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Candidacidal and Antibiofilm Activity of PS1-3 Peptide against Drug-Resistant Candida albicans on Contact Lenses
The recent emergence of antibiotic-resistant fungi has accelerated research on novel antifungal agents. In particular, Candida albicans infections are related to biofilm formation on medical devices, such as catheters, stents, and contact lenses, resulting in high morbidity and mortality. In this st...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9413542/ https://www.ncbi.nlm.nih.gov/pubmed/36015228 http://dx.doi.org/10.3390/pharmaceutics14081602 |
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author | Lee, Jong-Kook Park, Soyoung Kim, Young-Min Guk, Taeuk Lee, Min-Young Park, Seong-Cheol Lee, Jung Ro Jang, Mi-Kyeong |
author_facet | Lee, Jong-Kook Park, Soyoung Kim, Young-Min Guk, Taeuk Lee, Min-Young Park, Seong-Cheol Lee, Jung Ro Jang, Mi-Kyeong |
author_sort | Lee, Jong-Kook |
collection | PubMed |
description | The recent emergence of antibiotic-resistant fungi has accelerated research on novel antifungal agents. In particular, Candida albicans infections are related to biofilm formation on medical devices, such as catheters, stents, and contact lenses, resulting in high morbidity and mortality. In this study, we aimed to elucidate the antifungal and antibiofilm effects of a peptide against drug-resistant C. albicans. α-Helical peptides in which the sequence of KWYK was repeated twice and four times, designated peptide series 1 (PS1)-1 and PS1-3, respectively, were generated, and the candidacidal activities of PS1-1, PS1-3, and fluconazole against drug-resistant C. albicans cells were assessed. The PS1-3 peptide showed higher killing activity than PS1-1 or fluconazole and acted via a membranolytic mechanism. In addition, the PS1-3 peptide exhibited more potent activity than PS1-1 and fluconazole in terms of fungal biofilm inhibition and reduction at the minimum fungicidal concentration on the contact lens surface. Overall, these findings established PS1-3 as a potential candidacidal agent for applications on contact lenses. |
format | Online Article Text |
id | pubmed-9413542 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94135422022-08-27 Candidacidal and Antibiofilm Activity of PS1-3 Peptide against Drug-Resistant Candida albicans on Contact Lenses Lee, Jong-Kook Park, Soyoung Kim, Young-Min Guk, Taeuk Lee, Min-Young Park, Seong-Cheol Lee, Jung Ro Jang, Mi-Kyeong Pharmaceutics Article The recent emergence of antibiotic-resistant fungi has accelerated research on novel antifungal agents. In particular, Candida albicans infections are related to biofilm formation on medical devices, such as catheters, stents, and contact lenses, resulting in high morbidity and mortality. In this study, we aimed to elucidate the antifungal and antibiofilm effects of a peptide against drug-resistant C. albicans. α-Helical peptides in which the sequence of KWYK was repeated twice and four times, designated peptide series 1 (PS1)-1 and PS1-3, respectively, were generated, and the candidacidal activities of PS1-1, PS1-3, and fluconazole against drug-resistant C. albicans cells were assessed. The PS1-3 peptide showed higher killing activity than PS1-1 or fluconazole and acted via a membranolytic mechanism. In addition, the PS1-3 peptide exhibited more potent activity than PS1-1 and fluconazole in terms of fungal biofilm inhibition and reduction at the minimum fungicidal concentration on the contact lens surface. Overall, these findings established PS1-3 as a potential candidacidal agent for applications on contact lenses. MDPI 2022-07-31 /pmc/articles/PMC9413542/ /pubmed/36015228 http://dx.doi.org/10.3390/pharmaceutics14081602 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lee, Jong-Kook Park, Soyoung Kim, Young-Min Guk, Taeuk Lee, Min-Young Park, Seong-Cheol Lee, Jung Ro Jang, Mi-Kyeong Candidacidal and Antibiofilm Activity of PS1-3 Peptide against Drug-Resistant Candida albicans on Contact Lenses |
title | Candidacidal and Antibiofilm Activity of PS1-3 Peptide against Drug-Resistant Candida albicans on Contact Lenses |
title_full | Candidacidal and Antibiofilm Activity of PS1-3 Peptide against Drug-Resistant Candida albicans on Contact Lenses |
title_fullStr | Candidacidal and Antibiofilm Activity of PS1-3 Peptide against Drug-Resistant Candida albicans on Contact Lenses |
title_full_unstemmed | Candidacidal and Antibiofilm Activity of PS1-3 Peptide against Drug-Resistant Candida albicans on Contact Lenses |
title_short | Candidacidal and Antibiofilm Activity of PS1-3 Peptide against Drug-Resistant Candida albicans on Contact Lenses |
title_sort | candidacidal and antibiofilm activity of ps1-3 peptide against drug-resistant candida albicans on contact lenses |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9413542/ https://www.ncbi.nlm.nih.gov/pubmed/36015228 http://dx.doi.org/10.3390/pharmaceutics14081602 |
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