First Report of ‘Candidatus Mycoplasma haematomacacae’ in Laboratory-Kept Rhesus Monkeys (Macaca mulatta) Maintained in Rio de Janeiro, Brazil

SIMPLE SUMMARY: Health assessment in animals used in research studies are essential, since only animals that present no diseases are considered suitable for these purposes. In laboratory kept animals, a bacterium that infects red blood cells, named hemotropic Mycoplasma (also called hemoplasmas), has been described as problem for research studies. Different hemoplasma species have been detected infecting monkeys from Brazil. However, the occurrence of these bacteria in monkeys maintained in laboratory in Brazil have never been described. Accordingly, this study aimed: (1) to screen laboratory-kept rhesus monkeys for hemoplasmas; (2) to verify if any of the hemoplasma-positive animals demonstrate a decrease in their red blood cells counts; and (3) to investigate the genetic diversity of hemoplasma species in monkeys from Brazil. Five out of eight (62.5%) rhesus monkeys tested positive for hemoplasmas using a technique that detects DNA from these bacteria in monkey’s blood. Further analysis demonstrated that rhesus monkeys were infected by a species named ‘Candidatus Mycoplasma haematomacacae’ that had already been described occurring in monkeys from Japan and USA. Although no decreases on red blood cells count were perceived in rhesus monkeys evaluated herein, future studies are needed to elucidate if ‘Ca. M. haematomacacae’ is a problem for research studies that use rhesus monkeys. ABSTRACT: Health monitoring programs in animals used as experimental models are essential, since only disease-free subjects are considered suitable for research purposes. In laboratory-kept animals, hemoplasmas have been described as an important confounding variable. Different hemoplasma species have been detected infecting non-human primates (NHP) from Brazil. However, the occurrence of hemoplasma species in laboratory-kept NHP in Brazil has not-yet been assessed. Accordingly, this study aimed (i) to screen laboratory-kept rhesus monkeys for hemoplasmas, (ii) to verify if any of the hemoplasma-positive animals demonstrate hematological abnormalities, and (iii) to assess the genotype diversity of hemoplasma species in NHP from Brazil. Five out of eight (62.5%; 95% CI: 3.05–8.63) rhesus monkeys tested positive for hemotropic Mycoplasma spp. by PCR. Sequencing, phylogenetic, distance, and genotype diversity analyses of partial 16S rRNA gene demonstrate that rhesus monkeys were infected by ‘Candidatus Mycoplasma haematomacacae’ (formerly ‘Candidatus Mycoplasma haemomacaque’). Assessments of partial 16S rRNA diversity of hemoplasma species in NHP suggest that at least four genetically diverse groups may occur in Brazil. Although no hematological abnormalities were demonstrated in rhesus monkeys evaluated herein, future studies are needed to elucidate the influence of ‘Ca. M. haematomacacae’ as a confounding variable on research studies.