Cargando…

Association of Indocyanine Green with Chitosan Oleate Coated PLGA Nanoparticles for Photodynamic Therapy

Indocyanine green (ICG) is a safe dye widely used in the biomedical field. Its photodynamic effect (PDT), originating from laser irradiation at 803 nm, opens interesting perspectives in theranostic applications. To overcome its low water stability, ICG can be shielded with nanoparticles (NPs). In th...

Descripción completa

Detalles Bibliográficos
Autores principales: Miele, Dalila, Sorrenti, Milena, Catenacci, Laura, Minzioni, Paolo, Marrubini, Giorgio, Amendola, Valeria, Maestri, Marcello, Giunchedi, Paolo, Bonferoni, Maria Cristina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9414095/
https://www.ncbi.nlm.nih.gov/pubmed/36015366
http://dx.doi.org/10.3390/pharmaceutics14081740
_version_ 1784775910779518976
author Miele, Dalila
Sorrenti, Milena
Catenacci, Laura
Minzioni, Paolo
Marrubini, Giorgio
Amendola, Valeria
Maestri, Marcello
Giunchedi, Paolo
Bonferoni, Maria Cristina
author_facet Miele, Dalila
Sorrenti, Milena
Catenacci, Laura
Minzioni, Paolo
Marrubini, Giorgio
Amendola, Valeria
Maestri, Marcello
Giunchedi, Paolo
Bonferoni, Maria Cristina
author_sort Miele, Dalila
collection PubMed
description Indocyanine green (ICG) is a safe dye widely used in the biomedical field. Its photodynamic effect (PDT), originating from laser irradiation at 803 nm, opens interesting perspectives in theranostic applications. To overcome its low water stability, ICG can be shielded with nanoparticles (NPs). In this work, previously developed NPs based on poly lactic-co-glycolic acid (PLGA) coated with chitosan oleate (CS-OA) and loaded with resveratrol as a hydrophobic model drug have been proposed as an ICG carrier. These systems have been selected for their observed immunostimulatory properties. The possible loading of the dye by adsorption onto NP surface by electrostatic interaction was studied here in comparison with the encapsulation into the PLGA core. The ICG-chitosan (CS) interaction has been characterized by spectrophotometry, spectroscopy and in-cell in vitro assays. Fluorescence quenching was observed due to the ionic interaction between ICG and CS and was studied considering the dye:polymer stoichiometry and the effect of the NP dilution in cell culture medium (DMEM). The NP systems have been compared in vitro, assessing their behaviour in Caco-2 cell lines. A reduction in cell viability was observed after irradiation of ICG associated with NPs, evident also for the samples loaded by adsorption. These findings open the opportunity to exploit the association of PDT’s effect on ICG with the properties of CS-OA coated NPs, whose immunostimulatory effect can be associated with PDT mechanism in cancer therapy.
format Online
Article
Text
id pubmed-9414095
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-94140952022-08-27 Association of Indocyanine Green with Chitosan Oleate Coated PLGA Nanoparticles for Photodynamic Therapy Miele, Dalila Sorrenti, Milena Catenacci, Laura Minzioni, Paolo Marrubini, Giorgio Amendola, Valeria Maestri, Marcello Giunchedi, Paolo Bonferoni, Maria Cristina Pharmaceutics Article Indocyanine green (ICG) is a safe dye widely used in the biomedical field. Its photodynamic effect (PDT), originating from laser irradiation at 803 nm, opens interesting perspectives in theranostic applications. To overcome its low water stability, ICG can be shielded with nanoparticles (NPs). In this work, previously developed NPs based on poly lactic-co-glycolic acid (PLGA) coated with chitosan oleate (CS-OA) and loaded with resveratrol as a hydrophobic model drug have been proposed as an ICG carrier. These systems have been selected for their observed immunostimulatory properties. The possible loading of the dye by adsorption onto NP surface by electrostatic interaction was studied here in comparison with the encapsulation into the PLGA core. The ICG-chitosan (CS) interaction has been characterized by spectrophotometry, spectroscopy and in-cell in vitro assays. Fluorescence quenching was observed due to the ionic interaction between ICG and CS and was studied considering the dye:polymer stoichiometry and the effect of the NP dilution in cell culture medium (DMEM). The NP systems have been compared in vitro, assessing their behaviour in Caco-2 cell lines. A reduction in cell viability was observed after irradiation of ICG associated with NPs, evident also for the samples loaded by adsorption. These findings open the opportunity to exploit the association of PDT’s effect on ICG with the properties of CS-OA coated NPs, whose immunostimulatory effect can be associated with PDT mechanism in cancer therapy. MDPI 2022-08-20 /pmc/articles/PMC9414095/ /pubmed/36015366 http://dx.doi.org/10.3390/pharmaceutics14081740 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Miele, Dalila
Sorrenti, Milena
Catenacci, Laura
Minzioni, Paolo
Marrubini, Giorgio
Amendola, Valeria
Maestri, Marcello
Giunchedi, Paolo
Bonferoni, Maria Cristina
Association of Indocyanine Green with Chitosan Oleate Coated PLGA Nanoparticles for Photodynamic Therapy
title Association of Indocyanine Green with Chitosan Oleate Coated PLGA Nanoparticles for Photodynamic Therapy
title_full Association of Indocyanine Green with Chitosan Oleate Coated PLGA Nanoparticles for Photodynamic Therapy
title_fullStr Association of Indocyanine Green with Chitosan Oleate Coated PLGA Nanoparticles for Photodynamic Therapy
title_full_unstemmed Association of Indocyanine Green with Chitosan Oleate Coated PLGA Nanoparticles for Photodynamic Therapy
title_short Association of Indocyanine Green with Chitosan Oleate Coated PLGA Nanoparticles for Photodynamic Therapy
title_sort association of indocyanine green with chitosan oleate coated plga nanoparticles for photodynamic therapy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9414095/
https://www.ncbi.nlm.nih.gov/pubmed/36015366
http://dx.doi.org/10.3390/pharmaceutics14081740
work_keys_str_mv AT mieledalila associationofindocyaninegreenwithchitosanoleatecoatedplgananoparticlesforphotodynamictherapy
AT sorrentimilena associationofindocyaninegreenwithchitosanoleatecoatedplgananoparticlesforphotodynamictherapy
AT catenaccilaura associationofindocyaninegreenwithchitosanoleatecoatedplgananoparticlesforphotodynamictherapy
AT minzionipaolo associationofindocyaninegreenwithchitosanoleatecoatedplgananoparticlesforphotodynamictherapy
AT marrubinigiorgio associationofindocyaninegreenwithchitosanoleatecoatedplgananoparticlesforphotodynamictherapy
AT amendolavaleria associationofindocyaninegreenwithchitosanoleatecoatedplgananoparticlesforphotodynamictherapy
AT maestrimarcello associationofindocyaninegreenwithchitosanoleatecoatedplgananoparticlesforphotodynamictherapy
AT giunchedipaolo associationofindocyaninegreenwithchitosanoleatecoatedplgananoparticlesforphotodynamictherapy
AT bonferonimariacristina associationofindocyaninegreenwithchitosanoleatecoatedplgananoparticlesforphotodynamictherapy