Cargando…

Designing a Novel Peptide-Based Multi-Epitope Vaccine to Evoke a Robust Immune Response against Pathogenic Multidrug-Resistant Providencia heimbachae

Providencia heimbachae, a Gram -ve, rod-shaped, and opportunistic bacteria isolated from the urine, feces, and skin of humans engage in a wide range of infectious diseases such as urinary tract infection (UTI), gastroenteritis, and bacteremia. This bacterium belongs to the Enterobacteriaceae family...

Descripción completa

Detalles Bibliográficos
Autores principales: Naveed, Muhammad, Sheraz, Mohsin, Amin, Aatif, Waseem, Muhammad, Aziz, Tariq, Khan, Ayaz Ali, Ghani, Mustajab, Shahzad, Muhammad, Alruways, Mashael W., Dablool, Anas S., Elazzazy, Ahmed M., Almalki, Abdulraheem Ali, Alamri, Abdulhakeem S., Alhomrani, Majid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9413917/
https://www.ncbi.nlm.nih.gov/pubmed/36016188
http://dx.doi.org/10.3390/vaccines10081300
_version_ 1784775868101427200
author Naveed, Muhammad
Sheraz, Mohsin
Amin, Aatif
Waseem, Muhammad
Aziz, Tariq
Khan, Ayaz Ali
Ghani, Mustajab
Shahzad, Muhammad
Alruways, Mashael W.
Dablool, Anas S.
Elazzazy, Ahmed M.
Almalki, Abdulraheem Ali
Alamri, Abdulhakeem S.
Alhomrani, Majid
author_facet Naveed, Muhammad
Sheraz, Mohsin
Amin, Aatif
Waseem, Muhammad
Aziz, Tariq
Khan, Ayaz Ali
Ghani, Mustajab
Shahzad, Muhammad
Alruways, Mashael W.
Dablool, Anas S.
Elazzazy, Ahmed M.
Almalki, Abdulraheem Ali
Alamri, Abdulhakeem S.
Alhomrani, Majid
author_sort Naveed, Muhammad
collection PubMed
description Providencia heimbachae, a Gram -ve, rod-shaped, and opportunistic bacteria isolated from the urine, feces, and skin of humans engage in a wide range of infectious diseases such as urinary tract infection (UTI), gastroenteritis, and bacteremia. This bacterium belongs to the Enterobacteriaceae family and can resist antibiotics known as multidrug-resistant (MDR), and as such can be life-threatening to humans. After retrieving the whole proteomic sequence of P. heimbachae ATCC 35613, a total of 6 non-homologous and pathogenic proteins were separated. These shortlisted proteins were further analyzed for epitope prediction and found to be highly non-toxic, non-allergenic, and antigenic. From these sequences, T-cell and B-cell (major histocompatibility complex class 1 and 2) epitopes were extracted that provided vaccine constructs, which were then analyzed for population coverage to find its reliability worldwide. The population coverage for MHC-1 and MHC-2 was 98.29% and 81.81%, respectively. Structural prediction was confirmed by validation through physiochemical molecular and immunological characteristics to design a stable and effective vaccine that could give positive results when injected into the body of the organism. Due to this approach, computational vaccines could be an effective alternative against pathogenic microbe since they cover a large population with positive results. In the end, the given findings may help the experimental vaccinologists to develop a very potent and effective peptide-based vaccine.
format Online
Article
Text
id pubmed-9413917
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-94139172022-08-27 Designing a Novel Peptide-Based Multi-Epitope Vaccine to Evoke a Robust Immune Response against Pathogenic Multidrug-Resistant Providencia heimbachae Naveed, Muhammad Sheraz, Mohsin Amin, Aatif Waseem, Muhammad Aziz, Tariq Khan, Ayaz Ali Ghani, Mustajab Shahzad, Muhammad Alruways, Mashael W. Dablool, Anas S. Elazzazy, Ahmed M. Almalki, Abdulraheem Ali Alamri, Abdulhakeem S. Alhomrani, Majid Vaccines (Basel) Article Providencia heimbachae, a Gram -ve, rod-shaped, and opportunistic bacteria isolated from the urine, feces, and skin of humans engage in a wide range of infectious diseases such as urinary tract infection (UTI), gastroenteritis, and bacteremia. This bacterium belongs to the Enterobacteriaceae family and can resist antibiotics known as multidrug-resistant (MDR), and as such can be life-threatening to humans. After retrieving the whole proteomic sequence of P. heimbachae ATCC 35613, a total of 6 non-homologous and pathogenic proteins were separated. These shortlisted proteins were further analyzed for epitope prediction and found to be highly non-toxic, non-allergenic, and antigenic. From these sequences, T-cell and B-cell (major histocompatibility complex class 1 and 2) epitopes were extracted that provided vaccine constructs, which were then analyzed for population coverage to find its reliability worldwide. The population coverage for MHC-1 and MHC-2 was 98.29% and 81.81%, respectively. Structural prediction was confirmed by validation through physiochemical molecular and immunological characteristics to design a stable and effective vaccine that could give positive results when injected into the body of the organism. Due to this approach, computational vaccines could be an effective alternative against pathogenic microbe since they cover a large population with positive results. In the end, the given findings may help the experimental vaccinologists to develop a very potent and effective peptide-based vaccine. MDPI 2022-08-11 /pmc/articles/PMC9413917/ /pubmed/36016188 http://dx.doi.org/10.3390/vaccines10081300 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Naveed, Muhammad
Sheraz, Mohsin
Amin, Aatif
Waseem, Muhammad
Aziz, Tariq
Khan, Ayaz Ali
Ghani, Mustajab
Shahzad, Muhammad
Alruways, Mashael W.
Dablool, Anas S.
Elazzazy, Ahmed M.
Almalki, Abdulraheem Ali
Alamri, Abdulhakeem S.
Alhomrani, Majid
Designing a Novel Peptide-Based Multi-Epitope Vaccine to Evoke a Robust Immune Response against Pathogenic Multidrug-Resistant Providencia heimbachae
title Designing a Novel Peptide-Based Multi-Epitope Vaccine to Evoke a Robust Immune Response against Pathogenic Multidrug-Resistant Providencia heimbachae
title_full Designing a Novel Peptide-Based Multi-Epitope Vaccine to Evoke a Robust Immune Response against Pathogenic Multidrug-Resistant Providencia heimbachae
title_fullStr Designing a Novel Peptide-Based Multi-Epitope Vaccine to Evoke a Robust Immune Response against Pathogenic Multidrug-Resistant Providencia heimbachae
title_full_unstemmed Designing a Novel Peptide-Based Multi-Epitope Vaccine to Evoke a Robust Immune Response against Pathogenic Multidrug-Resistant Providencia heimbachae
title_short Designing a Novel Peptide-Based Multi-Epitope Vaccine to Evoke a Robust Immune Response against Pathogenic Multidrug-Resistant Providencia heimbachae
title_sort designing a novel peptide-based multi-epitope vaccine to evoke a robust immune response against pathogenic multidrug-resistant providencia heimbachae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9413917/
https://www.ncbi.nlm.nih.gov/pubmed/36016188
http://dx.doi.org/10.3390/vaccines10081300
work_keys_str_mv AT naveedmuhammad designinganovelpeptidebasedmultiepitopevaccinetoevokearobustimmuneresponseagainstpathogenicmultidrugresistantprovidenciaheimbachae
AT sherazmohsin designinganovelpeptidebasedmultiepitopevaccinetoevokearobustimmuneresponseagainstpathogenicmultidrugresistantprovidenciaheimbachae
AT aminaatif designinganovelpeptidebasedmultiepitopevaccinetoevokearobustimmuneresponseagainstpathogenicmultidrugresistantprovidenciaheimbachae
AT waseemmuhammad designinganovelpeptidebasedmultiepitopevaccinetoevokearobustimmuneresponseagainstpathogenicmultidrugresistantprovidenciaheimbachae
AT aziztariq designinganovelpeptidebasedmultiepitopevaccinetoevokearobustimmuneresponseagainstpathogenicmultidrugresistantprovidenciaheimbachae
AT khanayazali designinganovelpeptidebasedmultiepitopevaccinetoevokearobustimmuneresponseagainstpathogenicmultidrugresistantprovidenciaheimbachae
AT ghanimustajab designinganovelpeptidebasedmultiepitopevaccinetoevokearobustimmuneresponseagainstpathogenicmultidrugresistantprovidenciaheimbachae
AT shahzadmuhammad designinganovelpeptidebasedmultiepitopevaccinetoevokearobustimmuneresponseagainstpathogenicmultidrugresistantprovidenciaheimbachae
AT alruwaysmashaelw designinganovelpeptidebasedmultiepitopevaccinetoevokearobustimmuneresponseagainstpathogenicmultidrugresistantprovidenciaheimbachae
AT dabloolanass designinganovelpeptidebasedmultiepitopevaccinetoevokearobustimmuneresponseagainstpathogenicmultidrugresistantprovidenciaheimbachae
AT elazzazyahmedm designinganovelpeptidebasedmultiepitopevaccinetoevokearobustimmuneresponseagainstpathogenicmultidrugresistantprovidenciaheimbachae
AT almalkiabdulraheemali designinganovelpeptidebasedmultiepitopevaccinetoevokearobustimmuneresponseagainstpathogenicmultidrugresistantprovidenciaheimbachae
AT alamriabdulhakeems designinganovelpeptidebasedmultiepitopevaccinetoevokearobustimmuneresponseagainstpathogenicmultidrugresistantprovidenciaheimbachae
AT alhomranimajid designinganovelpeptidebasedmultiepitopevaccinetoevokearobustimmuneresponseagainstpathogenicmultidrugresistantprovidenciaheimbachae