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Insight into the molecular mechanisms of leaf coloration in Cymbidium ensifolium
Cymbidium ensifolium L. is a significant ornamental plant in Orchidaceae. Aside from its attractive flowers, its leaf coloration is also an important ornamental trait. However, there is an apparent lack of studies concerning the intricate mechanism of leaf coloration in C. ensifolium. In this study,...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9413228/ https://www.ncbi.nlm.nih.gov/pubmed/36035180 http://dx.doi.org/10.3389/fgene.2022.923082 |
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author | Cao, Hua Li, Han Chen, Xiang Zhang, Yuying Lu, Lin Li, Shenchong Tao, Xiang Zhu, WeiYin Wang, Jihua Ma, Lulin |
author_facet | Cao, Hua Li, Han Chen, Xiang Zhang, Yuying Lu, Lin Li, Shenchong Tao, Xiang Zhu, WeiYin Wang, Jihua Ma, Lulin |
author_sort | Cao, Hua |
collection | PubMed |
description | Cymbidium ensifolium L. is a significant ornamental plant in Orchidaceae. Aside from its attractive flowers, its leaf coloration is also an important ornamental trait. However, there is an apparent lack of studies concerning the intricate mechanism of leaf coloration in C. ensifolium. In this study, we report a systematic evaluation of leaf coloration utilizing transcriptome and metabolome profiles of purple, yellow, and green leaves. In total, 40 anthocyanins and 67 flavonoids were quantified along with chlorophyll content. The tissue–transcriptome profile identified 26,499 differentially expressed genes (DEGs). The highest chlorophyll contents were identified in green leaves, followed by yellow and purple leaves. We identified key anthocyanins and flavonoids associated with leaf coloration, including cyanidin-3-O-sophoroside, naringenin-7-O-glucoside, delphinidin, cyanidin, petunidin, and quercetin, diosmetin, sinensetin, and naringenin chalcone. Moreover, genes encoding UDP-glucoronosyl, UDP-glucosyl transferase, chalcone synthesis, flavodoxin, cytochrome P450, and AMP-binding enzyme were identified as key structural genes affecting leaf coloration in C. ensifolium. In summary, copigmentation resulting from several key metabolites modulated by structural genes was identified as governing leaf coloration in C. ensifolium. Further functional verification of the identified DEGs and co-accumulation of metabolites can provide a tool to modify leaf color and improve the aesthetic value of C. ensifolium. |
format | Online Article Text |
id | pubmed-9413228 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94132282022-08-27 Insight into the molecular mechanisms of leaf coloration in Cymbidium ensifolium Cao, Hua Li, Han Chen, Xiang Zhang, Yuying Lu, Lin Li, Shenchong Tao, Xiang Zhu, WeiYin Wang, Jihua Ma, Lulin Front Genet Genetics Cymbidium ensifolium L. is a significant ornamental plant in Orchidaceae. Aside from its attractive flowers, its leaf coloration is also an important ornamental trait. However, there is an apparent lack of studies concerning the intricate mechanism of leaf coloration in C. ensifolium. In this study, we report a systematic evaluation of leaf coloration utilizing transcriptome and metabolome profiles of purple, yellow, and green leaves. In total, 40 anthocyanins and 67 flavonoids were quantified along with chlorophyll content. The tissue–transcriptome profile identified 26,499 differentially expressed genes (DEGs). The highest chlorophyll contents were identified in green leaves, followed by yellow and purple leaves. We identified key anthocyanins and flavonoids associated with leaf coloration, including cyanidin-3-O-sophoroside, naringenin-7-O-glucoside, delphinidin, cyanidin, petunidin, and quercetin, diosmetin, sinensetin, and naringenin chalcone. Moreover, genes encoding UDP-glucoronosyl, UDP-glucosyl transferase, chalcone synthesis, flavodoxin, cytochrome P450, and AMP-binding enzyme were identified as key structural genes affecting leaf coloration in C. ensifolium. In summary, copigmentation resulting from several key metabolites modulated by structural genes was identified as governing leaf coloration in C. ensifolium. Further functional verification of the identified DEGs and co-accumulation of metabolites can provide a tool to modify leaf color and improve the aesthetic value of C. ensifolium. Frontiers Media S.A. 2022-08-12 /pmc/articles/PMC9413228/ /pubmed/36035180 http://dx.doi.org/10.3389/fgene.2022.923082 Text en Copyright © 2022 Cao, Li, Chen, Zhang, Lu, Li, Tao, Zhu, Wang and Ma. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Cao, Hua Li, Han Chen, Xiang Zhang, Yuying Lu, Lin Li, Shenchong Tao, Xiang Zhu, WeiYin Wang, Jihua Ma, Lulin Insight into the molecular mechanisms of leaf coloration in Cymbidium ensifolium |
title | Insight into the molecular mechanisms of leaf coloration in Cymbidium ensifolium
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title_full | Insight into the molecular mechanisms of leaf coloration in Cymbidium ensifolium
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title_fullStr | Insight into the molecular mechanisms of leaf coloration in Cymbidium ensifolium
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title_full_unstemmed | Insight into the molecular mechanisms of leaf coloration in Cymbidium ensifolium
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title_short | Insight into the molecular mechanisms of leaf coloration in Cymbidium ensifolium
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title_sort | insight into the molecular mechanisms of leaf coloration in cymbidium ensifolium |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9413228/ https://www.ncbi.nlm.nih.gov/pubmed/36035180 http://dx.doi.org/10.3389/fgene.2022.923082 |
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