Cargando…

Acoustic Manipulation of Intraocular Particles

Various conditions cause dispersions of particulate matter to circulate inside the anterior chamber of a human eye. These dispersed particles might reduce visual acuity or promote elevation of intraocular pressure (IOP), causing secondary complications such as particle related glaucoma, which is a m...

Descripción completa

Detalles Bibliográficos
Autores principales: Leshno, Ari, Kenigsberg, Avraham, Peleg-Levy, Heli, Piperno, Silvia, Skaat, Alon, Shpaisman, Hagay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9414468/
https://www.ncbi.nlm.nih.gov/pubmed/36014284
http://dx.doi.org/10.3390/mi13081362
_version_ 1784775994975977472
author Leshno, Ari
Kenigsberg, Avraham
Peleg-Levy, Heli
Piperno, Silvia
Skaat, Alon
Shpaisman, Hagay
author_facet Leshno, Ari
Kenigsberg, Avraham
Peleg-Levy, Heli
Piperno, Silvia
Skaat, Alon
Shpaisman, Hagay
author_sort Leshno, Ari
collection PubMed
description Various conditions cause dispersions of particulate matter to circulate inside the anterior chamber of a human eye. These dispersed particles might reduce visual acuity or promote elevation of intraocular pressure (IOP), causing secondary complications such as particle related glaucoma, which is a major cause of blindness. Medical and surgical treatment options are available to manage these complications, yet preventive measures are not currently available. Conceptually, manipulating these dispersed particles in a way that reduces their negative impact could prevent these complications. However, as the eye is a closed system, manipulating dispersed particles in it is challenging. Standing acoustic waves have been previously shown to be a versatile tool for manipulation of bioparticles from nano-sized extracellular vesicles up to millimeter-sized organisms. Here we introduce for the first time a novel method utilizing standing acoustic waves to noninvasively manipulate intraocular particles inside the anterior chamber. Using a cylindrical acoustic resonator, we show ex vivo manipulation of pigmentary particles inside porcine eyes. We study the effect of wave intensity over time and rule out temperature changes that could damage tissues. Optical coherence tomography and histologic evaluations show no signs of damage or any other side effect that could be attributed to acoustic manipulation. Finally, we lay out a clear pathway to how this technique can be used as a non-invasive tool for preventing secondary glaucoma. This concept has the potential to control and arrange intraocular particles in specific locations without causing any damage to ocular tissue and allow aqueous humor normal outflow which is crucial for maintaining proper IOP levels.
format Online
Article
Text
id pubmed-9414468
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-94144682022-08-27 Acoustic Manipulation of Intraocular Particles Leshno, Ari Kenigsberg, Avraham Peleg-Levy, Heli Piperno, Silvia Skaat, Alon Shpaisman, Hagay Micromachines (Basel) Article Various conditions cause dispersions of particulate matter to circulate inside the anterior chamber of a human eye. These dispersed particles might reduce visual acuity or promote elevation of intraocular pressure (IOP), causing secondary complications such as particle related glaucoma, which is a major cause of blindness. Medical and surgical treatment options are available to manage these complications, yet preventive measures are not currently available. Conceptually, manipulating these dispersed particles in a way that reduces their negative impact could prevent these complications. However, as the eye is a closed system, manipulating dispersed particles in it is challenging. Standing acoustic waves have been previously shown to be a versatile tool for manipulation of bioparticles from nano-sized extracellular vesicles up to millimeter-sized organisms. Here we introduce for the first time a novel method utilizing standing acoustic waves to noninvasively manipulate intraocular particles inside the anterior chamber. Using a cylindrical acoustic resonator, we show ex vivo manipulation of pigmentary particles inside porcine eyes. We study the effect of wave intensity over time and rule out temperature changes that could damage tissues. Optical coherence tomography and histologic evaluations show no signs of damage or any other side effect that could be attributed to acoustic manipulation. Finally, we lay out a clear pathway to how this technique can be used as a non-invasive tool for preventing secondary glaucoma. This concept has the potential to control and arrange intraocular particles in specific locations without causing any damage to ocular tissue and allow aqueous humor normal outflow which is crucial for maintaining proper IOP levels. MDPI 2022-08-21 /pmc/articles/PMC9414468/ /pubmed/36014284 http://dx.doi.org/10.3390/mi13081362 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Leshno, Ari
Kenigsberg, Avraham
Peleg-Levy, Heli
Piperno, Silvia
Skaat, Alon
Shpaisman, Hagay
Acoustic Manipulation of Intraocular Particles
title Acoustic Manipulation of Intraocular Particles
title_full Acoustic Manipulation of Intraocular Particles
title_fullStr Acoustic Manipulation of Intraocular Particles
title_full_unstemmed Acoustic Manipulation of Intraocular Particles
title_short Acoustic Manipulation of Intraocular Particles
title_sort acoustic manipulation of intraocular particles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9414468/
https://www.ncbi.nlm.nih.gov/pubmed/36014284
http://dx.doi.org/10.3390/mi13081362
work_keys_str_mv AT leshnoari acousticmanipulationofintraocularparticles
AT kenigsbergavraham acousticmanipulationofintraocularparticles
AT peleglevyheli acousticmanipulationofintraocularparticles
AT pipernosilvia acousticmanipulationofintraocularparticles
AT skaatalon acousticmanipulationofintraocularparticles
AT shpaismanhagay acousticmanipulationofintraocularparticles