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Isolation and Characterization of the Lytic Pseudoxanthomonas kaohsiungensi Phage PW916

The emergence of multidrug-resistant bacterial pathogens poses a serious global health threat. While patient infections by the opportunistic human pathogen Pseudoxanthomonas spp. have been increasingly reported worldwide, no phage associated with this bacterial genus has yet been isolated and report...

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Autores principales: Wen, Chang, Ai, Chaofan, Lu, Shiyun, Yang, Qiue, Liao, Hanpeng, Zhou, Shungui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9414467/
https://www.ncbi.nlm.nih.gov/pubmed/36016331
http://dx.doi.org/10.3390/v14081709
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author Wen, Chang
Ai, Chaofan
Lu, Shiyun
Yang, Qiue
Liao, Hanpeng
Zhou, Shungui
author_facet Wen, Chang
Ai, Chaofan
Lu, Shiyun
Yang, Qiue
Liao, Hanpeng
Zhou, Shungui
author_sort Wen, Chang
collection PubMed
description The emergence of multidrug-resistant bacterial pathogens poses a serious global health threat. While patient infections by the opportunistic human pathogen Pseudoxanthomonas spp. have been increasingly reported worldwide, no phage associated with this bacterial genus has yet been isolated and reported. In this study, we isolated and characterized the novel phage PW916 to subsequently be used to lyse the multidrug-resistant Pseudoxanthomonas kaohsiungensi which was isolated from soil samples obtained from Chongqing, China. We studied the morphological features, thermal stability, pH stability, optimal multiplicity of infection, and genomic sequence of phage PW916. Transmission electron microscopy revealed the morphology of PW916 and indicated it to belong to the Siphoviridae family, with the morphological characteristics of a rounded head and a long noncontractile tail. The optimal multiplicity of infection of PW916 was 0.1. Moreover, PW916 was found to be stable under a wide range of temperatures (4–60 °C), pH (4–11) as well as treatment with 1% (v/w) chloroform. The genome of PW916 was determined to be a circular double-stranded structure with a length of 47,760 bp, containing 64 open reading frames that encoded functional and structural proteins, while no antibiotic resistance nor virulence factor genes were detected. The genomic sequencing and phylogenetic tree analysis showed that PW916 was a novel phage belonging to the Siphoviridae family that was closely related to the Stenotrophomonas phage. This is the first study to identify a novel phage infecting the multidrug-resistant P. kaohsiungensi and the findings provide insight into the potential application of PW916 in future phage therapies.
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spelling pubmed-94144672022-08-27 Isolation and Characterization of the Lytic Pseudoxanthomonas kaohsiungensi Phage PW916 Wen, Chang Ai, Chaofan Lu, Shiyun Yang, Qiue Liao, Hanpeng Zhou, Shungui Viruses Article The emergence of multidrug-resistant bacterial pathogens poses a serious global health threat. While patient infections by the opportunistic human pathogen Pseudoxanthomonas spp. have been increasingly reported worldwide, no phage associated with this bacterial genus has yet been isolated and reported. In this study, we isolated and characterized the novel phage PW916 to subsequently be used to lyse the multidrug-resistant Pseudoxanthomonas kaohsiungensi which was isolated from soil samples obtained from Chongqing, China. We studied the morphological features, thermal stability, pH stability, optimal multiplicity of infection, and genomic sequence of phage PW916. Transmission electron microscopy revealed the morphology of PW916 and indicated it to belong to the Siphoviridae family, with the morphological characteristics of a rounded head and a long noncontractile tail. The optimal multiplicity of infection of PW916 was 0.1. Moreover, PW916 was found to be stable under a wide range of temperatures (4–60 °C), pH (4–11) as well as treatment with 1% (v/w) chloroform. The genome of PW916 was determined to be a circular double-stranded structure with a length of 47,760 bp, containing 64 open reading frames that encoded functional and structural proteins, while no antibiotic resistance nor virulence factor genes were detected. The genomic sequencing and phylogenetic tree analysis showed that PW916 was a novel phage belonging to the Siphoviridae family that was closely related to the Stenotrophomonas phage. This is the first study to identify a novel phage infecting the multidrug-resistant P. kaohsiungensi and the findings provide insight into the potential application of PW916 in future phage therapies. MDPI 2022-08-02 /pmc/articles/PMC9414467/ /pubmed/36016331 http://dx.doi.org/10.3390/v14081709 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wen, Chang
Ai, Chaofan
Lu, Shiyun
Yang, Qiue
Liao, Hanpeng
Zhou, Shungui
Isolation and Characterization of the Lytic Pseudoxanthomonas kaohsiungensi Phage PW916
title Isolation and Characterization of the Lytic Pseudoxanthomonas kaohsiungensi Phage PW916
title_full Isolation and Characterization of the Lytic Pseudoxanthomonas kaohsiungensi Phage PW916
title_fullStr Isolation and Characterization of the Lytic Pseudoxanthomonas kaohsiungensi Phage PW916
title_full_unstemmed Isolation and Characterization of the Lytic Pseudoxanthomonas kaohsiungensi Phage PW916
title_short Isolation and Characterization of the Lytic Pseudoxanthomonas kaohsiungensi Phage PW916
title_sort isolation and characterization of the lytic pseudoxanthomonas kaohsiungensi phage pw916
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9414467/
https://www.ncbi.nlm.nih.gov/pubmed/36016331
http://dx.doi.org/10.3390/v14081709
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