Cargando…

Robust Assembly of Cross-Linked Protein Nanofibrils into Hierarchically Structured Microfibers

[Image: see text] Natural, high-performance fibers generally have hierarchically organized nanosized building blocks. Inspired by this, whey protein nanofibrils (PNFs) are assembled into microfibers, using flow-focusing. By adding genipin as a nontoxic cross-linker to the PNF suspension before spinn...

Descripción completa

Detalles Bibliográficos
Autores principales: Ye, Xinchen, Capezza, Antonio J., Davoodi, Saeed, Wei, Xin-Feng, Andersson, Richard L., Chumakov, Andrei, Roth, Stephan V., Langton, Maud, Lundell, Fredrik, Hedenqvist, Mikael S., Lendel, Christofer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9413408/
https://www.ncbi.nlm.nih.gov/pubmed/35904348
http://dx.doi.org/10.1021/acsnano.2c03790
_version_ 1784775734550593536
author Ye, Xinchen
Capezza, Antonio J.
Davoodi, Saeed
Wei, Xin-Feng
Andersson, Richard L.
Chumakov, Andrei
Roth, Stephan V.
Langton, Maud
Lundell, Fredrik
Hedenqvist, Mikael S.
Lendel, Christofer
author_facet Ye, Xinchen
Capezza, Antonio J.
Davoodi, Saeed
Wei, Xin-Feng
Andersson, Richard L.
Chumakov, Andrei
Roth, Stephan V.
Langton, Maud
Lundell, Fredrik
Hedenqvist, Mikael S.
Lendel, Christofer
author_sort Ye, Xinchen
collection PubMed
description [Image: see text] Natural, high-performance fibers generally have hierarchically organized nanosized building blocks. Inspired by this, whey protein nanofibrils (PNFs) are assembled into microfibers, using flow-focusing. By adding genipin as a nontoxic cross-linker to the PNF suspension before spinning, significantly improved mechanical properties of the final fiber are obtained. For curved PNFs, with a low content of cross-linker (2%) the fiber is almost 3 times stronger and 4 times stiffer than the fiber without a cross-linker. At higher content of genipin (10%), the elongation at break increases by a factor of 2 and the energy at break increases by a factor of 5. The cross-linking also enables the spinning of microfibers from long straight PNFs, which has not been achieved before. These microfibers have higher stiffness and strength but lower ductility and toughness than those made from curved PNFs. The fibers spun from the two classes of nanofibrils show clear morphological differences. The study demonstrates the production of protein-based microfibers with mechanical properties similar to natural protein-based fibers and provides insights about the role of the nanostructure in the assembly process.
format Online
Article
Text
id pubmed-9413408
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-94134082022-08-27 Robust Assembly of Cross-Linked Protein Nanofibrils into Hierarchically Structured Microfibers Ye, Xinchen Capezza, Antonio J. Davoodi, Saeed Wei, Xin-Feng Andersson, Richard L. Chumakov, Andrei Roth, Stephan V. Langton, Maud Lundell, Fredrik Hedenqvist, Mikael S. Lendel, Christofer ACS Nano [Image: see text] Natural, high-performance fibers generally have hierarchically organized nanosized building blocks. Inspired by this, whey protein nanofibrils (PNFs) are assembled into microfibers, using flow-focusing. By adding genipin as a nontoxic cross-linker to the PNF suspension before spinning, significantly improved mechanical properties of the final fiber are obtained. For curved PNFs, with a low content of cross-linker (2%) the fiber is almost 3 times stronger and 4 times stiffer than the fiber without a cross-linker. At higher content of genipin (10%), the elongation at break increases by a factor of 2 and the energy at break increases by a factor of 5. The cross-linking also enables the spinning of microfibers from long straight PNFs, which has not been achieved before. These microfibers have higher stiffness and strength but lower ductility and toughness than those made from curved PNFs. The fibers spun from the two classes of nanofibrils show clear morphological differences. The study demonstrates the production of protein-based microfibers with mechanical properties similar to natural protein-based fibers and provides insights about the role of the nanostructure in the assembly process. American Chemical Society 2022-07-29 2022-08-23 /pmc/articles/PMC9413408/ /pubmed/35904348 http://dx.doi.org/10.1021/acsnano.2c03790 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Ye, Xinchen
Capezza, Antonio J.
Davoodi, Saeed
Wei, Xin-Feng
Andersson, Richard L.
Chumakov, Andrei
Roth, Stephan V.
Langton, Maud
Lundell, Fredrik
Hedenqvist, Mikael S.
Lendel, Christofer
Robust Assembly of Cross-Linked Protein Nanofibrils into Hierarchically Structured Microfibers
title Robust Assembly of Cross-Linked Protein Nanofibrils into Hierarchically Structured Microfibers
title_full Robust Assembly of Cross-Linked Protein Nanofibrils into Hierarchically Structured Microfibers
title_fullStr Robust Assembly of Cross-Linked Protein Nanofibrils into Hierarchically Structured Microfibers
title_full_unstemmed Robust Assembly of Cross-Linked Protein Nanofibrils into Hierarchically Structured Microfibers
title_short Robust Assembly of Cross-Linked Protein Nanofibrils into Hierarchically Structured Microfibers
title_sort robust assembly of cross-linked protein nanofibrils into hierarchically structured microfibers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9413408/
https://www.ncbi.nlm.nih.gov/pubmed/35904348
http://dx.doi.org/10.1021/acsnano.2c03790
work_keys_str_mv AT yexinchen robustassemblyofcrosslinkedproteinnanofibrilsintohierarchicallystructuredmicrofibers
AT capezzaantonioj robustassemblyofcrosslinkedproteinnanofibrilsintohierarchicallystructuredmicrofibers
AT davoodisaeed robustassemblyofcrosslinkedproteinnanofibrilsintohierarchicallystructuredmicrofibers
AT weixinfeng robustassemblyofcrosslinkedproteinnanofibrilsintohierarchicallystructuredmicrofibers
AT anderssonrichardl robustassemblyofcrosslinkedproteinnanofibrilsintohierarchicallystructuredmicrofibers
AT chumakovandrei robustassemblyofcrosslinkedproteinnanofibrilsintohierarchicallystructuredmicrofibers
AT rothstephanv robustassemblyofcrosslinkedproteinnanofibrilsintohierarchicallystructuredmicrofibers
AT langtonmaud robustassemblyofcrosslinkedproteinnanofibrilsintohierarchicallystructuredmicrofibers
AT lundellfredrik robustassemblyofcrosslinkedproteinnanofibrilsintohierarchicallystructuredmicrofibers
AT hedenqvistmikaels robustassemblyofcrosslinkedproteinnanofibrilsintohierarchicallystructuredmicrofibers
AT lendelchristofer robustassemblyofcrosslinkedproteinnanofibrilsintohierarchicallystructuredmicrofibers