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Isolation and Characterization of a Newly Discovered Phage, V-YDF132, for Lysing Vibrio harveyi
A newly discovered lytic bacteriophage, V-YDF132, which efficiently infects the pathogenic strain of Vibrio harveyi, was isolated from aquaculture water collected in Yangjiang, China. Electron microscopy studies revealed that V-YDF132 belonged to the Siphoviridae family, with an icosahedral head and...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9413028/ https://www.ncbi.nlm.nih.gov/pubmed/36016424 http://dx.doi.org/10.3390/v14081802 |
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author | Kang, Shaozhu Zhang, Luhao Liao, Jiaming Zhang, Dongzhuo Wu, Siting Zhang, Xin Qin, Qiwei Wei, Jingguang |
author_facet | Kang, Shaozhu Zhang, Luhao Liao, Jiaming Zhang, Dongzhuo Wu, Siting Zhang, Xin Qin, Qiwei Wei, Jingguang |
author_sort | Kang, Shaozhu |
collection | PubMed |
description | A newly discovered lytic bacteriophage, V-YDF132, which efficiently infects the pathogenic strain of Vibrio harveyi, was isolated from aquaculture water collected in Yangjiang, China. Electron microscopy studies revealed that V-YDF132 belonged to the Siphoviridae family, with an icosahedral head and a long noncontractile tail. The phage has a latent period of 25 min and a burst size of 298 pfu/infected bacterium. V-YDF132 was stable from 37 to 50 °C. It has a wide range of stability (pH 5–11) and can resist adverse external environments. In addition, in vitro the phage V-YDF132 has a strong lytic effect on the host. Genome sequencing results revealed that V-YDF132 has a DNA genome of 84,375 bp with a GC content of 46.97%. In total, 115 putative open reading frames (ORFs) were predicted in the phage V-YDF132 genome. Meanwhile, the phage genome does not contain any known bacterial virulence genes or antimicrobial resistance genes. Comparison of the genomic features of the phage V-YDF132 and phylogenetic analysis revealed that V-YDF132 is a newly discovered Vibrio phage. Multiple genome comparisons and comparative genomics showed that V-YDF132 is in the same genus as Vibrio phages vB_VpS_PG28 (MT735630.2) and VH2_2019 (MN794238.1). Overall, the results indicate that V-YDF132 is potentially applicable for biological control of vibriosis. |
format | Online Article Text |
id | pubmed-9413028 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94130282022-08-27 Isolation and Characterization of a Newly Discovered Phage, V-YDF132, for Lysing Vibrio harveyi Kang, Shaozhu Zhang, Luhao Liao, Jiaming Zhang, Dongzhuo Wu, Siting Zhang, Xin Qin, Qiwei Wei, Jingguang Viruses Article A newly discovered lytic bacteriophage, V-YDF132, which efficiently infects the pathogenic strain of Vibrio harveyi, was isolated from aquaculture water collected in Yangjiang, China. Electron microscopy studies revealed that V-YDF132 belonged to the Siphoviridae family, with an icosahedral head and a long noncontractile tail. The phage has a latent period of 25 min and a burst size of 298 pfu/infected bacterium. V-YDF132 was stable from 37 to 50 °C. It has a wide range of stability (pH 5–11) and can resist adverse external environments. In addition, in vitro the phage V-YDF132 has a strong lytic effect on the host. Genome sequencing results revealed that V-YDF132 has a DNA genome of 84,375 bp with a GC content of 46.97%. In total, 115 putative open reading frames (ORFs) were predicted in the phage V-YDF132 genome. Meanwhile, the phage genome does not contain any known bacterial virulence genes or antimicrobial resistance genes. Comparison of the genomic features of the phage V-YDF132 and phylogenetic analysis revealed that V-YDF132 is a newly discovered Vibrio phage. Multiple genome comparisons and comparative genomics showed that V-YDF132 is in the same genus as Vibrio phages vB_VpS_PG28 (MT735630.2) and VH2_2019 (MN794238.1). Overall, the results indicate that V-YDF132 is potentially applicable for biological control of vibriosis. MDPI 2022-08-17 /pmc/articles/PMC9413028/ /pubmed/36016424 http://dx.doi.org/10.3390/v14081802 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kang, Shaozhu Zhang, Luhao Liao, Jiaming Zhang, Dongzhuo Wu, Siting Zhang, Xin Qin, Qiwei Wei, Jingguang Isolation and Characterization of a Newly Discovered Phage, V-YDF132, for Lysing Vibrio harveyi |
title | Isolation and Characterization of a Newly Discovered Phage, V-YDF132, for Lysing Vibrio harveyi |
title_full | Isolation and Characterization of a Newly Discovered Phage, V-YDF132, for Lysing Vibrio harveyi |
title_fullStr | Isolation and Characterization of a Newly Discovered Phage, V-YDF132, for Lysing Vibrio harveyi |
title_full_unstemmed | Isolation and Characterization of a Newly Discovered Phage, V-YDF132, for Lysing Vibrio harveyi |
title_short | Isolation and Characterization of a Newly Discovered Phage, V-YDF132, for Lysing Vibrio harveyi |
title_sort | isolation and characterization of a newly discovered phage, v-ydf132, for lysing vibrio harveyi |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9413028/ https://www.ncbi.nlm.nih.gov/pubmed/36016424 http://dx.doi.org/10.3390/v14081802 |
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