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Developing a Sensitive Platform to Measure 5-Methyltetrahydrofolate in Subjects with MTHFR and PON1 Gene Polymorphisms

Inadequate levels of 5-methyltetrahydrofolate (5-MTHF) and the T variant of MTHFR C677T have been suggested to be associated with an increased risk of developing mental illness, whereas the PON1 SNP variant provides a protective role. However, reports validating the methodology for plasma 5-MTHF lev...

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Autores principales: Lu, Mong-Liang, Ku, Wei-Chi, Syifa, Nailis, Hu, Shu-Chin, Chou, Chia-Te, Wu, Yi-Hsio, Kuo, Po-Hsiu, Chen, Chun-Hsin, Chen, Wei J., Wu, Tzu-Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9412631/
https://www.ncbi.nlm.nih.gov/pubmed/36014826
http://dx.doi.org/10.3390/nu14163320
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author Lu, Mong-Liang
Ku, Wei-Chi
Syifa, Nailis
Hu, Shu-Chin
Chou, Chia-Te
Wu, Yi-Hsio
Kuo, Po-Hsiu
Chen, Chun-Hsin
Chen, Wei J.
Wu, Tzu-Hua
author_facet Lu, Mong-Liang
Ku, Wei-Chi
Syifa, Nailis
Hu, Shu-Chin
Chou, Chia-Te
Wu, Yi-Hsio
Kuo, Po-Hsiu
Chen, Chun-Hsin
Chen, Wei J.
Wu, Tzu-Hua
author_sort Lu, Mong-Liang
collection PubMed
description Inadequate levels of 5-methyltetrahydrofolate (5-MTHF) and the T variant of MTHFR C677T have been suggested to be associated with an increased risk of developing mental illness, whereas the PON1 SNP variant provides a protective role. However, reports validating the methodology for plasma 5-MTHF levels in schizophrenia patients are limited. A sensitive LC–MS/MS system using an amide column and calibration curve was determined by dialyzed human plasma, and applied to schizophrenia patients and healthy controls in Taiwan, and the differences between the subgroups were discussed. This analysis system meets regulation criteria, and the lower limit of quantification for 5-MTHF levels was 4 nM from 200 μL plasma, within 7 min. The mean plasma 5-MTHF levels in schizophrenia patients (n = 34; 11.70 ± 10.37 nM) were lower than those in the healthy controls (n = 42; 22.67 ± 11.12 nM) significantly (p < 0.01). 5-MTHF concentrations were significantly lower in male carriers than in female carriers (18.30 ± 10.37 nM vs. 24.83 ± 11.01 nM, p < 0.05), especially in subjects who were MTHFR CT/PON1 Q allele carriers. In conclusion, this quantitative system, which employed sensitive and simple processing methods, was successfully applied, and identified that schizophrenic patients had significantly lower levels of 5-MTHF. Lower plasma 5-MTHF concentrations were observed in male subjects.
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spelling pubmed-94126312022-08-27 Developing a Sensitive Platform to Measure 5-Methyltetrahydrofolate in Subjects with MTHFR and PON1 Gene Polymorphisms Lu, Mong-Liang Ku, Wei-Chi Syifa, Nailis Hu, Shu-Chin Chou, Chia-Te Wu, Yi-Hsio Kuo, Po-Hsiu Chen, Chun-Hsin Chen, Wei J. Wu, Tzu-Hua Nutrients Article Inadequate levels of 5-methyltetrahydrofolate (5-MTHF) and the T variant of MTHFR C677T have been suggested to be associated with an increased risk of developing mental illness, whereas the PON1 SNP variant provides a protective role. However, reports validating the methodology for plasma 5-MTHF levels in schizophrenia patients are limited. A sensitive LC–MS/MS system using an amide column and calibration curve was determined by dialyzed human plasma, and applied to schizophrenia patients and healthy controls in Taiwan, and the differences between the subgroups were discussed. This analysis system meets regulation criteria, and the lower limit of quantification for 5-MTHF levels was 4 nM from 200 μL plasma, within 7 min. The mean plasma 5-MTHF levels in schizophrenia patients (n = 34; 11.70 ± 10.37 nM) were lower than those in the healthy controls (n = 42; 22.67 ± 11.12 nM) significantly (p < 0.01). 5-MTHF concentrations were significantly lower in male carriers than in female carriers (18.30 ± 10.37 nM vs. 24.83 ± 11.01 nM, p < 0.05), especially in subjects who were MTHFR CT/PON1 Q allele carriers. In conclusion, this quantitative system, which employed sensitive and simple processing methods, was successfully applied, and identified that schizophrenic patients had significantly lower levels of 5-MTHF. Lower plasma 5-MTHF concentrations were observed in male subjects. MDPI 2022-08-13 /pmc/articles/PMC9412631/ /pubmed/36014826 http://dx.doi.org/10.3390/nu14163320 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lu, Mong-Liang
Ku, Wei-Chi
Syifa, Nailis
Hu, Shu-Chin
Chou, Chia-Te
Wu, Yi-Hsio
Kuo, Po-Hsiu
Chen, Chun-Hsin
Chen, Wei J.
Wu, Tzu-Hua
Developing a Sensitive Platform to Measure 5-Methyltetrahydrofolate in Subjects with MTHFR and PON1 Gene Polymorphisms
title Developing a Sensitive Platform to Measure 5-Methyltetrahydrofolate in Subjects with MTHFR and PON1 Gene Polymorphisms
title_full Developing a Sensitive Platform to Measure 5-Methyltetrahydrofolate in Subjects with MTHFR and PON1 Gene Polymorphisms
title_fullStr Developing a Sensitive Platform to Measure 5-Methyltetrahydrofolate in Subjects with MTHFR and PON1 Gene Polymorphisms
title_full_unstemmed Developing a Sensitive Platform to Measure 5-Methyltetrahydrofolate in Subjects with MTHFR and PON1 Gene Polymorphisms
title_short Developing a Sensitive Platform to Measure 5-Methyltetrahydrofolate in Subjects with MTHFR and PON1 Gene Polymorphisms
title_sort developing a sensitive platform to measure 5-methyltetrahydrofolate in subjects with mthfr and pon1 gene polymorphisms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9412631/
https://www.ncbi.nlm.nih.gov/pubmed/36014826
http://dx.doi.org/10.3390/nu14163320
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