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Versatile and Low-Cost Fabrication of Modular Lock-and-Key Microfluidics for Integrated Connector Mixer Using a Stereolithography 3D Printing

We present a low-cost and simple method to fabricate a novel lock-and-key mixer microfluidics using an economic stereolithography (SLA) three-dimensional (3D) printer, which costs less than USD 400 for the investment. The proposed study is promising for a high throughput fabrication module, typicall...

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Autores principales: Anshori, Isa, Lukito, Vincent, Adhawiyah, Rafita, Putri, Delpita, Harimurti, Suksmandhira, Rajab, Tati Latifah Erawati, Pradana, Arfat, Akbar, Mohammad, Syamsunarno, Mas Rizky Anggun Adipurna, Handayani, Murni, Purwidyantri, Agnes, Prabowo, Briliant Adhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9413493/
https://www.ncbi.nlm.nih.gov/pubmed/36014119
http://dx.doi.org/10.3390/mi13081197
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author Anshori, Isa
Lukito, Vincent
Adhawiyah, Rafita
Putri, Delpita
Harimurti, Suksmandhira
Rajab, Tati Latifah Erawati
Pradana, Arfat
Akbar, Mohammad
Syamsunarno, Mas Rizky Anggun Adipurna
Handayani, Murni
Purwidyantri, Agnes
Prabowo, Briliant Adhi
author_facet Anshori, Isa
Lukito, Vincent
Adhawiyah, Rafita
Putri, Delpita
Harimurti, Suksmandhira
Rajab, Tati Latifah Erawati
Pradana, Arfat
Akbar, Mohammad
Syamsunarno, Mas Rizky Anggun Adipurna
Handayani, Murni
Purwidyantri, Agnes
Prabowo, Briliant Adhi
author_sort Anshori, Isa
collection PubMed
description We present a low-cost and simple method to fabricate a novel lock-and-key mixer microfluidics using an economic stereolithography (SLA) three-dimensional (3D) printer, which costs less than USD 400 for the investment. The proposed study is promising for a high throughput fabrication module, typically limited by conventional microfluidics fabrications, such as photolithography and polymer-casting methods. We demonstrate the novel modular lock-and-key mixer for the connector and its chamber modules with optimized parameters, such as exposure condition and printing orientation. In addition, the optimization of post-processing was performed to investigate the reliability of the fabricated hollow structures, which are fundamental to creating a fluidic channel or chamber. We found out that by using an inexpensive 3D printer, the fabricated resolution can be pushed down to 850 µm and 550 µm size for squared- and circled-shapes, respectively, by the gradual hollow structure, applying vertical printing orientation. These strategies opened up the possibility of developing straightforward microfluidics platforms that could replace conventional microfluidics mold fabrication methods, such as photolithography and milling, which are costly and time consuming. Considerably cheap commercial resin and its tiny volume employed for a single printing procedure significantly cut down the estimated fabrication cost to less than 50 cents USD/module. The simulation study unravels the prominent properties of the fabricated devices for biological fluid mixers, such as PBS, urine and plasma blood. This study is eminently prospective toward microfluidics application in clinical biosensing, where disposable, low-cost, high-throughput, and reproducible chips are highly required.
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spelling pubmed-94134932022-08-27 Versatile and Low-Cost Fabrication of Modular Lock-and-Key Microfluidics for Integrated Connector Mixer Using a Stereolithography 3D Printing Anshori, Isa Lukito, Vincent Adhawiyah, Rafita Putri, Delpita Harimurti, Suksmandhira Rajab, Tati Latifah Erawati Pradana, Arfat Akbar, Mohammad Syamsunarno, Mas Rizky Anggun Adipurna Handayani, Murni Purwidyantri, Agnes Prabowo, Briliant Adhi Micromachines (Basel) Article We present a low-cost and simple method to fabricate a novel lock-and-key mixer microfluidics using an economic stereolithography (SLA) three-dimensional (3D) printer, which costs less than USD 400 for the investment. The proposed study is promising for a high throughput fabrication module, typically limited by conventional microfluidics fabrications, such as photolithography and polymer-casting methods. We demonstrate the novel modular lock-and-key mixer for the connector and its chamber modules with optimized parameters, such as exposure condition and printing orientation. In addition, the optimization of post-processing was performed to investigate the reliability of the fabricated hollow structures, which are fundamental to creating a fluidic channel or chamber. We found out that by using an inexpensive 3D printer, the fabricated resolution can be pushed down to 850 µm and 550 µm size for squared- and circled-shapes, respectively, by the gradual hollow structure, applying vertical printing orientation. These strategies opened up the possibility of developing straightforward microfluidics platforms that could replace conventional microfluidics mold fabrication methods, such as photolithography and milling, which are costly and time consuming. Considerably cheap commercial resin and its tiny volume employed for a single printing procedure significantly cut down the estimated fabrication cost to less than 50 cents USD/module. The simulation study unravels the prominent properties of the fabricated devices for biological fluid mixers, such as PBS, urine and plasma blood. This study is eminently prospective toward microfluidics application in clinical biosensing, where disposable, low-cost, high-throughput, and reproducible chips are highly required. MDPI 2022-07-28 /pmc/articles/PMC9413493/ /pubmed/36014119 http://dx.doi.org/10.3390/mi13081197 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Anshori, Isa
Lukito, Vincent
Adhawiyah, Rafita
Putri, Delpita
Harimurti, Suksmandhira
Rajab, Tati Latifah Erawati
Pradana, Arfat
Akbar, Mohammad
Syamsunarno, Mas Rizky Anggun Adipurna
Handayani, Murni
Purwidyantri, Agnes
Prabowo, Briliant Adhi
Versatile and Low-Cost Fabrication of Modular Lock-and-Key Microfluidics for Integrated Connector Mixer Using a Stereolithography 3D Printing
title Versatile and Low-Cost Fabrication of Modular Lock-and-Key Microfluidics for Integrated Connector Mixer Using a Stereolithography 3D Printing
title_full Versatile and Low-Cost Fabrication of Modular Lock-and-Key Microfluidics for Integrated Connector Mixer Using a Stereolithography 3D Printing
title_fullStr Versatile and Low-Cost Fabrication of Modular Lock-and-Key Microfluidics for Integrated Connector Mixer Using a Stereolithography 3D Printing
title_full_unstemmed Versatile and Low-Cost Fabrication of Modular Lock-and-Key Microfluidics for Integrated Connector Mixer Using a Stereolithography 3D Printing
title_short Versatile and Low-Cost Fabrication of Modular Lock-and-Key Microfluidics for Integrated Connector Mixer Using a Stereolithography 3D Printing
title_sort versatile and low-cost fabrication of modular lock-and-key microfluidics for integrated connector mixer using a stereolithography 3d printing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9413493/
https://www.ncbi.nlm.nih.gov/pubmed/36014119
http://dx.doi.org/10.3390/mi13081197
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