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Rapid Limit Test of Seven Pesticide Residues in Tea Based on the Combination of TLC and Raman Imaging Microscopy

Numerous studies have shown that pesticide residues in tea exceeding the maximum residue limits (MRL) can cause harmful effects on the human body. There are many limitations in the existing analytical methods for pesticide residues in tea, so new analytical methods need to be developed. We developed...

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Autores principales: Liang, Xin, Li, Li, Han, Cuiyan, Dong, Yan, Xu, Feng, Lv, Zhen, Zhang, Ying, Qu, Zhijie, Dong, Wei, Sun, Yongqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9413101/
https://www.ncbi.nlm.nih.gov/pubmed/36014399
http://dx.doi.org/10.3390/molecules27165151
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author Liang, Xin
Li, Li
Han, Cuiyan
Dong, Yan
Xu, Feng
Lv, Zhen
Zhang, Ying
Qu, Zhijie
Dong, Wei
Sun, Yongqiang
author_facet Liang, Xin
Li, Li
Han, Cuiyan
Dong, Yan
Xu, Feng
Lv, Zhen
Zhang, Ying
Qu, Zhijie
Dong, Wei
Sun, Yongqiang
author_sort Liang, Xin
collection PubMed
description Numerous studies have shown that pesticide residues in tea exceeding the maximum residue limits (MRL) can cause harmful effects on the human body. There are many limitations in the existing analytical methods for pesticide residues in tea, so new analytical methods need to be developed. We developed a limit test method that combines thin-layer chromatography with Raman imaging microscopy (TLC-RIM). Seven residual pesticide components in tea (Avermectin, Methomyl, Carbendazim, Imidacloprid, Chlorothalonil, Azoxystrobin, and Acetamiprid) could be preliminarily separated by TLC and then irradiated by a 532 nm laser. Raman spectra of seven pesticides obtained by Raman imaging microscopy could be used to test whether the pesticide residues in tea exceed the MRL. The limits of detection of the seven pesticides were 0.04, 0.10, 0.24, 0.20, 0.12, 0.12, and 1.0 mg/mL, respectively. The simulated positive test showed that the matrix in tea did not interfere with the test of the seven pesticides. When the pesticides were tested within 8 h, the RSD of the peak heights of the seven pesticides were 1.2%~9.6%; the test results of three batches of tea showed that the imidacloprid in one batch of tea exceeded its MRL, and the results were consistent with that by UPLC-MS/MS. The TLC-RIM is fast, sensitive, stable, specific, and reliable.
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spelling pubmed-94131012022-08-27 Rapid Limit Test of Seven Pesticide Residues in Tea Based on the Combination of TLC and Raman Imaging Microscopy Liang, Xin Li, Li Han, Cuiyan Dong, Yan Xu, Feng Lv, Zhen Zhang, Ying Qu, Zhijie Dong, Wei Sun, Yongqiang Molecules Article Numerous studies have shown that pesticide residues in tea exceeding the maximum residue limits (MRL) can cause harmful effects on the human body. There are many limitations in the existing analytical methods for pesticide residues in tea, so new analytical methods need to be developed. We developed a limit test method that combines thin-layer chromatography with Raman imaging microscopy (TLC-RIM). Seven residual pesticide components in tea (Avermectin, Methomyl, Carbendazim, Imidacloprid, Chlorothalonil, Azoxystrobin, and Acetamiprid) could be preliminarily separated by TLC and then irradiated by a 532 nm laser. Raman spectra of seven pesticides obtained by Raman imaging microscopy could be used to test whether the pesticide residues in tea exceed the MRL. The limits of detection of the seven pesticides were 0.04, 0.10, 0.24, 0.20, 0.12, 0.12, and 1.0 mg/mL, respectively. The simulated positive test showed that the matrix in tea did not interfere with the test of the seven pesticides. When the pesticides were tested within 8 h, the RSD of the peak heights of the seven pesticides were 1.2%~9.6%; the test results of three batches of tea showed that the imidacloprid in one batch of tea exceeded its MRL, and the results were consistent with that by UPLC-MS/MS. The TLC-RIM is fast, sensitive, stable, specific, and reliable. MDPI 2022-08-12 /pmc/articles/PMC9413101/ /pubmed/36014399 http://dx.doi.org/10.3390/molecules27165151 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liang, Xin
Li, Li
Han, Cuiyan
Dong, Yan
Xu, Feng
Lv, Zhen
Zhang, Ying
Qu, Zhijie
Dong, Wei
Sun, Yongqiang
Rapid Limit Test of Seven Pesticide Residues in Tea Based on the Combination of TLC and Raman Imaging Microscopy
title Rapid Limit Test of Seven Pesticide Residues in Tea Based on the Combination of TLC and Raman Imaging Microscopy
title_full Rapid Limit Test of Seven Pesticide Residues in Tea Based on the Combination of TLC and Raman Imaging Microscopy
title_fullStr Rapid Limit Test of Seven Pesticide Residues in Tea Based on the Combination of TLC and Raman Imaging Microscopy
title_full_unstemmed Rapid Limit Test of Seven Pesticide Residues in Tea Based on the Combination of TLC and Raman Imaging Microscopy
title_short Rapid Limit Test of Seven Pesticide Residues in Tea Based on the Combination of TLC and Raman Imaging Microscopy
title_sort rapid limit test of seven pesticide residues in tea based on the combination of tlc and raman imaging microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9413101/
https://www.ncbi.nlm.nih.gov/pubmed/36014399
http://dx.doi.org/10.3390/molecules27165151
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