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Candidacidal and Antibiofilm Activity of PS1-3 Peptide against Drug-Resistant Candida albicans on Contact Lenses

The recent emergence of antibiotic-resistant fungi has accelerated research on novel antifungal agents. In particular, Candida albicans infections are related to biofilm formation on medical devices, such as catheters, stents, and contact lenses, resulting in high morbidity and mortality. In this st...

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Autores principales: Lee, Jong-Kook, Park, Soyoung, Kim, Young-Min, Guk, Taeuk, Lee, Min-Young, Park, Seong-Cheol, Lee, Jung Ro, Jang, Mi-Kyeong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9413542/
https://www.ncbi.nlm.nih.gov/pubmed/36015228
http://dx.doi.org/10.3390/pharmaceutics14081602
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author Lee, Jong-Kook
Park, Soyoung
Kim, Young-Min
Guk, Taeuk
Lee, Min-Young
Park, Seong-Cheol
Lee, Jung Ro
Jang, Mi-Kyeong
author_facet Lee, Jong-Kook
Park, Soyoung
Kim, Young-Min
Guk, Taeuk
Lee, Min-Young
Park, Seong-Cheol
Lee, Jung Ro
Jang, Mi-Kyeong
author_sort Lee, Jong-Kook
collection PubMed
description The recent emergence of antibiotic-resistant fungi has accelerated research on novel antifungal agents. In particular, Candida albicans infections are related to biofilm formation on medical devices, such as catheters, stents, and contact lenses, resulting in high morbidity and mortality. In this study, we aimed to elucidate the antifungal and antibiofilm effects of a peptide against drug-resistant C. albicans. α-Helical peptides in which the sequence of KWYK was repeated twice and four times, designated peptide series 1 (PS1)-1 and PS1-3, respectively, were generated, and the candidacidal activities of PS1-1, PS1-3, and fluconazole against drug-resistant C. albicans cells were assessed. The PS1-3 peptide showed higher killing activity than PS1-1 or fluconazole and acted via a membranolytic mechanism. In addition, the PS1-3 peptide exhibited more potent activity than PS1-1 and fluconazole in terms of fungal biofilm inhibition and reduction at the minimum fungicidal concentration on the contact lens surface. Overall, these findings established PS1-3 as a potential candidacidal agent for applications on contact lenses.
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spelling pubmed-94135422022-08-27 Candidacidal and Antibiofilm Activity of PS1-3 Peptide against Drug-Resistant Candida albicans on Contact Lenses Lee, Jong-Kook Park, Soyoung Kim, Young-Min Guk, Taeuk Lee, Min-Young Park, Seong-Cheol Lee, Jung Ro Jang, Mi-Kyeong Pharmaceutics Article The recent emergence of antibiotic-resistant fungi has accelerated research on novel antifungal agents. In particular, Candida albicans infections are related to biofilm formation on medical devices, such as catheters, stents, and contact lenses, resulting in high morbidity and mortality. In this study, we aimed to elucidate the antifungal and antibiofilm effects of a peptide against drug-resistant C. albicans. α-Helical peptides in which the sequence of KWYK was repeated twice and four times, designated peptide series 1 (PS1)-1 and PS1-3, respectively, were generated, and the candidacidal activities of PS1-1, PS1-3, and fluconazole against drug-resistant C. albicans cells were assessed. The PS1-3 peptide showed higher killing activity than PS1-1 or fluconazole and acted via a membranolytic mechanism. In addition, the PS1-3 peptide exhibited more potent activity than PS1-1 and fluconazole in terms of fungal biofilm inhibition and reduction at the minimum fungicidal concentration on the contact lens surface. Overall, these findings established PS1-3 as a potential candidacidal agent for applications on contact lenses. MDPI 2022-07-31 /pmc/articles/PMC9413542/ /pubmed/36015228 http://dx.doi.org/10.3390/pharmaceutics14081602 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Jong-Kook
Park, Soyoung
Kim, Young-Min
Guk, Taeuk
Lee, Min-Young
Park, Seong-Cheol
Lee, Jung Ro
Jang, Mi-Kyeong
Candidacidal and Antibiofilm Activity of PS1-3 Peptide against Drug-Resistant Candida albicans on Contact Lenses
title Candidacidal and Antibiofilm Activity of PS1-3 Peptide against Drug-Resistant Candida albicans on Contact Lenses
title_full Candidacidal and Antibiofilm Activity of PS1-3 Peptide against Drug-Resistant Candida albicans on Contact Lenses
title_fullStr Candidacidal and Antibiofilm Activity of PS1-3 Peptide against Drug-Resistant Candida albicans on Contact Lenses
title_full_unstemmed Candidacidal and Antibiofilm Activity of PS1-3 Peptide against Drug-Resistant Candida albicans on Contact Lenses
title_short Candidacidal and Antibiofilm Activity of PS1-3 Peptide against Drug-Resistant Candida albicans on Contact Lenses
title_sort candidacidal and antibiofilm activity of ps1-3 peptide against drug-resistant candida albicans on contact lenses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9413542/
https://www.ncbi.nlm.nih.gov/pubmed/36015228
http://dx.doi.org/10.3390/pharmaceutics14081602
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