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Improved systemic half-life of glucagon-like peptide-1-loaded carbonate apatite nanoparticles in rats
BACKGROUND: Glucagon-like peptide-1 (GLP1) is an endogenous peptide that regulates blood glucose level. But its susceptibility to rapid metabolic degradation limits its therapeutic use. AIM: To prepare GLP1-encapsulated nanosize particle with controlled release property to improve the systemic half-...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Baishideng Publishing Group Inc
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9412859/ https://www.ncbi.nlm.nih.gov/pubmed/36159222 http://dx.doi.org/10.4239/wjd.v13.i8.613 |
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author | Ibnat, Nabilah Zaman, Rahela Uddin, Mohammad Borhan Chowdhury, Ezharul Lee, Chooi Yeng |
author_facet | Ibnat, Nabilah Zaman, Rahela Uddin, Mohammad Borhan Chowdhury, Ezharul Lee, Chooi Yeng |
author_sort | Ibnat, Nabilah |
collection | PubMed |
description | BACKGROUND: Glucagon-like peptide-1 (GLP1) is an endogenous peptide that regulates blood glucose level. But its susceptibility to rapid metabolic degradation limits its therapeutic use. AIM: To prepare GLP1-encapsulated nanosize particle with controlled release property to improve the systemic half-life of GLP1. METHODS: GLP1 nanoparticles were prepared by complexation of GLP1 with carbonate apatite nanoparticles (CA NPs). The physicochemical properties of the CA NPs, the effects of GLP1-loaded CA NPs on cell viability, and the systemic bioavailability of GLP1 after CA NPs administration were determined. RESULTS: The GLP1-loaded CA NPs was within 200 nm in size and stable in fetal bovine serum. The formulation did not affect the viability of human cell lines suggesting that the accumulation of CA NPs in target tissues is safe. In Sprague Dawley rats, the plasma GLP1 Levels as measured from the GLP1-loaded CA NPs-treated rats, were significantly higher than that of the control rats and free GLP1-treated rats at 1 h post-treatment (P < 0.05), and the level remained higher than the other two groups for at least 4 h. CONCLUSION: The GLP1-loaded CA NPs improved the plasma half-life of GLP1. The systemic bioavailability of GLP1 is longer than other GLP1 nanoparticles reported to date. |
format | Online Article Text |
id | pubmed-9412859 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Baishideng Publishing Group Inc |
record_format | MEDLINE/PubMed |
spelling | pubmed-94128592022-09-23 Improved systemic half-life of glucagon-like peptide-1-loaded carbonate apatite nanoparticles in rats Ibnat, Nabilah Zaman, Rahela Uddin, Mohammad Borhan Chowdhury, Ezharul Lee, Chooi Yeng World J Diabetes Basic Study BACKGROUND: Glucagon-like peptide-1 (GLP1) is an endogenous peptide that regulates blood glucose level. But its susceptibility to rapid metabolic degradation limits its therapeutic use. AIM: To prepare GLP1-encapsulated nanosize particle with controlled release property to improve the systemic half-life of GLP1. METHODS: GLP1 nanoparticles were prepared by complexation of GLP1 with carbonate apatite nanoparticles (CA NPs). The physicochemical properties of the CA NPs, the effects of GLP1-loaded CA NPs on cell viability, and the systemic bioavailability of GLP1 after CA NPs administration were determined. RESULTS: The GLP1-loaded CA NPs was within 200 nm in size and stable in fetal bovine serum. The formulation did not affect the viability of human cell lines suggesting that the accumulation of CA NPs in target tissues is safe. In Sprague Dawley rats, the plasma GLP1 Levels as measured from the GLP1-loaded CA NPs-treated rats, were significantly higher than that of the control rats and free GLP1-treated rats at 1 h post-treatment (P < 0.05), and the level remained higher than the other two groups for at least 4 h. CONCLUSION: The GLP1-loaded CA NPs improved the plasma half-life of GLP1. The systemic bioavailability of GLP1 is longer than other GLP1 nanoparticles reported to date. Baishideng Publishing Group Inc 2022-08-15 2022-08-15 /pmc/articles/PMC9412859/ /pubmed/36159222 http://dx.doi.org/10.4239/wjd.v13.i8.613 Text en ©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved. https://creativecommons.org/licenses/by-nc/4.0/This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. |
spellingShingle | Basic Study Ibnat, Nabilah Zaman, Rahela Uddin, Mohammad Borhan Chowdhury, Ezharul Lee, Chooi Yeng Improved systemic half-life of glucagon-like peptide-1-loaded carbonate apatite nanoparticles in rats |
title | Improved systemic half-life of glucagon-like peptide-1-loaded carbonate apatite nanoparticles in rats |
title_full | Improved systemic half-life of glucagon-like peptide-1-loaded carbonate apatite nanoparticles in rats |
title_fullStr | Improved systemic half-life of glucagon-like peptide-1-loaded carbonate apatite nanoparticles in rats |
title_full_unstemmed | Improved systemic half-life of glucagon-like peptide-1-loaded carbonate apatite nanoparticles in rats |
title_short | Improved systemic half-life of glucagon-like peptide-1-loaded carbonate apatite nanoparticles in rats |
title_sort | improved systemic half-life of glucagon-like peptide-1-loaded carbonate apatite nanoparticles in rats |
topic | Basic Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9412859/ https://www.ncbi.nlm.nih.gov/pubmed/36159222 http://dx.doi.org/10.4239/wjd.v13.i8.613 |
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