Cargando…
Serological Investigation and Genetic Characteristics of Pseudorabies Virus between 2019 and 2021 in Henan Province of China
In late 2011, severe pseudorabies (PR) outbreaks occurred among swine herds vaccinated with the Bartha-K61 vaccine in many provinces of China, causing enormous economic losses for the pork industry. To understand the epidemic profile and genetic characteristics of the pseudorabies virus (PRV), a tot...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9412869/ https://www.ncbi.nlm.nih.gov/pubmed/36016307 http://dx.doi.org/10.3390/v14081685 |
_version_ | 1784775598605860864 |
---|---|
author | Chen, Ximeng Li, Hongxuan Zhu, Qianlei Chen, Hongying Wang, Zhenya Zheng, Lanlan Liu, Fang Wei, Zhanyong |
author_facet | Chen, Ximeng Li, Hongxuan Zhu, Qianlei Chen, Hongying Wang, Zhenya Zheng, Lanlan Liu, Fang Wei, Zhanyong |
author_sort | Chen, Ximeng |
collection | PubMed |
description | In late 2011, severe pseudorabies (PR) outbreaks occurred among swine herds vaccinated with the Bartha-K61 vaccine in many provinces of China, causing enormous economic losses for the pork industry. To understand the epidemic profile and genetic characteristics of the pseudorabies virus (PRV), a total of 35,796 serum samples were collected from 1090 pig farms of different breeding scales between 2019 and 2021 in the Henan province where swine had been immunized with the Bartha-K61 vaccine, and PRV glycoprotein E (gE)-specific antibodies were detected using an enzyme-linked immunosorbent assay (ELISA). The results reveal that the overall positive rate for PRV gE antibodies was 20.33% (7276/35,796), which decreased from 25.00% (2596/10,385) in 2019 to 16.69% (2222/13,315) in 2021, demonstrating that PR still existed widely in pig herds in the Henan province but displayed a decreasing trend. Further analysis suggested that the PRV-seropositive rate may be associated with farm size, farm category, quarter, region and the cross-regional transportation of livestock. Moreover, the gE gene complete sequences of 18 PRV isolates were obtained, and they shared a high identity (97.1–100.0%) with reference strains at the nucleotide level. Interestingly, the phylogenetic analysis based on the gE complete sequences found that there were both classical strains and variant strains in pig herds. The deduced amino acid sequence analysis of the gE gene showed that there were unique amino acids in the classical strains, the variant strains and genotype Ⅱ strains. This study provides epidemiological data that could be useful in the prevention of pseudorabies in Henan, China, and this finding contributed to our understanding of the epidemiology and evolution of PRV. |
format | Online Article Text |
id | pubmed-9412869 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94128692022-08-27 Serological Investigation and Genetic Characteristics of Pseudorabies Virus between 2019 and 2021 in Henan Province of China Chen, Ximeng Li, Hongxuan Zhu, Qianlei Chen, Hongying Wang, Zhenya Zheng, Lanlan Liu, Fang Wei, Zhanyong Viruses Article In late 2011, severe pseudorabies (PR) outbreaks occurred among swine herds vaccinated with the Bartha-K61 vaccine in many provinces of China, causing enormous economic losses for the pork industry. To understand the epidemic profile and genetic characteristics of the pseudorabies virus (PRV), a total of 35,796 serum samples were collected from 1090 pig farms of different breeding scales between 2019 and 2021 in the Henan province where swine had been immunized with the Bartha-K61 vaccine, and PRV glycoprotein E (gE)-specific antibodies were detected using an enzyme-linked immunosorbent assay (ELISA). The results reveal that the overall positive rate for PRV gE antibodies was 20.33% (7276/35,796), which decreased from 25.00% (2596/10,385) in 2019 to 16.69% (2222/13,315) in 2021, demonstrating that PR still existed widely in pig herds in the Henan province but displayed a decreasing trend. Further analysis suggested that the PRV-seropositive rate may be associated with farm size, farm category, quarter, region and the cross-regional transportation of livestock. Moreover, the gE gene complete sequences of 18 PRV isolates were obtained, and they shared a high identity (97.1–100.0%) with reference strains at the nucleotide level. Interestingly, the phylogenetic analysis based on the gE complete sequences found that there were both classical strains and variant strains in pig herds. The deduced amino acid sequence analysis of the gE gene showed that there were unique amino acids in the classical strains, the variant strains and genotype Ⅱ strains. This study provides epidemiological data that could be useful in the prevention of pseudorabies in Henan, China, and this finding contributed to our understanding of the epidemiology and evolution of PRV. MDPI 2022-07-30 /pmc/articles/PMC9412869/ /pubmed/36016307 http://dx.doi.org/10.3390/v14081685 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chen, Ximeng Li, Hongxuan Zhu, Qianlei Chen, Hongying Wang, Zhenya Zheng, Lanlan Liu, Fang Wei, Zhanyong Serological Investigation and Genetic Characteristics of Pseudorabies Virus between 2019 and 2021 in Henan Province of China |
title | Serological Investigation and Genetic Characteristics of Pseudorabies Virus between 2019 and 2021 in Henan Province of China |
title_full | Serological Investigation and Genetic Characteristics of Pseudorabies Virus between 2019 and 2021 in Henan Province of China |
title_fullStr | Serological Investigation and Genetic Characteristics of Pseudorabies Virus between 2019 and 2021 in Henan Province of China |
title_full_unstemmed | Serological Investigation and Genetic Characteristics of Pseudorabies Virus between 2019 and 2021 in Henan Province of China |
title_short | Serological Investigation and Genetic Characteristics of Pseudorabies Virus between 2019 and 2021 in Henan Province of China |
title_sort | serological investigation and genetic characteristics of pseudorabies virus between 2019 and 2021 in henan province of china |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9412869/ https://www.ncbi.nlm.nih.gov/pubmed/36016307 http://dx.doi.org/10.3390/v14081685 |
work_keys_str_mv | AT chenximeng serologicalinvestigationandgeneticcharacteristicsofpseudorabiesvirusbetween2019and2021inhenanprovinceofchina AT lihongxuan serologicalinvestigationandgeneticcharacteristicsofpseudorabiesvirusbetween2019and2021inhenanprovinceofchina AT zhuqianlei serologicalinvestigationandgeneticcharacteristicsofpseudorabiesvirusbetween2019and2021inhenanprovinceofchina AT chenhongying serologicalinvestigationandgeneticcharacteristicsofpseudorabiesvirusbetween2019and2021inhenanprovinceofchina AT wangzhenya serologicalinvestigationandgeneticcharacteristicsofpseudorabiesvirusbetween2019and2021inhenanprovinceofchina AT zhenglanlan serologicalinvestigationandgeneticcharacteristicsofpseudorabiesvirusbetween2019and2021inhenanprovinceofchina AT liufang serologicalinvestigationandgeneticcharacteristicsofpseudorabiesvirusbetween2019and2021inhenanprovinceofchina AT weizhanyong serologicalinvestigationandgeneticcharacteristicsofpseudorabiesvirusbetween2019and2021inhenanprovinceofchina |