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Molecular Approach for the Laboratory Diagnosis of Periprosthetic Joint Infections

The incidence of total joint arthroplasty is increasing over time since the last decade and expected to be more than 4 million by 2030. As a consequence, the detection of infections associated with surgical interventions is increasing and prosthetic joint infections are representing both a clinicall...

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Autores principales: Gatti, Giulia, Taddei, Francesca, Brandolini, Martina, Mancini, Andrea, Denicolò, Agnese, Congestrì, Francesco, Manera, Martina, Arfilli, Valentina, Battisti, Arianna, Zannoli, Silvia, Marino, Maria Michela, Marzucco, Anna, Morotti, Manuela, Grumiro, Laura, Scalcione, Agata, Dirani, Giorgio, Cricca, Monica, Sambri, Vittorio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9414264/
https://www.ncbi.nlm.nih.gov/pubmed/36013991
http://dx.doi.org/10.3390/microorganisms10081573
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author Gatti, Giulia
Taddei, Francesca
Brandolini, Martina
Mancini, Andrea
Denicolò, Agnese
Congestrì, Francesco
Manera, Martina
Arfilli, Valentina
Battisti, Arianna
Zannoli, Silvia
Marino, Maria Michela
Marzucco, Anna
Morotti, Manuela
Grumiro, Laura
Scalcione, Agata
Dirani, Giorgio
Cricca, Monica
Sambri, Vittorio
author_facet Gatti, Giulia
Taddei, Francesca
Brandolini, Martina
Mancini, Andrea
Denicolò, Agnese
Congestrì, Francesco
Manera, Martina
Arfilli, Valentina
Battisti, Arianna
Zannoli, Silvia
Marino, Maria Michela
Marzucco, Anna
Morotti, Manuela
Grumiro, Laura
Scalcione, Agata
Dirani, Giorgio
Cricca, Monica
Sambri, Vittorio
author_sort Gatti, Giulia
collection PubMed
description The incidence of total joint arthroplasty is increasing over time since the last decade and expected to be more than 4 million by 2030. As a consequence, the detection of infections associated with surgical interventions is increasing and prosthetic joint infections are representing both a clinically and economically challenging problem. Many pathogens, from bacteria to fungi, elicit the immune system response and produce a polymeric matrix, the biofilm, that serves as their protection. In the last years, the implementation of diagnostic methodologies reduced the error rate and the turn-around time: polymerase chain reaction, targeted or broad-spectrum, and next-generation sequencing have been introduced and they represent a robust approach nowadays that frees laboratories from the unique approach based on culture-based techniques.
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spelling pubmed-94142642022-08-27 Molecular Approach for the Laboratory Diagnosis of Periprosthetic Joint Infections Gatti, Giulia Taddei, Francesca Brandolini, Martina Mancini, Andrea Denicolò, Agnese Congestrì, Francesco Manera, Martina Arfilli, Valentina Battisti, Arianna Zannoli, Silvia Marino, Maria Michela Marzucco, Anna Morotti, Manuela Grumiro, Laura Scalcione, Agata Dirani, Giorgio Cricca, Monica Sambri, Vittorio Microorganisms Review The incidence of total joint arthroplasty is increasing over time since the last decade and expected to be more than 4 million by 2030. As a consequence, the detection of infections associated with surgical interventions is increasing and prosthetic joint infections are representing both a clinically and economically challenging problem. Many pathogens, from bacteria to fungi, elicit the immune system response and produce a polymeric matrix, the biofilm, that serves as their protection. In the last years, the implementation of diagnostic methodologies reduced the error rate and the turn-around time: polymerase chain reaction, targeted or broad-spectrum, and next-generation sequencing have been introduced and they represent a robust approach nowadays that frees laboratories from the unique approach based on culture-based techniques. MDPI 2022-08-05 /pmc/articles/PMC9414264/ /pubmed/36013991 http://dx.doi.org/10.3390/microorganisms10081573 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Gatti, Giulia
Taddei, Francesca
Brandolini, Martina
Mancini, Andrea
Denicolò, Agnese
Congestrì, Francesco
Manera, Martina
Arfilli, Valentina
Battisti, Arianna
Zannoli, Silvia
Marino, Maria Michela
Marzucco, Anna
Morotti, Manuela
Grumiro, Laura
Scalcione, Agata
Dirani, Giorgio
Cricca, Monica
Sambri, Vittorio
Molecular Approach for the Laboratory Diagnosis of Periprosthetic Joint Infections
title Molecular Approach for the Laboratory Diagnosis of Periprosthetic Joint Infections
title_full Molecular Approach for the Laboratory Diagnosis of Periprosthetic Joint Infections
title_fullStr Molecular Approach for the Laboratory Diagnosis of Periprosthetic Joint Infections
title_full_unstemmed Molecular Approach for the Laboratory Diagnosis of Periprosthetic Joint Infections
title_short Molecular Approach for the Laboratory Diagnosis of Periprosthetic Joint Infections
title_sort molecular approach for the laboratory diagnosis of periprosthetic joint infections
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9414264/
https://www.ncbi.nlm.nih.gov/pubmed/36013991
http://dx.doi.org/10.3390/microorganisms10081573
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