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Molecular Approach for the Laboratory Diagnosis of Periprosthetic Joint Infections
The incidence of total joint arthroplasty is increasing over time since the last decade and expected to be more than 4 million by 2030. As a consequence, the detection of infections associated with surgical interventions is increasing and prosthetic joint infections are representing both a clinicall...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9414264/ https://www.ncbi.nlm.nih.gov/pubmed/36013991 http://dx.doi.org/10.3390/microorganisms10081573 |
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author | Gatti, Giulia Taddei, Francesca Brandolini, Martina Mancini, Andrea Denicolò, Agnese Congestrì, Francesco Manera, Martina Arfilli, Valentina Battisti, Arianna Zannoli, Silvia Marino, Maria Michela Marzucco, Anna Morotti, Manuela Grumiro, Laura Scalcione, Agata Dirani, Giorgio Cricca, Monica Sambri, Vittorio |
author_facet | Gatti, Giulia Taddei, Francesca Brandolini, Martina Mancini, Andrea Denicolò, Agnese Congestrì, Francesco Manera, Martina Arfilli, Valentina Battisti, Arianna Zannoli, Silvia Marino, Maria Michela Marzucco, Anna Morotti, Manuela Grumiro, Laura Scalcione, Agata Dirani, Giorgio Cricca, Monica Sambri, Vittorio |
author_sort | Gatti, Giulia |
collection | PubMed |
description | The incidence of total joint arthroplasty is increasing over time since the last decade and expected to be more than 4 million by 2030. As a consequence, the detection of infections associated with surgical interventions is increasing and prosthetic joint infections are representing both a clinically and economically challenging problem. Many pathogens, from bacteria to fungi, elicit the immune system response and produce a polymeric matrix, the biofilm, that serves as their protection. In the last years, the implementation of diagnostic methodologies reduced the error rate and the turn-around time: polymerase chain reaction, targeted or broad-spectrum, and next-generation sequencing have been introduced and they represent a robust approach nowadays that frees laboratories from the unique approach based on culture-based techniques. |
format | Online Article Text |
id | pubmed-9414264 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94142642022-08-27 Molecular Approach for the Laboratory Diagnosis of Periprosthetic Joint Infections Gatti, Giulia Taddei, Francesca Brandolini, Martina Mancini, Andrea Denicolò, Agnese Congestrì, Francesco Manera, Martina Arfilli, Valentina Battisti, Arianna Zannoli, Silvia Marino, Maria Michela Marzucco, Anna Morotti, Manuela Grumiro, Laura Scalcione, Agata Dirani, Giorgio Cricca, Monica Sambri, Vittorio Microorganisms Review The incidence of total joint arthroplasty is increasing over time since the last decade and expected to be more than 4 million by 2030. As a consequence, the detection of infections associated with surgical interventions is increasing and prosthetic joint infections are representing both a clinically and economically challenging problem. Many pathogens, from bacteria to fungi, elicit the immune system response and produce a polymeric matrix, the biofilm, that serves as their protection. In the last years, the implementation of diagnostic methodologies reduced the error rate and the turn-around time: polymerase chain reaction, targeted or broad-spectrum, and next-generation sequencing have been introduced and they represent a robust approach nowadays that frees laboratories from the unique approach based on culture-based techniques. MDPI 2022-08-05 /pmc/articles/PMC9414264/ /pubmed/36013991 http://dx.doi.org/10.3390/microorganisms10081573 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Gatti, Giulia Taddei, Francesca Brandolini, Martina Mancini, Andrea Denicolò, Agnese Congestrì, Francesco Manera, Martina Arfilli, Valentina Battisti, Arianna Zannoli, Silvia Marino, Maria Michela Marzucco, Anna Morotti, Manuela Grumiro, Laura Scalcione, Agata Dirani, Giorgio Cricca, Monica Sambri, Vittorio Molecular Approach for the Laboratory Diagnosis of Periprosthetic Joint Infections |
title | Molecular Approach for the Laboratory Diagnosis of Periprosthetic Joint Infections |
title_full | Molecular Approach for the Laboratory Diagnosis of Periprosthetic Joint Infections |
title_fullStr | Molecular Approach for the Laboratory Diagnosis of Periprosthetic Joint Infections |
title_full_unstemmed | Molecular Approach for the Laboratory Diagnosis of Periprosthetic Joint Infections |
title_short | Molecular Approach for the Laboratory Diagnosis of Periprosthetic Joint Infections |
title_sort | molecular approach for the laboratory diagnosis of periprosthetic joint infections |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9414264/ https://www.ncbi.nlm.nih.gov/pubmed/36013991 http://dx.doi.org/10.3390/microorganisms10081573 |
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