Qu and can be considered an effective therapeutic strategy to treat Glioma.
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spelling pubmed-94125402022-08-27 Anti-Proliferative Potential of Quercetin Loaded Polymeric Mixed Micelles on Rat C6 and Human U87MG Glioma Cells Paranthaman, Sathishbabu Uthaiah, Chinnappa A. Osmani, Riyaz Ali M. Hani, Umme Ghazwani, Mohammed Alamri, Ali H. Fatease, Adel Al Madhunapantula, SubbaRao V. Gowda, Devegowda Vishkante Pharmaceutics Article Quercetin (Qu) is a natural flavonoid present in many commonly consumed food items and is also identified as a potential anticancer agent. The present study evaluates the Qu-loaded polymeric mixed micelles (Qu-PMMs) against C6 and U87MG glioma cell lines. The Box–Behnken Design (BBD) was employed to study the influence of independent variables such as Soluplus, Vitamin-E polyethyleneglycol-1000 succinate (E-TPGS), and poloxamer 407 concentrations on dependent variables including particle size (PS), polydispersity index (PDI), and percentage entrapment efficiency (%EE) of the prepared Qu-PMMs. The Qu-PMMs were further characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Scanning Electron Microscope (SEM), and were assessed for in vitro drug release, effect on cell viability, migration, cellular uptake, and apoptosis assays. The PS, PDI, and % EE of the optimized PMMs were 107.16 ± 1.06 nm, 0.236 ± 0.053, and 77.46 ± 1.94%, respectively. The FTIR and XRD revealed that the Qu was completely entrapped inside the PMMs. The SEM analysis confirmed the spherical shape of micelles. The in vitro cell viability study showed that the Qu-PMMs had 1.7 times higher cytotoxicity against C6 and U87MG cells than Qu pure drug (Qu-PD). Furthermore, Qu-PMMs demonstrated superior cellular uptake, inhibited migration, and induced apoptosis when tested against C6 and U87MG cells than pure Qu. Thus, the polymeric mixed micelle (PMMs) enhanced the therapeutic effect of Qu and can be considered an effective therapeutic strategy to treat Glioma. MDPI 2022-08-06 /pmc/articles/PMC9412540/ /pubmed/36015268 http://dx.doi.org/10.3390/pharmaceutics14081643 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Paranthaman, Sathishbabu
Uthaiah, Chinnappa A.
Osmani, Riyaz Ali M.
Hani, Umme
Ghazwani, Mohammed
Alamri, Ali H.
Fatease, Adel Al
Madhunapantula, SubbaRao V.
Gowda, Devegowda Vishkante
Anti-Proliferative Potential of Quercetin Loaded Polymeric Mixed Micelles on Rat C6 and Human U87MG Glioma Cells
title Anti-Proliferative Potential of Quercetin Loaded Polymeric Mixed Micelles on Rat C6 and Human U87MG Glioma Cells
title_full Anti-Proliferative Potential of Quercetin Loaded Polymeric Mixed Micelles on Rat C6 and Human U87MG Glioma Cells
title_fullStr Anti-Proliferative Potential of Quercetin Loaded Polymeric Mixed Micelles on Rat C6 and Human U87MG Glioma Cells
title_full_unstemmed Anti-Proliferative Potential of Quercetin Loaded Polymeric Mixed Micelles on Rat C6 and Human U87MG Glioma Cells
title_short Anti-Proliferative Potential of Quercetin Loaded Polymeric Mixed Micelles on Rat C6 and Human U87MG Glioma Cells
title_sort anti-proliferative potential of quercetin loaded polymeric mixed micelles on rat c6 and human u87mg glioma cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9412540/
https://www.ncbi.nlm.nih.gov/pubmed/36015268
http://dx.doi.org/10.3390/pharmaceutics14081643
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